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Rhizoctonia 및 Pythium 모잘록병에 대한 Serenade 제제의 방제효과

Control Efficacy of Serenade Formulation against Rhizoctonia and Pythium Damping-off Diseases

  • 조은주 (한국화학연구원 바이오화학연구센터) ;
  • 강범관 (바이엘크롭사이언스) ;
  • 장경수 (한국화학연구원 바이오화학연구센터) ;
  • 최용호 (한국화학연구원 바이오화학연구센터) ;
  • 김진철 (전남대학교 응용생물공학부) ;
  • 최경자 (한국화학연구원 바이오화학연구센터)
  • Jo, Eun Ju (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Kang, Bum Gwan (Bayer CropScience Ltd.) ;
  • Jang, Kyoung Soo (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong Ho (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Choi, Gyung Ja (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
  • 투고 : 2014.06.12
  • 심사 : 2014.07.01
  • 발행 : 2014.09.30

초록

Rhizoctonia solani와 Pythium ultimum에 의한 모잘록병은 대부분의 작물에서 전 세계적으로 발생하여 종자와 유묘에 심각한 피해를 주고 있다. Serenade 제제(1.34%, SC)의 Rhizoctonia 및 Pythium 모잘록병 방제 효과를 조사하기 위하여, 병원균 접종 토양에 파종된 고추와 오이에 Serenade 제형 9배, 19배 및 39배 희석액을 관주 처리하였다. Serenade 제제를 9배 및 19배 희석액으로 처리하였을 때, 고추의 Rhizoctonia 모잘록병에 대한 Serenade 제제의 방제효과는 각각 58%와 29%이었다. 그리고 오이에서도 고추의 Rhizoctonia 모잘록병과 유사한 각각 54%와 28%의 방제효과를 보였다. 반면에 오이 Pythium 모잘록병에 대한 Serenade 제형의 방제효과는 Rhizoctonia 모잘록병에 비하여 아주 낮은 방제효과를 나타내었고, Serenade 9배 희석액으로 처리한 고추의 Pythium 모잘록병에 대해서만 무처리구와 유의성 있는 발병도 차이를 보였다. 이상의 결과로부터 Serenade 제형은 Rhizoctonia 및 Pythium 모잘록병을 효과적으로 방제할 수 있을 것으로 생각되었다.

Damping-off, caused by Rhizoctonia solani and Pythium ultimum, is a very important plant disease and affects seeds and seedlings of many plant species worldwide. To investigate control efficacy of Serenade formulation (1.34%, SC) against Rhizoctonia and Pythium damping-off diseases, seeds of chili pepper and cucumber were sown in soils inoculated with R. solani and P. ultimum and Serenade formulation was applied by soil-drenching with 9-, 19-, and 39-fold dilutions. Control values of Serenade formulation on Rhizoctonia damping-off of chili pepper was 58% and 29% for 9- and 19-fold dilutions, respectively. In the case of cucumber Rhizoctonia damping-off, Serenade treatments showed similar control efficacy with damping-off of chili pepper. On the other hand, control efficacy of Serenade formulation on Pythium damping-off of cucumber was less than control effects on Rhizoctonia damping-off. Only Pythium damping-off of chili pepper treated with 9-fold dilution Serenade was statistically different with untreated control. This result suggest that Serenade formulation could be effectively used for controlling Rhizoctonia and Pythium damping-off diseases.

