DOI QR코드

DOI QR Code

The Effect of Quercetin in Corneal Opacity Induced by Mitomycin-C

  • Lee, Yoon Jeong (Department of Optometry & Vision Science, Kyungwoon University, Department of Life Sciences, Yeungnam University)
  • 투고 : 2014.08.19
  • 심사 : 2014.09.24
  • 발행 : 2014.09.30

초록

This study examined the effects of quercetin on corneal opacity caused by corneal edema by suppressing the damage on corneal endothelial cell, which was induced by mitomycin-C (MMC). In the MMC-treated group, the number of keratocytes was noticeably fewer compared to that of other groups. Although this group showed normal amount of fiber in the corneal stroma, the thickness was shown to be very thick and the alignment of the corneal endothelial cells that worked as the barrier against aqueous humor was irregular. According to such results, it was known that corneal opacity induced by MMC is not caused by proliferation of keratocytes, but by corneal edema triggered by the infiltration of aqueous humor. In the MMC+quercetin and quercetin+MMC-treated groups, the number of keratocytes was higher and polymorphonuclear leukocytes infilteration was lower significantly compared to that of the MMC-treated group. Although the amounts of fiber and endothelioid cell arrangement were normal, there was more space observed in the corneal stroma. Nonetheless, these groups showed significantly lower stromal thickness compared to that of the MMC group. In conclusion, quercetin has the effect on the reduction of corneal opacity caused by corneal edema that work MMC-induced damage to the corneal endothelial cells.

