Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Genetic characterization and phylogenetic analysis of Clostridium chauvoei isolated from Hanwoo in Jeonbuk

전북지역 한우에서 분리한 기종저 균의 유전학적 특성 규명

  • Kim, Chul-Min (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research) ;
  • Jeong, Jae-Myong (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research) ;
  • Choi, Ki-Young (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research)
  • 김철민 (전라북도축산위생연구소 정읍지소) ;
  • 정재명 (전라북도축산위생연구소 정읍지소) ;
  • 최기영 (전라북도축산위생연구소 정읍지소)
  • Received : 2014.06.10
  • Accepted : 2014.08.20
  • Published : 2014.09.30
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Abstract

Clostridium chauvoei is the etiologic agent of blackleg, a high mortality rated disease infection mainly cattle. In the present study, the partial sequences of 16S rRNA and flagellin gene of C. chauvoei isolated in Jeonbuk, Korea were determined and compared with those of reference strain. Oligonucleotide primers were designed to amplify a 811 bp fragment of 16S rRNA gene and 1229 bp fragment of flagellin gene. Sequencing analysis of 16S rRNA gene showed high homology to the reference strains ranging 82.3% to 100%, while flagellin gene were different from published foreign clostridia, showing 98.7% to 72.0% nucleotide sequence homology. Phylogenetic analysis based on 16S rRNA gene revealed the close phylogenetic relationship of C. chauvoei and C. septicum in cluster I, which includes C. carnis, C. tertium, C. quinii, C. celatum, C. perfringens, C. absonum, C. botulinum B. Phylogentic analysis also revealed that flagellin gene formed a single cluster with C. chauvoei, C. septicum, C. novyi A, C. novyi B, C. tyrobutylicum, C. acetobutylicum. The genetic informations obtained from this study could be useful for the molecular study of C. chauvoei.

Keywords

References

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