The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Physiological Activities of Water Extract and Solvent Fractions of an Edible Mushroom, Pholiota adiposa

검은비늘버섯 물 추출물 및 유기용매 분획물의 생리활성 효과

  • Kim, Jun-Ho (Department of Fine Chemistry and New Materials, Sangji University)
  • 김준호 (상지대학교 이공과대학 정밀화학신소재학과)
  • Received : 2014.07.17
  • Accepted : 2014.09.17
  • Published : 2014.09.30
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Abstract

This study was conducted in order to investigate the physiological activities, including antioxidative, fibrinolytic, thrombin inhibitory, and ${\alpha}$-glucosidase inhibitory activities of the water extract and solvent fractions isolated from Pholiota adiposa. The antioxidative activities of the water extract and water fraction were 57.57% and 48.27%, respectively. The fibrinolytic activity was strong only in the ethyl acetate fraction at 0.70 plasmin units/mL. The ethyl acetate fraction showed high thrombin inhibitory activity, and a-glucosidase inhibitory activity at 77.67% and 89.32%, respectively. The ethyl acetate fraction hydrolyzed both $A{\alpha}$ and $B{\beta}$ subunits of human fibrinogen, but did not show reactivity for the ${\gamma}$ form of human fibrinogen. Fibrinolytic activity of the ethyl acetate fraction was not decreased by heating for 10 min at $100^{\circ}C$.

검은비늘버섯을 이용하여 심혈관계 질환과 관련된 기능성 식품 개발을 위한 기초자료를 얻기 위해 검은비늘버섯물 추출물과 유기용매 분획물을 이용하여 심혈관계 질환관련 생리활성을 확인하였다. 물 추출물은 57.57%의 항산화활성을 나타냈지만 혈전용해활성, 트롬빈저해활성, ${\alpha}$-glucosidase 저해효과는 매우 낮게 나타났다. 반면 에틸 아세테이트 분획물은 0.70 plasmin unit의 높은 혈전용해 활성과, 87.36%와 46.44%의 높은 트롬빈 저해활성과 ${\alpha}$-glucosidase 저해활성을 나타냈으며, 물 분획물은 48.27%의 항산화활성을 나타냈다. 혈전용해활성이 높은 에틸 아세테이트 분획물은 섬유소원의 $A{\alpha}$와 $B{\beta}$ chain은 모두 분해시켰지만 ${\gamma}$ chain과는 반응하지 않았으며, $100^{\circ}C$에서 10분간 가열에도 혈전용해 활성에 변화가 없었다. 실험 결과로부터 검은비늘버섯의 에틸아세테이트 분획물은 높은 혈전용해활성과 트롬빈 저해활성과 ${\alpha}$-glucosidase 저해활성을 나타내 심혈관계 질환 관련 치료나 예방을 위한 기능성식품 개발에 이용 가능할 것으로 기대된다.

Keywords

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