키워드

참고문헌

  1. Agrios, G. N. 2005. Plant diseases caused by fungi. 5th ed. Plant pathology. Elsevier Academic Press, Burlington, USA. 594 pp.
  2. Georgakopoulos, D. G., Fiddaman, P., Leifert, C. and Malathrakis, N. E. 2002. Biological control of cucumber and sugar beet dampingoff caused by Pythium ultimum with bacterial and fungal antagonists. J. Appl. Microbiol. 92: 1078-1086. https://doi.org/10.1046/j.1365-2672.2002.01658.x
  3. Gilardi, G., Manker, D. C., Garibaldi, A. and Gullino, M. L. 2008. Efficacy of the biocontrol agents Bacillus subtilis and Ampelomyces quisqualis applied in combination with fungicides against powdery mildew of zucchini. J. Plant Dis. Prot. 115: 208-213. https://doi.org/10.1007/BF03356265
  4. Handelsman, J. and Stabb, E. V. 1996. Biocontrol of soilborne plant pathogens. Plant Cell 8: 1855-1869. https://doi.org/10.1105/tpc.8.10.1855
  5. Howell, C. R. and Stipanovic, R. D. 1980. Suppression of Pythium ultimum-induced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic, pyoluteorin. Phytopathology 70: 712-715. https://doi.org/10.1094/Phyto-70-712
  6. Islam, M. T. and Faruq, A. N. 2012. Effect of some medicinal plant extracts on damping-off disease of winter vegetable. World Appl. Sci. J. 17: 1498-1503.
  7. Islam, S. Z. and Babadoost, M. 2002. Effect of red-light treatment of seedlings of pepper, pumpkin, and tomato on the occurrence of Phytophthora damping-off. Hort. Sci. 37: 678-681.
  8. Joo, G. J., Kim, J. H. and Kang, S. J. 2002. Isolation and antifungal activity of Bacillus ehimensis YJ-37 as antagonistic against vegetables damping-off fungi. Korean J. Life Sci. 12: 200-207. (In Korean) https://doi.org/10.5352/JLS.2002.12.2.200
  9. Kim, J. H., Choi, Y. H., Kang, S. J., Rhee, I. K. and Joo, G. J. 2002. Biocontrol of vegetables damping-off by Bacillus ehimensis YJ-37. Korean J. Life Sci. 12: 416-422. (In Korean) https://doi.org/10.5352/JLS.2002.12.4.416
  10. Lahlali, G., Peng, B., Gossen, D., McGregor, L., Yu, F. Q., Hynes, R. K., Hwang, S. F., McDonald, M. R. and Boyetchko, S. M. 2012. Evidence that the biofungicide Serenade (Bacillus subtilis) suppresses clubroot on canola via antibiosis and induced host resistance. Phytopathology 103: 245-254.
  11. Mao, W., Lewis, J. A. and Lumsden, R. D. 1997. Seed treatment with a fungal or a bacterial antagonist for reducing corn damping-off caused by species of Pythium and Fusarium. Plant Dis. 81: 450-454. https://doi.org/10.1094/PDIS.1997.81.5.450
  12. Marrone, P. G. 2002. An effective biofungicide with novel modes of action. Pestic. Outl. 13: 193-194. https://doi.org/10.1039/b209431m
  13. Peng, D., Li, S., Chen, C. and Zhou, M. 2014. Combined application of Bacillus subtilis NJ-18 with fungicides for control of sharp eyespot of wheat. Biol. Control 70: 28-34. https://doi.org/10.1016/j.biocontrol.2013.11.013
  14. Stephens, C. T., Herr, L. J. and Schmitthenner, A. F. 1982. Characterization of Rhizoctonia isolates associated with damping-off of bedding plants. Plant Dis. 66: 700-703. https://doi.org/10.1094/PD-66-700
  15. Yang, H. S., Sohn, H. B. and Chung, Y. R. 2002. Biological control of Pythium damping-off of cucumber by Bacillus stearothermophilus YC4194. Res. Plant Dis. 8: 234-238. (In Korean) https://doi.org/10.5423/RPD.2002.8.4.234
  16. Zamanizadeh, H. R., Hatami, N., Aminaee, M. M. and Rakhshandehroo, F. 2011. Application of biofungicides in control of damping disease off in greenhouse crops as a possible substitute to synthetic fungicides. Int. J. Environ. Sci. Tech. 8: 129-136. https://doi.org/10.1007/BF03326202

피인용 문헌

  1. Control of Ginseng Damping-off by Streptomyces sp. A75 and A501 vol.44, pp.4, 2016, https://doi.org/10.4489/KJM.2016.44.4.330