Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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MicroRNA-146a Enhances Helicobacter pylori Induced Cell Apoptosis in Human Gastric Cancer Epithelial Cells

  • Wu, Kai (Department of Gastroenterology, 309 Hospital of Chinese People's Liberation Army) ;
  • Yang, Liu (Department of Pathophysiology, Dalian Medical University) ;
  • Li, Cong (Department of Pathophysiology, Dalian Medical University) ;
  • Zhu, Chao-Hui (Department of Pathophysiology, Dalian Medical University) ;
  • Wang, Xin (Department of Gastroenterology, 309 Hospital of Chinese People's Liberation Army) ;
  • Yao, Yi (Department of Gastroenterology, 309 Hospital of Chinese People's Liberation Army) ;
  • Jia, Yu-Jie (Department of Pathophysiology, Dalian Medical University)
  • Published : 2014.07.30
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Abstract

Helicobacter pylori (H. pylori) infection induces apoptosis in gastric epithelial cells, and this occurrence may link to gastric carcinogenesis. However, the regulatory mechanism of H. pylori-induced apoptosis is not clear. MicroRNA-146a has been implicated as a key regulator of the immune system. This report describes our discovery of molecular mechanisms of microRNA-146a regulation of apoptosis in human gastric cancer cells. We found that overexpression of microRNA-146a by transfecting microRNA-146a mimics could significantly enhance apoptosis, and this upregulation was triggered by COX-2 inhibition. Furthermore, we found that microRNA-146a density was positively correlated with apoptosis rates in H. pylori-positive gastric cancer tissues and intratumoral microRNA-146a density was negatively correlated with lymph node metastasis among H. pylori-positive gastric cancer patients. Understanding the important roles of microRNA-146a in regulating cell apoptosis in H. pylori infected human gastric cancer cells will contribute to the development of microRNA targeted therapy in the future.

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