Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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The anti-climacterium effects of red clover dry extracts combined with pomegranate concentration powder in ovariectomized rats

  • Kim, Kyung Hu (Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University) ;
  • Kang, Su Jin (The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University) ;
  • Choi, Beom Rak (Research Institute, Health-Love Co., Ltd.) ;
  • Kim, Seung Hee (Research Institute, Health-Love Co., Ltd.) ;
  • Yi, Hae Yeon (Research Institute, Health-Love Co., Ltd.) ;
  • Kim, Dong Chul (Department of Korean Obstetrics & Gynecology, College of Korean Medicine, Daegu Haany University) ;
  • Choi, Seong Hun (Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University) ;
  • Han, Chang Hyun (Department of Medical History & Literature Group, Korea Institute of Oriental Medicine) ;
  • Park, Soo Jin (Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University) ;
  • Song, Chang Hyun (Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University) ;
  • Ku, Sae Kwang (Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Young Joon (Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University)
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  • 구세광 (대구한의대학교 한의과대학 해부학교실) ;
  • 이영준 (대구한의대학교 한의과대학 해부학교실)
  • Received : 2014.07.21
  • Accepted : 2014.08.21
  • Published : 2014.08.31
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Abstract

Objective : In this study, the addition of dried pomegranate concentrate powder (PCP) was affected the anti-climacterium activity of red clover dry extracts (RC) in ovariectomized (OVX) rats. Materials and methods : After bilateral OVX surgery, RC 40 mg/kg, PCP 20 mg/kg and RC:PCP 2:1 mixture (g/g) 120, 60 and 30 mg/kg (of body weight) were orally administered, once a day for 84 days, and then the changes on the serum estradiol levels, abdominal fat pad and uterus weights were observed for estrogenic effects. In addition, liver weights, serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were also evaluated for hepatoprotective effects, and serum total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL) and triglyceride (TG) levels were monitored for hypolipidemic effects. Results : As a result of OVX, the estrogen-deficient climacterium symptoms, increments of abdominal fat pad weights, serum AST, ALT, TC, LDL and TG levels with decrease of uterus and liver weights, serum estradiol levels, were demonstrated. However, these estrogen-deficient climacterium symptoms induced by bilateral OVX in rats were significantly inhibited by continuous oral treatment of RC 40 mg/kg, PCP 20 mg/kg and RC:PCP 2:1 mixture (g/g) 120, 60 and 30 mg/kg, respectively. Conclusion : The results suggested that RC:PCP 2:1 mixtures synergistically increased the anti-climacterium effects of RC in OVX rats. It, therefore, is expected that RC:PCP 2:1 mixture will be promising as a new potent protective agents for relieving the climacterium symptoms.

Keywords

References

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