키워드

참고문헌

  1. Anton R (1988) Flavonoids and traditional medicine. Prog. Clin. Biol. Res. 280, 423-439.
  2. Brown J P (1980) A review of the genetic effects of naturally occurring flavonoids, anthraquinones and related compounds. Mutat. Res. 75, 243-277. https://doi.org/10.1016/0165-1110(80)90029-9
  3. Buckley-Sturrock A, Woodward S C, Senior R M, Griffin G L, Klagsbrun M, and Davidson J M (1989) Differential stimulation of collagenase and chemotatic activity in fibroblasts derived from rat wound repair tissue and human skin by growth factors. J. Cell. Physiol. 138, 70-78. https://doi.org/10.1002/jcp.1041380111
  4. Carones F, Vigo L, Scandola E, and Vacchini L (2002) Evaluation of the prophylactic use of mitomycin-C to inhibit haze formation after photorefractive keratectomy. J. Cataract Refract. Surg. 28, 2088-2095. https://doi.org/10.1016/S0886-3350(02)01701-7
  5. Chang S W (2004) Early corneal edema following topical application of mitomycin-C. J. Cataract Refract. Surg. 30, 1742-1750. https://doi.org/10.1016/j.jcrs.2003.12.044
  6. Cho J W, Cho S Y, Lee S R, and Lee K S (2010) Onion extract and quercetin induce matrix metalloproteinase-1 in vitro and in vivo. Int. J. Mol. Med. 25, 347-352.
  7. Crooke S T and Bradner W T (1976) Mytomycin C : a review. Cancer Treat. Rev. 3, 121-139. https://doi.org/10.1016/S0305-7372(76)80019-9
  8. Crowston J G, Wang X Y, Khaw P T, Zoellner H, and Healey P R (2006) Human serum reduces mitomycin-C cytotoxicity in human tenon's fibroblasts. Invest. Ophthalmol. Vis. Sci. 47, 946-952. https://doi.org/10.1167/iovs.05-1048
  9. Escariao A C, Nagasaki T, Zhao J, and Braunstein R (2008) Effects of mitomycin C on infiltration of polymorphonuclear leukocytes after epithelial scrape injury in the mouse cornea. Arq. Bras. Oftalmol. 71, 822-826. https://doi.org/10.1590/S0004-27492008000600011
  10. Formica J V and Regelson W (1995) Review of the biology of quercetin and related bioflavonoids. Food Chem. Toxicol. 33, 1061-1080. https://doi.org/10.1016/0278-6915(95)00077-1
  11. Fujitani A, Hayasaka S, Shibuya Y, and Noda S (1993) Corneoscleral ulceration and corneal perforation after pterygium excision and topical mitomycin C therapy. Ophthalmologica 207, 162-164. https://doi.org/10.1159/000310424
  12. Guardia T, Rotelli A E, Juarez A O, and Pelzer L E (2001) Anti-inflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Farmaco 56, 683-687. https://doi.org/10.1016/S0014-827X(01)01111-9
  13. Hashemi H, Taheri S M, Fotouhi A, and Kheiltash A (2004) Evaluation of the prophylactic use of mitomycin-C to inhibit haze formation after photorefractive keratectomy in high myopia: a prospective clinical study. BMC Ophthalmol. 4, 12. https://doi.org/10.1186/1471-2415-4-12
  14. Hertog M G, Hollman P C, Katan M B, and Kromhout D (1993) Intake of potentially anticarcinogenic flavonoids and their determinants in adults in The Netherlands. Nutr. Cancer 20, 21-29. https://doi.org/10.1080/01635589309514267
  15. Jampel H D (1992) Effect of brief exposure to mitomycin C on viability and proliferation of cultured human Tenon's capsule fibroblasts. Ophthalmology 99, 1471-1476. https://doi.org/10.1016/S0161-6420(92)31781-6
  16. Khaw P T, Sherwood M B, MacKay S L, Rossi M J, and Schultz G (1992) Five-minute treatments with fluorouracil, floxuridine, and mitomycin have long-term effects on human Tenon's capsule fibroblasts. Arch. Ophthalmol. 110, 1150-1154. https://doi.org/10.1001/archopht.1992.01080200130040
  17. Kim K S and Bae S H (2000) The effect of mitomycin-C on the function of corneal endothelial cells in rabbit. Korean J. Ophthalmol. 41, 1047-1053.
  18. Kim K S, Lee S Y, and Kim J H (2002) The inhibitory effects of mitomycin-C on the development of proliferative vitreoretinopathy. J. Korean Soc. 43, 204-212.
  19. Kim S J, Lee M H, Kim E H, and Lee J S (2008) Corneal opacity caused by LASEK with improper high-concentrated mitomycin-C. J. Korean Ophthalmol. Soc. 49, 1525-1531. https://doi.org/10.3341/jkos.2008.49.9.1525
  20. Kim Y T and Chung W S (2002) The effect of mitomycin C in endonasal dacryocystorhinostomy. J. Korean Ophthalmol. Soc. 43, 728-732.
  21. Kuhnau J (1976) The flavonoids: a class of semi-essential food components: their role in human nutrition. World Rev. Nutr. Diet. 24, 117-191. https://doi.org/10.1159/000399407
  22. Lisi S, Botta R, Lemmi M, Sellari-Franceschini S, Altea M A, Sisti E, Casini G, Nardi M, Marcocci C, Pinchera A, and Marino M (2010) Quercetin decreases proliferation of orbital fibroblasts and their release of hyaluronic acid. J. Endocrinol. Invest. 34, 521-527.
  23. Majmudar P A, Forstot S L, Dennis R F, Nirankari V S, Damiano R E, Brenart R, and Epstein R J (2000) Topical mitomycin-C for subepithelial fibrosis after refractive corneal surgery. Ophthalmology 107, 89-94. https://doi.org/10.1016/S0161-6420(99)00019-6
  24. Natto M V, Mohan R R, Ambrosio R Jr, Hutcheon A E, Zieske J D, and Wilson S E (2005) Wound healing in the cornea: a review of refractive surgery complications and new prospects for therapy. Cornea 24, 509-522. https://doi.org/10.1097/01.ico.0000151544.23360.17
  25. Reiterer G, Toborek M, and Hennig B (2004) Quercetin protects against linoleic acid-induced porcine endothelial cell dysfunction. J. Nutr. 134, 771-775.
  26. Rubinfeld R S, Pfister R R, Stein R M, Foster C S, Martin N F, Stoleru S, Talley A R, and Speaker M G (1992) Serious complications of topical mitomycin-C after pterygium surgery. Ophthalmology 99, 1647-1654. https://doi.org/10.1016/S0161-6420(92)31749-X
  27. Smith S, D'Amore P A, and Dreyer E B (1994) Comparative toxicity of mitomycin C and 5-fluorouracil in vitro. Am. J. Ophthalmol. 118, 332-337. https://doi.org/10.1016/S0002-9394(14)72957-5
  28. Xu H, Liu S, Xia X, Huang P, Wang P, and Wu X (2001) Mitomycin C reduces haze formation in rabbits after excimer laser photorefractive keratectomy. J. Refract. Surg. 17, 342-349.
  29. Zyzak L L, MacDonald L M, Batova A, Forand R, Creek K E, and Pirisi L (1994) Increased levels and constitutive tyrosine phosphorylation of the epidermal growth factor receptor contribute to autonomous growth of human papillomavirus type immortalized human keratinocytes. Cell Growth Differ. 5, 537-547.