한국식품저장유통학회지 (Food Science and Preservation)

  • 제21권4호
  • /
  • Pages.560-564
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  • 2014
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  • 3022-5477(pISSN)
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  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
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오죽 잎 용매분획물의 항산화 및 항균 활성

Antioxidant and antimicrobial activity of solvent fractions from black bamboo leaves

  • Bae, Hyun-Kyung (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Chung, Shin-Kyo (School of Food Science and Biotechnology, Kyungpook National University)
  • 투고 : 2014.05.26
  • 심사 : 2014.07.15
  • 발행 : 2014.08.30
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초록

오죽 잎의 메탄올 조추출물을 극성에 따라 용매분획하고 분획물의 항산화 활성과 항산화 활성, S. aureus 균주에 대한 항균활성을 조사하고 활성성분을 HPLC를 이용하여 정량하였다. 용매분획물 중에서 ethyl acetate 분획물의 DPPH radical 소거 활성, FRAP활성이 모두 가장 강하였으며, 총페놀성 화합물의 함량도 가장 높았다. S. aureus 균주에 대한 항균활성 역시 ethyl acetate 분획물이 모든 시험 농도에서 가장 높은 활성을 보였다. Ethyl acetate 분획물 중에서 caffeic acid, ferulic acid, quercetin, kaempferol 성분을 분리하여 정량하였으며 이 중 kaempferol의 함량이 가장 높았다. 오죽 잎의 ethyl acetate 분획물은 천연 식품보존료로서 활용이 가능할 것으로 보인다.

To investigate the natural antioxidant and antimicrobial phytochemicals from black bamboo (Phyllostachys nigra MUNRO) leaves, the solvent fractions from crude methanol extract were made with n-hexane, ethyl acetate, and butanol, and their antioxidant and antimicrobial activities were determined. The antioxidant activities were examined by 1,1-diphenyl-1-picrylhydrazyl (DPPH) method and ferric ion reducing antioxidant power (FRAP) method, and the antimicrobial activities against Staphylococcus aureus were tested by paper disc agar diffusion method. Total phenolic contents and total flavonoid contents of the solvent fractions were also determined. The ethyl acetate fraction with the highest total phenolic contents among all fractions showed the strong antioxidant activities by DPPH method and FRAP method, and antimicrobial activities against S. aureus at all test concentrations. Caffeic acid, ferulic acid, quercetin, and kaempferol were analyzed by HPLC in the ethyl acetate fraction from black bamboo leaves by the comparison with the standard chemicals. It is supposed that the ethyl acetate fraction from black bamboo leaves could be used as natural preservatives in the food industry.

키워드

참고문헌

  1. Baek JW, Chung SH, Moon GS (2002) Antimicrobial activities of ethanol extracts from Korean bamboo culms and leaves. Korean J Food Sci Technol, 34, 1073-1078
  2. Kim SM, Jeon JS, Kang SW, Kim WR, Lee KD, Um BH (2012) Composition analysis and antioxidant activity of Ojuk (Phyllostachys nigra Munro) laeaf tea and shoot tea. J Appl Biol Chem, 55, 95-101 https://doi.org/10.3839/jabc.2011.065
  3. Choi SD, Lee KJ (2008) Enhanced extraction and antioxidant activity analysis of flavone c-glycosides from black bamboo using ultrasonic wave. Korean J Biotechnol Bioeng, 23, 297-302
  4. Shang YF, Kim SM, Um BH (2014) Optimisation of pressurised liquid extraction of antioxidants from black bamboo leaves. Food Chem, 154, 164-170 https://doi.org/10.1016/j.foodchem.2013.12.050
  5. Kim YS, Cho KA, Choi DB (2010) Effect of solvents of extraction on the biological activities of Phyllostachys Nigra Munro. Appl Chem Eng, 21, 6-10
  6. Lee HJ, Kim KA, Kang KD, Lee EH, Kim CY, Um BH, Jung SH (2010) The compound isolated from the leaves of Phyllostachys nigra protects oxidative stressinduced retinal ganglion cells death. Food Chem Toxicol, 48, 1721-1727 https://doi.org/10.1016/j.fct.2010.03.052
  7. Blois MS (1958) Antioxidants determination by the use of a stable free radical. Nature, 181, 1199-1200 https://doi.org/10.1038/1811199a0
  8. Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP Assay. Anal Biochem, 239, 70-76 https://doi.org/10.1006/abio.1996.0292
  9. Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenolic and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol, 299, 152-17 https://doi.org/10.1016/S0076-6879(99)99017-1
  10. Jia Z, Tang M, Wu J (1999) The determination of flavonoid contents in mulberry and their scavenging effect on superoxide radicals. Food Chem, 64, 555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  11. Park SW, Woo CJ, Chung SK, Chung KT (1994) Antimicrobial and antioxidative activities of solvent fraction from Humulus japonicus. Korean J Food Sci Technol, 26, 464-470
  12. Kang SK, Kim YD, Choi OJ (1998) Antimicrobial activity of defatted camellia (Camellia japonica L.) seed extract. J Korean Soc Food Sci Nutr, 27, 232-238
  13. Jang MW, Ha BJ (2012) Effects of broccoli on anti-inflammation and anti-oxidation according to extraction solvent. J Fd Hyg Safety, 27, 461-465 https://doi.org/10.13103/JFHS.2012.27.4.461
  14. Kang SJ, Chung SK (2012) Antioxidant and antimicrobial activities of Korean mistletoe (Viscum album var. coloratum) extracts against food poisoning bacteria. Korean J Food Preserv, 19, 919-924 https://doi.org/10.11002/kjfp.2012.19.6.919
  15. Lee JH, Lee SR (1994) Some physiological activity of phenolic substances in plant foods. Korean J Food Sci Technol, 26, 317-323
  16. Folin O, Denis W (1912) On phosphotungastic phosphomolybdic compounds as color reagents. J Biol Chem, 12, 239-249.
  17. Proteggente AR, Pannala AS, Paganga G, Van BL, Wagner E, Wiseman S, Van DPF, Dacombc C, Rice- Evans CA (2002) The antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin composition. Free Radical Res, 36, 217-233 https://doi.org/10.1080/10715760290006484
  18. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS (2005) Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J Food Sci Technol, 37, 233-240
  19. Moon JS, Kim SJ, Park YM, Hwang IS, Kim EH, Park JW, Park IB, Kim SW, Kang SG, Park YK, Jung ST (2004) Antimicrobial effect of methanol extracts from some medicinal herbs and the content of phenolic compounds. Korean J Food Preserv, 11, 207-213
  20. Clark AM, El-Feraly FS, Li WW (1981) Antimicrobial activity of phenolic constituents of Mangnolia grandiflora L. J Pharm Sci, 70, 951-952 https://doi.org/10.1002/jps.2600700833
  21. Kim JY, Lee JA, Yoon WJ, Oh DJ, Jung YH, Lee WJ, Park SY (2006) Antioxidative and antimicrobial activities of Euphorbis jolkini extracts. Korean J Food Sci Technol, 38, 699-706