Nutrition Research and Practice

  • 제8권5호
  • /
  • Pages.526-532
  • /
  • 2014
  • /
  • 1976-1457(pISSN)
  • /
  • 2005-6168(eISSN)
한국영양학회 (The Korean Nutrition Society)
권호 표지
DOI QR코드

DOI QR Code

Acanthopanax sessiliflorus stem confers increased resistance to environmental stresses and lifespan extension in Caenorhabditis elegans

  • Park, Jin-Kook (Department of Medical Biotechnology, College of Medical Science, Soonchunhyang University) ;
  • Kim, Chul-Kyu (Department of Medical Biotechnology, College of Medical Science, Soonchunhyang University) ;
  • Gong, Sang-Ki (Department of Medical Biotechnology, College of Medical Science, Soonchunhyang University) ;
  • Yu, A-Reum (Department of Medical Biotechnology, College of Medical Science, Soonchunhyang University) ;
  • Lee, Mi-Young (Department of Medical Biotechnology, College of Medical Science, Soonchunhyang University) ;
  • Park, Sang-Kyu (Department of Medical Biotechnology, College of Medical Science, Soonchunhyang University)
  • 투고 : 2013.11.21
  • 심사 : 2014.08.12
  • 발행 : 2014.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

BACKGROUND/OBJECTIVES: Acanthopanax sessiliflorus is a native Korean plant and used as a traditional medicine or an ingredient in many Korean foods. The free radical theory of aging suggests that cellular oxidative stress caused by free radicals is the main cause of aging. Free radicals can be removed by cellular anti-oxidants. MATERIALS/METHODS: Here, we examined the anti-oxidant activity of Acanthopanax sessiliflorus extract both in vitro and in vivo. Survival of nematode C. elegans under stress conditions was also compared between control and Acanthopanax sessiliflorus extract-treated groups. Then, anti-aging effect of Acanthopanax sessiliflorus extract was monitored in C. elegans. RESULTS: Stem extract significantly reduced oxidative DNA damage in lymphocyte, which was not observed by leaves or root extract. Survival of C. elegans under oxidative-stress conditions was significantly enhanced by Acanthopanax sessiliflorus stem extract. In addition, Acanthopanax sessiliflorus stem increased resistance to other environmental stresses, including heat shock and ultraviolet irradiation. Treatment with Acanthopanax sessiliflorus stem extract significantly extended both mean and maximum lifespan in C. elegans. However, fertility was not affected by Acanthopanax sessiliflorus stem. CONCLUSION: Different parts of Acanthopanax sessiliflorus have different bioactivities and stem extract have strong anti-oxidant activity in both rat lymphocytes and C. elegans, and conferred a longevity phenotype without reduced reproduction in C. elegans, which provides conclusive evidence to support the free radical theory of aging.

