The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Dodam-tang on Cerebral Ischemic Damage of Hyperlipidemic Rats

도담탕(導痰湯)이 고지혈증 흰쥐의 뇌허혈 손상에 미치는 영향

  • Kim, Kyung-Hun (Department of Anatomy-Pointology, Gachon University) ;
  • Kim, Do-Hoon (Oriental Medical Classics & History, Gachon University) ;
  • Kim, Youn-Sub (Department of Anatomy-Pointology, Gachon University)
  • 김경훈 (가천대학교 한의과대학 해부경혈학교실) ;
  • 김도훈 (가천대학교 한의과대학 원전의사학교실) ;
  • 김연섭 (가천대학교 한의과대학 해부경혈학교실)
  • Received : 2014.08.06
  • Accepted : 2014.09.16
  • Published : 2014.09.30
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Abstract

Objectives : Dodam-tang has been reported to have a control effect against the hyperlipidemia and thrombosis. Based upon these previous reports, this study investigates the effects of Dodam-tang on the cerebral ischemic damage of the hyperlipidemic rats. Methods : Hyperlipidemia was induced by the beef tallow 30% diet for 14 days on Sprague-Dawley rats. Ischemic damage was induced by the middle cerebral artery occlusion (MCAO) for 2 hours with the intraluminal thread method. Then water extract of Dodam-tang was administered daily for 5 days. The effect of Dodam-tang was evaluated with the serum lipids, infarct volume and edema percentage, and immunohistochemical expressions of iNOS, MMP-9, and GFAP in the brain tissue. Results : The obtained results were as follows; Dodam-tang reduced significantly the infarct size in a TTC-stained 5th brain section of the hyperlipidemic MCAO rats. Dodam-tang suppressed the infarct volume of the hyperlipidemic MCAO rats, but not significant statistically. Dodam-tang suppressed the edema percentage of the hyperlipidemic MCAO rats significantly in the brain tissue. Dodam-tang suppressed significantly the iNOS expression in the cerebral penumbra and caudate putamen of the hyperlipidemic MCAO rats. Dodam-tang suppressed significantly the MMP-9 expression in the cerebral penumbra of the hyperlipidemic MCAO rats. Dodam-tang suppressed significantly the GFAP-expressed atrocytes in the cerebral penumbra of the hyperlipidemic MCAO rats. Conclusions : These results suggest that Dodam-tang suppresses the brain edema formation through the suppression of the iNOS, MMP-9 and GFAP, but the neuroprotective effect against the cerebral infarct are not distinct.

Keywords

References

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