International Journal of Oral Biology

  • 제39권3호
  • /
  • Pages.145-151
  • /
  • 2014
  • /
  • 1226-7155(pISSN)
  • /
  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
권호 표지
DOI QR코드

DOI QR Code

Lactoferrin Constitutively Enhances Differentiation of Osteoblastic MC3T3-E1 Cells in Vitro

  • Yang, Hee-Young (Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University) ;
  • Lee, Ha-Mi (Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Park, Byung-Ju (Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University) ;
  • Lee, Tae-Hoon (Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University)
  • 투고 : 2014.08.05
  • 심사 : 2014.09.05
  • 발행 : 2014.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

During bone remodeling, there is requirement of differentiation of osteoblastic cells. Previously, we identified proteins differentially expressed in soft tissue during bone healing. Of these proteins, we focused the effect of LTF on differentiation of osteoblast. In order to analyze the osteogenic ability of LTF, we treated conditioned media collected from human LTF-stably transfected HEK293T cells into osteoblastic MC3T3-E1. The results showed that the activity and expression of alkaline phosphatase were increased in MC3T3-E1 cells treated with conditioned media containing LTF in dose- and time-dependent manner. At the same time, we observed the significant increase of the expression of osteoblastic genes, such as ALP, BSP, COL1A1, and OCN, and along with matrix mineralization genes, such as DMP1 and DMP2, in LTF conditioned media-treated groups. Moreover, the result of treating recombinant human LTF directly into osteoblastic MC3T3-E1 showed the same pattern of treating conditioned media containing LTF. Our study demonstrated that LTF constitutively enhances osteoblastic differentiation via induction of osteoblastic genes and activation of matrix mineralization in MC3T3-E1 cells.

키워드

참고문헌

  1. Zuo C, Huang Y, Bajis R, Sahih M, Li YP, Dai K, Zhang X. Osteoblastogenesis regulation signals in bone remodeling. Osteoporos Int. 2012;23:1653-1663. https://doi.org/10.1007/s00198-012-1909-x
  2. Hadjidakis DJ, Androulakis, II. Bone remodeling. Ann N Y Acad Sci. 2006;1092:385-396. https://doi.org/10.1196/annals.1365.035
  3. Ward PP, Paz E, Conneely OM. Multifunctional roles of lactoferrin: a critical overview. Cell Mol Life Sci. 2005;62:2540-2548. https://doi.org/10.1007/s00018-005-5369-8
  4. Teng CT. Lactoferrin gene expression and regulation: an overview. Biochem Cell Biol. 2002;80:7-16. https://doi.org/10.1139/o01-215
  5. Oh SM, Hahm DH, Kim IH, Choi SY. Human neutrophil lactoferrin trans-activates the matrix metalloproteinase 1 gene through stress-activated MAPK signaling modules. J Biol Chem. 2001;276:42575-42579. https://doi.org/10.1074/jbc.M107724200
  6. Grey A, Banovic T, Zhu Q, Watson M, Callon K, Palmano K, Ross J, Naot D, Reid IR, Cornish J. The low-density lipoprotein receptor-related protein 1 is a mitogenic receptor for lactoferrin in osteoblastic cells. Mol Endocrinol. 2004;18:2268-2278. https://doi.org/10.1210/me.2003-0456
  7. Oh SM, Pyo CW, Kim Y, Choi SY. Neutrophil lactoferrin upregulates the human p53 gene through induction of NF-kappaB activation cascade. Oncogene. 2004;23:8282-8291. https://doi.org/10.1038/sj.onc.1208021
  8. Guo HY, Jiang L, Ibrahim SA, Zhang L, Zhang H, Zhang M, Ren FZ. Orally administered lactoferrin preserves bone mass and microarchitecture in ovariectomized rats. J Nutr. 2009;139:958-964. https://doi.org/10.3945/jn.108.100586
  9. Yamano E, Miyauchi M, Furusyo H, Kawazoe A, Ishikado A, Makino T, Tanne K, Tanaka E, Takata T. Inhibitory effects of orally administrated liposomal bovine lactoferrin on the LPS-induced osteoclastogenesis. Lab Invest. 2010;90:1236-1246. https://doi.org/10.1038/labinvest.2010.80
  10. Du M, Xu W, Yi H, Han X, Wang C, Zhang L. Protective effects of bovine colostrum acid proteins on bone loss of ovariectomized rats and the ingredients identification. Mol Nutr Food Res. 2011;55:220-228. https://doi.org/10.1002/mnfr.200900593
  11. Takayama Y, Mizumachi K. Effect of lactoferrin-embedded collagen membrane on osteogenic differentiation of human osteoblast-like cells. J Biosci Bioeng. 2009;107:191-195. https://doi.org/10.1016/j.jbiosc.2008.09.018
  12. Yang HY, Kwon J, Kook MS, Kang SS, Kim SE, Sohn S, Jung S, Kwon SO, Kim HS, Lee JH, Lee TH. Proteomic analysis of gingival tissue and alveolar bone during alveolar bone healing. Mol Cell Proteomics. 2013;12:2674-2688. https://doi.org/10.1074/mcp.M112.026740
  13. Naot D, Grey A, Reid IR, Cornish J. Lactoferrin-a novel bone growth factor. Clin Med Res. 2005;3:93-101. https://doi.org/10.3121/cmr.3.2.93
  14. Cornish J, Callon KE, Naot D, Palmano KP, Banovic T, Bava U, Watson M, Lin JM, Tong PC, Chen Q, Chan VA, Reid HE, Fazzalari N, Baker HM, Baker EN, Haggarty NW, Grey AB, Reid IR. Lactoferrin is a potent regulator of bone cell activity and increases bone formation in vivo. Endocrinology. 2004;145:4366-4374. https://doi.org/10.1210/en.2003-1307
  15. Khosla S, Riggs BL. Pathophysiology of age-related bone loss and osteoporosis. Endocrinol Metab Clin North Am. 2005;34:1015-1030. https://doi.org/10.1016/j.ecl.2005.07.009
  16. Marie PJ, Kassem M. Osteoblasts in osteoporosis: past, emerging, and future anabolic targets. Eur J Endocrinol. 2011;165:1-10. https://doi.org/10.1530/EJE-11-0132
  17. Riggs BL, Parfitt AM. Drugs used to treat osteoporosis: the critical need for a uniform nomenclature based on their action on bone remodeling. J Bone Miner Res. 2005;20: 177-184.