DOI QR코드

DOI QR Code

Suppression of Eimeria tenella Sporulation by Disinfectants

  • You, Myung-Jo (Laboratory of Veterinary Parasitology, College of Veterinary Medicine and Biosafety Research Center, Chonbuk National University)
  • Received : 2013.12.24
  • Accepted : 2014.06.24
  • Published : 2014.08.31

Abstract

The disinfectant effects (DEs) of 10 types of chemicals, defined by their ability to destroy or inhibit oocysts and consequently prevent sporulation of Eimeria tenella field isolate, were evaluated in vitro. Correct species assignments and sample purities were confirmed by the singular internal transcribed spacer (ITS)-PCR analysis. A total of 18 treatments were performed, and the disinfection suppression levels were 75.9% for 39% benzene + 22% xylene (1:10 dilution), 85.5% for 30% cresol soup (1:1 dilution), and 91.7% for 99.9% acetic acid (1:2 dilution) group. The results indicate that acetic acid, cresol soup, and benzene+xylene are good candidates for suppression of E. tenella oocyst sporulation.

Keywords

References

  1. Daugschies A, Bose R, Marx J, Teich K, Friedhoff KT. Development and application of a standardized assay for chemical disinfection of coccidia oocysts. Vet Parasitol 2002; 103: 299-308. https://doi.org/10.1016/S0304-4017(01)00581-7
  2. McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev 1999; 12: 147-179.
  3. Williams RB. Laboratory tests of phenolic disinfectants as oocysticides against the chicken coccidium Eimeria tenella. Veterinary Record 1997; 141: 447-448. https://doi.org/10.1136/vr.141.17.447
  4. Rehman TU, Khan MN, Sajid MS, Abbas RZ, Arshad M, Iqbal Z. Epidemiology of Eimeria and associated risk factors in cattle of district Toba Tek Singh, Pakistan. Parasitol Res 2011; 108: 1171-1177. https://doi.org/10.1007/s00436-010-2159-5
  5. Williams RB. Tracing the emergence of drug-resistance in coccidia (Eimeria spp.) of commercial broiler flocks medicated with decoquinate for the first time in the United Kingdom. Vet Parasitol 2006; 135: 1-14. https://doi.org/10.1016/j.vetpar.2005.10.012
  6. Haug A, Thebo P, Mattsson JG. A simplified protocol for molecular identification of Eimeria species in field samples. Vet Parasitol 2007; 146: 35-45. https://doi.org/10.1016/j.vetpar.2006.12.015
  7. Guimaraes JS, Bogado ALG, Da Cunha TCB, Garcia JL. In vitro evaluation of the disinfection efficacy on Eimeria tenella, unsporulated oocysts isolated from broilers. Rev Bras Parasitol Vet 2007; 16: 67-71.
  8. Hilbrich P. Disinfection tests on Eimeria tenella oocytes. Berl Munch Tierarztl Wochenschr 1975; 88: 144-148.
  9. Daugschies A, Agneessens J, Goossens L, Mengel H, Veys P. The effect of a metaphylactic treatment with diclazuril (Vecoxan$^{(R)}$) on the oocyst excretion and growth performance of calves exposed to a natural Eimeria infection. Veterinary Parasitology 2007; 149: 199-206. https://doi.org/10.1016/j.vetpar.2007.08.003
  10. Ayeni AO, Dingeldein E, Durr U. Studies on the inactivation of coccidian oocysts. Acta Vet Acad Sci Hung 1972; 22: 111-122.
  11. Grier N. Synthesis and antimicrobial evaluation of quaternary salts of 4-phenyl-1,2,3,6-tetrahydropyridine and 3,6-dimethyl-6-phenyl-tetrahydro-2H-1,3-oxazine. J Pharm Sci 1979; 68: 407-411. https://doi.org/10.1002/jps.2600680404
  12. Zaman MA, Iqbal Z, Abbas RZ, Khan MN. Anticoccidial activity of herbal complex in broiler chickens challenged with Eimeria tenella. Parasitology 2012; 139: 237-243. https://doi.org/10.1017/S003118201100182X

Cited by

  1. The Effect of Increased Temperatures on Viability, Morphology, Infectivity, and Development of Eimeria Tenella vol.106, pp.3, 2014, https://doi.org/10.1645/19-17
  2. Heat stress reduces sexual development and affects pathogenesis of Eimeria maxima in meat-type chickens vol.10, pp.None, 2020, https://doi.org/10.1038/s41598-020-67330-w