키워드

참고문헌

  1. Harman D. The free radical theory of aging. Antioxid Redox Signal 2003;5:557-61. https://doi.org/10.1089/152308603770310202
  2. Harman D. Aging: a theory based on free radical and radiation chemistry. J Gerontol 1956;11:298-300. https://doi.org/10.1093/geronj/11.3.298
  3. Beckman KB, Ames BN. The free radical theory of aging matures. Physiol Rev 1998;78:547-81. https://doi.org/10.1152/physrev.1998.78.2.547
  4. Powolny AA, Singh SV, Melov S, Hubbard A, Fisher AL. The garlic constituent diallyl trisulfide increases the lifespan of C. elegans via skn-1 activation. Exp Gerontol 2011;46:441-52. https://doi.org/10.1016/j.exger.2011.01.005
  5. Kitani K, Yokozawa T, Osawa T. Interventions in aging and ageassociated pathologies by means of nutritional approaches. Ann N Y Acad Sci 2004;1019:424-6. https://doi.org/10.1196/annals.1297.075
  6. Martorell P, Bataller E, Llopis S, Gonzalez N, Alvarez B, Monton F, Ortiz P, Ramon D, Genoves S. A cocoa peptide protects Caenorhabditis elegans from oxidative stress and ${\beta}$-amyloid peptide toxicity. PLoS One 2013;8:e63283. https://doi.org/10.1371/journal.pone.0063283
  7. Park SK, Page GP, Kim K, Allison DB, Meydani M, Weindruch R, Prolla TA. alpha- and gamma-Tocopherol prevent age-related transcriptional alterations in the heart and brain of mice. J Nutr 2008;138:1010-8. https://doi.org/10.1093/jn/138.6.1010
  8. Park SK, Kim K, Page GP, Allison DB, Weindruch R, Prolla TA. Gene expression profiling of aging in multiple mouse strains: identification of aging biomarkers and impact of dietary antioxidants. Aging Cell 2009;8:484-95. https://doi.org/10.1111/j.1474-9726.2009.00496.x
  9. Park SK, Kim JJ, Yu AR, Lee MY, Park SK. Electrolyzed-reduced water confers increased resistance to environmental stresses. Mol Cell Toxicol 2012;8:241-7. https://doi.org/10.1007/s13273-012-0029-1
  10. Park SK, Park SK. Electrolyzed-reduced water increases resistance to oxidative stress, fertility, and lifespan via insulin/IGF-1-like signal in C. elegans. Biol Res 2013;46:147-52. https://doi.org/10.4067/S0716-97602013000200005
  11. Jung BS, Shin MK. Hyang Yak Dae Sa Jeon. 3rd ed. Seoul: Young Lim Sa Publisher; 2003.
  12. Fujikawa T, Yamaguchi A, Morita I, Takeda H, Nishibe S. Protective effects of Acanthopanax senticosus Harms from Hokkaido and its components on gastric ulcer in restrained cold water stressed rats. Biol Pharm Bull 1996;19:1227-30. https://doi.org/10.1248/bpb.19.1227
  13. Bae EA, Yook CS, Oh OJ, Chang SY, Nohara T, Kim DH. Metabolism of chiisanoside from Acanthopanax divaricatus var. albeofructus by human intestinal bacteria and its relation to some biological activities. Biol Pharm Bull 2001;24:582-5. https://doi.org/10.1248/bpb.24.582
  14. Jung HJ, Nam JH, Choi J, Lee KT, Park HJ. Antiinflammatory effects of chiisanoside and chiisanogenin obtained from the leaves of Acanthopanax chiisanensis in the carrageenan- and Freund's complete adjuvant-induced rats. J Ethnopharmacol 2005;97:359-67. https://doi.org/10.1016/j.jep.2004.11.026
  15. Kim YO, Cho DH, Chung HJ, Kim JH, Chang SY, Yook CS, Yang KS, Oh OJ. Effects of lupane-triterpenoids on mitogen-induced proliferation of lymphocytes. Yakhak Hoeji 1999;43:208-13.
  16. Jeong SC, Jeong YT, Yang BK, Song CH. Chemical characteristics and immuno-stimulating properties of biopolymers extracted from Acanthopanax sessiliflorus. J Biochem Mol Biol 2006;39:84-90. https://doi.org/10.5483/BMBRep.2006.39.1.084
  17. Jeong SC, Yang BK, Jeong YT, Rao KS, Song CH. Isolation and characterization of biopolymers extracted from the bark of Acanthopanax sessiliflorus and their anticomplement activity. J Microbiol Biotechnol 2007;17:21-8.
  18. Yoshizumi K, Hirano K, Ando H, Hirai Y, Ida Y, Tsuji T, Tanaka T, Satouchi K, Terao J. Lupane-type saponins from leaves of Acanthopanax sessiliflorus and their inhibitory activity on pancreatic lipase. J Agric Food Chem 2006;54:335-41. https://doi.org/10.1021/jf052047f
  19. Ryu AR, Kim JH, Lee MY. Suppressive effect of Acanthopanax sessiliflorus extract on the DNA and cell damage by Dieldrin. Korean J Med Crop Sci 2012;20:245-50. https://doi.org/10.7783/KJMCS.2012.20.4.245
  20. Kim HY, Kim K. Protein glycation inhibitory and antioxidative activities of some plant extracts in vitro. J Agric Food Chem 2003;51:1586-91. https://doi.org/10.1021/jf020850t
  21. Wiegant FA, Surinova S, Ytsma E, Langelaar-Makkinje M, Wikman G, Post JA. Plant adaptogens increase lifespan and stress resistance in C. elegans. Biogerontology 2009;10:27-42. https://doi.org/10.1007/s10522-008-9151-9
