Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Molecular Characterization of Tomato Yellow Leaf Curl Virus in Korea and the Construction of an Infectious Clone

  • Lee, Bong Choon (Crop Environment Research Division, National Institute of Crop Science, Rural Developement Administration) ;
  • Ueda, Shigenori (NARO Hokkaido Agricultural Research Center) ;
  • Yoon, Young-Nam (Crop Environment Research Division, National Institute of Crop Science, Rural Developement Administration) ;
  • Shin, Dong Bum (Crop Environment Research Division, National Institute of Crop Science, Rural Developement Administration) ;
  • Kang, Hang-Won (Crop Environment Research Division, National Institute of Crop Science, Rural Developement Administration)
  • Received : 2014.06.02
  • Accepted : 2014.08.22
  • Published : 2014.09.30
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Abstract

Several tomato production regions in Korea were surveyed for tomato yellow leaf curl disease (TYLCD). Tomato leaf samples showing TYLCD-like symptoms were collected from Tongyeong (To), Geoje (Gi), and Gimhae (Gh) cities of the southern part of Korea. Tomato yellow leaf curl virus (TYLCV) was detected and the full-length genomes of the isolates were sequenced. The TYLCV isolates found in Korea shared high sequence identity (> 99%) with TYLCV-IL [JR:Omu:Ng] (AB110217). Phylogenetic relationship analysis revealed that they formed two groups (with little genetic variability), and the To, Gj, and Gh isolates belonged to the TYLCV-IL group. An infectious clone of TYLCV-To (JQ013089) was constructed and agroinoculated into Nicotiana benthamiana, Nicotiana tabacum var. Xanthi, Petunia hybrida, Capsicum annuum, and Lycopersicon esculentum cv. Hausumomotaro. Agroinfection with a dimeric infectious clone of TYLCV-To induced severe leaf curling and stunting symptoms in these plants, excluding C. annuum. Tomato plants then developed typical yellow leaf curl symptoms.

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References

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