International Journal of Oral Biology

  • 제39권3호
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  • Pages.131-136
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  • 2014
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Clathrin and Lipid Raft-dependent Internalization of Porphyromonas gingivalis in Endothelial Cells

  • Kim, Sang-Yong (Department of Oral Microbiology, School of Dentistry, Chonnam National University) ;
  • Kim, So-Hee (Department of Oral Microbiology, School of Dentistry, Chonnam National University) ;
  • Choi, Eun-Kyoung (Department of Oral Microbiology, School of Dentistry, Chonnam National University) ;
  • Paek, Yun-Woong (Department of Physical Therapy, Gwangju Health University) ;
  • Kang, In-Chol (Department of Oral Microbiology, School of Dentistry, Chonnam National University)
  • 투고 : 2014.06.03
  • 심사 : 2014.08.28
  • 발행 : 2014.09.30
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초록

Porphyromonas gingivalis is one of the most important periodontal pathogens and has been to known to invade various types of cells, including endothelial cells. The present study investigated the mechanisms involved in the internalization of P. gingivalis in human umbilical vein endothelial cells (HUVEC). P. gingivalis internalization was reduced by clathrin and lipid raft inhibitors, as well as a siRNA knockdown of caveolin-1, a principal molecule of lipid raft-related caveolae. The internalization was also reduced by perturbation of actin rearrangement, while microtubule polymerization was not required. Furthermore, we found that Src kinases are critical for the internalization of P. gingivalis into HUVEC, while neither Rho family GTPases nor phosphatidylinositol 3-kinase are required. Taken together, this study indicated that P. gingivalis internalization into endothelial cells involves clathrin and lipid rafts and requires actin rearrangement associated with Src kinase activation.

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참고문헌

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