  22. Kim CK, Park SK. Effect of Acanthopanax sessiliflorus extracts on stress response and aging in Caenorhabditis elegans. Food Sci Technol Res 2013;19:439-44. https://doi.org/10.3136/fstr.19.439
  23. Liu SM, Li XZ, Huo Y, Lu F. Protective effect of extract of Acanthopanax senticosus Harms on dopaminergic neurons in Parkinson's disease mice. Phytomedicine 2012;19:631-8. https://doi.org/10.1016/j.phymed.2012.02.006
  24. Park SK, Lee MY. Suppressive effects of various antioxidants on melamine-induced oxidative DNA damage in human lymphocytes. Mol Cell Toxicol 2009;5:243-9.
  25. Peto R, Peto J. Asymptotically efficient rank invarient test procedures. J R Stat Soc Ser A Stat Soc 1972;135:185-207. https://doi.org/10.2307/2344317
  26. Treinin M, Shliar J, Jiang H, Powell-Coffman JA, Bromberg Z, Horowitz M. HIF-1 is required for heat acclimation in the nematode Caenorhabditis elegans. Physiol Genomics 2003;14:17-24. https://doi.org/10.1152/physiolgenomics.00179.2002
  27. Sohal RS, Weindruch R. Oxidative stress, caloric restriction, and aging. Science 1996;273:59-63. https://doi.org/10.1126/science.273.5271.59
  28. Hughes SE, Evason K, Xiong C, Kornfeld K. Genetic and pharmacological factors that influence reproductive aging in nematodes. PLoS Genet 2007;3:e25. https://doi.org/10.1371/journal.pgen.0030025
  29. Larsen PL, Albert PS, Riddle DL. Genes that regulate both development and longevity in Caenorhabditis elegans. Genetics 1995;139: 1567-83.
  30. Gems D, Sutton AJ, Sundermeyer ML, Albert PS, King KV, Edgley ML, Larsen PL, Riddle DL. Two pleiotropic classes of daf-2 mutation affect larval arrest, adult behavior, reproduction and longevity in Caenorhabditis elegans. Genetics 1998;150:129-55.
  31. Yook CS, Rho YS, Seo SH, Leem JY, Han DR. Chemical components of Acanthopanax divaricatus and anticancer effect in leaves. Yakhak Hoeji 1996;40:251-61.
  32. Hahn DR, Kim CJ, Kim JH. A study on the chemical constituents of Acanthopanax koreanum Nakai and its pharmaco-biological activities. Yakhak Hoeji 1985;29:357-61.
  33. Fu J, Fu J, Yuan J, Zhang N, Gao B, Fu G, Tu Y, Zhang Y. Anti-diabetic activities of Acanthopanax senticosus polysaccharide (ASP) in combination with metformin. Int J Biol Macromol 2012;50:619-23. https://doi.org/10.1016/j.ijbiomac.2012.01.034
  34. Liu XZ, Yan D. Ageing and hearing loss. J Pathol 2007;211:188-97. https://doi.org/10.1002/path.2102
  35. Lee CK, Weindruch R, Prolla TA. Gene-expression profile of the ageing brain in mice. Nat Genet 2000;25:294-7. https://doi.org/10.1038/77046
  36. Suthammarak W, Somerlot BH, Opheim E, Sedensky M, Morgan PG. Novel interactions between mitochondrial superoxide dismutases and the electron transport chain. Aging Cell 2013;12:1132-40. https://doi.org/10.1111/acel.12144
  37. Lagouge M, Larsson NG. The role of mitochondrial DNA mutations and free radicals in disease and ageing. J Intern Med 2013;273: 529-43. https://doi.org/10.1111/joim.12055
  38. Ivanova DG, Yankova TM. The free radical theory of aging in search of a strategy for increasing life span. Folia Med (Plovdiv) 2013; 55:33-41.
  39. Goto S. Evidence for and against anti-aging effects based on model animal studies. Nihon Rinsho 2009;67:1337-40.
  40. Mecocci P, Polidori MC, Troiano L, Cherubini A, Cecchetti R, Pini G, Straatman M, Monti D, Stahl W, Sies H, Franceschi C, Senin U. Plasma antioxidants and longevity: a study on healthy centenarians. Free Radic Biol Med 2000;28:1243-8. https://doi.org/10.1016/S0891-5849(00)00246-X
  41. Lipman RD, Bronson RT, Wu D, Smith DE, Prior R, Cao G, Han SN, Martin KR, Meydani SN, Meydani M. Disease incidence and longevity are unaltered by dietary antioxidant supplementation initiated during middle age in C57BL/6 mice. Mech Ageing Dev 1998;103: 269-84. https://doi.org/10.1016/S0047-6374(98)00048-7
  42. Navarro A, Gomez C, Sanchez-Pino MJ, Gonzalez H, Bandez MJ, Boveris AD, Boveris A. Vitamin E at high doses improves survival, neurological performance, and brain mitochondrial function in aging male mice. Am J Physiol Regul Integr Comp Physiol 2005; 289:R1392-9. https://doi.org/10.1152/ajpregu.00834.2004
  43. Bass TM, Weinkove D, Houthoofd K, Gems D, Partridge L. Effects of resveratrol on lifespan in Drosophila melanogaster and Caenorhabditis elegans. Mech Ageing Dev 2007;128:546-52. https://doi.org/10.1016/j.mad.2007.07.007
  44. Agarwal B, Baur JA. Resveratrol and life extension. Ann N Y Acad Sci 2011;1215:138-43. https://doi.org/10.1111/j.1749-6632.2010.05850.x
  45. Kirkwood TB. Evolution of ageing. Nature 1977;270:301-4. https://doi.org/10.1038/270301a0
  46. Hsin H, Kenyon C. Signals from the reproductive system regulate the lifespan of C. elegans. Nature 1999;399:362-6. https://doi.org/10.1038/20694

