Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Pyunkang-hwan (Pyunkang-tang) Regulates Hypersecretion of Pulmonary Mucin from Rats with Sulfur Dioxide-Induced Bronchitis and Production and Gene Expression of MUC5AC Mucin from Human Airway Epithelial Cells

  • Seo, Hyo-Seok (Department of Pharmacology, School of Medicine, Chungnam National University) ;
  • Lee, Hyun Jae (Department of Pharmacology, School of Medicine, Chungnam National University) ;
  • Lee, Choong Jae (Department of Pharmacology, School of Medicine, Chungnam National University)
  • Received : 2014.05.25
  • Accepted : 2014.06.25
  • Published : 2014.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Pyunkang-hwan (Pyunkang-tang) extract (PGT) is a traditional folk medicine for controlling diverse pulmonary diseases including bronchitis, tonsiltis and pneumonitis. We investigated whether PGT significantly affects secretion, production and gene expression of airway mucin using in vivo and in vitro experimental models reflecting the hypersecretion and/or hyperproduction of mucus observed in inflammatory pulmonary diseases. For in vivo experiment, effect of PGT was checked on hypersecretion of pulmonary mucin in sulfur dioxide-induced bronchitis in rats. For in vitro experiment, confluent NCI-H292 cells were pretreated with PGT for 30 min and then stimulated with EGF (epidermal growth factor), PMA (phorbol 12-myristate 13-acetate) or TNF-${\alpha}$ (tumor necrosis factor-${\alpha}$) for 24 h. The MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. The results were as follows: (1) PGT inhibited the expression of MUC5AC mucin gene induced by EGF, PMA or TNF-${\alpha}$ from NCI-H292 cells, respectively; (2) PGT also inhibited the production of MUC5AC mucin protein induced by the same inducers from NCI-H292 cells, respectively; (3) PGT inhibited secretion of mucin in sulfur dioxide-induced bronchitis rat model. This result suggests that PGT can regulate secretion, production and gene expression of airway mucin.

Keywords

References

  1. Voynow, J. A.; Rubin, B. K. Chest. 2009, 135(2), 505-512. https://doi.org/10.1378/chest.08-0412
  2. Heo, H. J.; Lee, S. Y.; Lee, M. N.; Lee, H. J.; Seok, J. H.; Lee, C. J. Phytother. Res. 2009, 23, 1458-1461. https://doi.org/10.1002/ptr.2801
  3. Lee, H. J.; Lee, S. Y.; Bae, H. S.; Kim, J. H.; Chang, G. T.; Seok, J. H.; Lee, C. J. Phytomedicine. 2011, 18, 743-747. https://doi.org/10.1016/j.phymed.2010.11.003
  4. Lee, H. J.; Lee, S. Y.; Lee, M. N.; Kim, J. H.; Chang, G. T.; Seok, J. H.; Lee, C. J. Pulm. Pharmacol. Ther. 2011, 24, 128-132. https://doi.org/10.1016/j.pupt.2010.08.003
  5. Lee, H. J.; Lee, S. Y.; Lee, M. N.; Kim, J. H.; Chang, G. T.; Seok, J. H.; Lee, C. J. Phytother. Res. 2011, 25, 1196-1200. https://doi.org/10.1002/ptr.3362
  6. Jang, I. M.; Treatise on asian herbal medicines. Haksul-pyunsukwan in Research institute of natural products of Seoul National University, Seoul, 2003; p 1438.
  7. Li, J. D.; Dohrman, A. F.; Gallup, M.; Miyata, S.; Gum, J. R.; Kim, Y. S.; Nadel, J. A.; Prince, A.; Basbaum, C.B. Proc. Natl. Acad. Sci. USA. 1997, 94, 967-972. https://doi.org/10.1073/pnas.94.3.967
  8. Takeyama, K.; Dabbagh, K.; Lee, H. M.; Agusti, C.; Lausier, J. A.; Ueki, I. F.; Grattan, K. M.; Nadel, J. A. Proc. Natl. Acad. Sci. USA. 1999, 96, 3081-3086. https://doi.org/10.1073/pnas.96.6.3081
  9. Shao, M. X.; Ueki, I. F.; Nadel, J. A. Proc. Natl. Acad. Sci. USA. 2003, 100, 11618-11623. https://doi.org/10.1073/pnas.1534804100
  10. Rogers, D. F.; Barnes, P. J. Ann. Med. 2006, 38(2), 116-125. https://doi.org/10.1080/07853890600585795
  11. Fischer, B. M.; Rochelle, L. G.; Voynow, J. A.; Akley, N. J.; Adler K. B. Am. J. Respir. Cell. Mol. Biol. 1999, 20, 413-422. https://doi.org/10.1165/ajrcmb.20.3.3393
  12. Song, K.; Lee, W. J.; Chung, K. C.; Koo, J. S.; Yang, E. J.; Choi, J. Y.; Yoon, J. H. J. Biol. Chem. 2003, 278, 23243-23250. https://doi.org/10.1074/jbc.M300096200
  13. Cohn, L.; Whittaker, L.; Niu, N.; Homer, R. J. Cytokine regulation of mucus production in a model of allergic asthma. Novartis Found. Symp. 2002; 248, 201-213.
  14. Takeyama, K.; Dabbagh, K.; Shim, J. J.; Dao-Pick, T.; Ueki, I. F.; Nadel, J. A. J. Immunol. 2000, 164, 1546-1552. https://doi.org/10.4049/jimmunol.164.3.1546
  15. Hong, D. H.; Petrovics, G.; Anderson, W. B.; Forstner, J.; Forstner, G. Am. J. Physiol. 1999, 277, G1041-G1047.
  16. Hewson, C. A.; Edbrooke, M. R.; Johnston, S. L. J. Mol. Biol. 2004, 344, 683-695. https://doi.org/10.1016/j.jmb.2004.09.059
  17. Park, S. J.; Kang, S. Y.; Kim, N. S.; Kim, H. M. Immunopharmacol. Immunotoxicol. 2002, 24(2), 211-226. https://doi.org/10.1081/IPH-120003751