피인용 문헌

  1. Role of Coelomocytes in Stress Response and Fertility in Caenorhabditis elegans vol.25, pp.3, 2015, https://doi.org/10.5352/JLS.2015.25.3.263
  2. vol.19, pp.10, 2016, https://doi.org/10.1089/jmf.2016.3729
  3. Current Perspective in the Discovery of Anti-aging Agents from Natural Products vol.7, pp.5, 2017, https://doi.org/10.1007/s13659-017-0135-9
  4. vol.82, pp.6, 2017, https://doi.org/10.1111/1750-3841.13720
  5. Coelomocytes are required for lifespan extension via different methods of dietary restriction in C. elegans vol.9, pp.1, 2017, https://doi.org/10.1007/s13530-017-0304-6
  6. Artemisia annua increases resistance to heat and oxidative stresses, but has no effect on lifespan in Caenorhabditis elegans vol.36, pp.2, 2016, https://doi.org/10.1590/1678-457X.0115
  7. Selenocysteine modulates resistance to environmental stress and confers anti-aging effects in C. elegans vol.72, pp.8, 2014, https://doi.org/10.6061/clinics/2017(08)07
  8. Phosphatidylcholine Extends Lifespan via DAF-16 and Reduces Amyloid-Beta-Induced Toxicity in Caenorhabditis elegans vol.2019, pp.None, 2014, https://doi.org/10.1155/2019/2860642
  9. The Impact of Plant Bioactive Compounds on Aging and Fertility of Diverse Organisms: A Review vol.20, pp.13, 2014, https://doi.org/10.2174/1389557520666200429101942
  10. Effects of ethyl acetate fractional extract from Portulaca oleracea L. (PO‐EA) on lifespan and healthspan in Caenorhabditis elegans vol.85, pp.12, 2014, https://doi.org/10.1111/1750-3841.15507
  11. Eleutherococcus sessiliflorus Inhibits Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL)-Induced Osteoclast Differentiation and Prevents Ovariectomy (OVX)-Induced Bone Loss vol.26, pp.7, 2021, https://doi.org/10.3390/molecules26071886
  12. Noble 3,4-Seco-triterpenoid Glycosides from the Fruits of Acanthopanax sessiliflorus and Their Anti-Neuroinflammatory Effects vol.10, pp.9, 2014, https://doi.org/10.3390/antiox10091334