Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effects of Phytoplant Diets on Body Weight, Feces Production, Body Fat, and Serum Lipid Levels in High-fat Diet-induced Hyperlipidemic Rats

식물성 식이조성물이 고지혈증을 유발시킨 흰쥐의 체중, 배변량, 체지방 및 혈청 지질농도에 미치는 영향

  • Received : 2014.03.14
  • Accepted : 2014.06.09
  • Published : 2014.08.31
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Abstract

This study was performed to evaluate the anti-obesity and lipid-lowering effects of phytoplant diets in rats fed with a high-fat/cholesterol diet (HFCD). Experimental diet formulae contained various phytoplants such as brown rice, barley, soybean, germinated brown rice, malt, black bean, sea tangle, and/or dietary fibers including polydextrose, garcinia combogia, glucomannan, ${\small}L$-carnitine, and chitosan. Male Sprague-Dawley rats were fed with a HFCD for 6 weeks and then fed with a HFCD with/without phytoplants for another 6 weeks. Rats fed with phytoplant diets showed lower body weights, liver weights, visceral fat levels, and blood lipid levels compared to those of rats fed with HFCD alone. In addition, rats administered phytoplant diets showed increased daily feces production during the second experimental phase. These results suggest that phytoplant diets improve body weight, feces production, adipose tissue weight, and lipid metabolism.

본 연구에서는 흰쥐에게 고지방-고콜레스테롤 식이를 6주간 급여하여 고지혈증을 유발한 후, 현미, 발아현미, 보리, 맥아, 대두, 흑두 등이 혼합된 식물성 식이조성물을 각각 다른 비율로 6주간 급여하면서 체중감량, 배변량, 체지방 및 혈청지질농도에 미치는 영향을 조사하였다. 실험기간 동안 일당증체량은 식물성 식이조성물군이 HFCD 대조군과 비교할 때 유의적으로 낮았으며, 배변량은 증가하고 장 통과시간은 HFCD 대조군에 비하여 감소하였다. 내장지방 중 신장주변지방패드(RFP)와 정소상체지방패드(EPF)는 일반식이 대조군에 비하여 고지방-고콜레스테롤 식이를 급여한 HFCD 대조군에서 증가하였으며, 식물성 식이조성물군에서는 HFCD대조군에 비하여 통계적으로 유의하게 감소하였다. 고지방-고콜레스테롤 첨가로 인하여 HFCD 대조군에서 간 무게가 증가하였고, 반면, 식물성 식이조성물군은 HFCD 대조군에 비하여 간조직의 무게가 유의하게 감소하는 경향을 보였다. 또한 고지방-고콜레스테롤 식이에 의해 증가된 혈청 지질 중 TC, TG, LDL-콜레스테롤 농도가 식물성 식이조성물군에서 유의하게 감소하였으며, 동맥경화지수 역시 감소하였다. 이상에서 고지방-고콜레스테롤 식이를 급여한 흰쥐에게 발아현미, 흑두, 검정깨, 다시마, 녹차 등의 식이조성물 첨가식이를 급여했을 때 체중감소, 배변량 증가, 체지방함량 저하, 혈청 중 TC, TG 감소의 유의한 감소작용이 있는 것으로 나타나서, 앞으로 이들 혼합조성물의 적절한 처방으로 다이어트와 고지혈증 개선을 위한 소재로 이용될 수 있을 것으로 사료된다. 또한, 본 연구는 고지혈증 개선 효능이 있는 생식제품 개발시 원료의 레시피 선정에 있어서 기초자료로 활용 가능한 것으로 판단된다.

Keywords

References

  1. Jang YS, Jeong JM. Effects of phyto-extract mixture on adiposity and serum lipid levels in obese mice induced by high fat diet. J. Korean Soc. Food Sci. Nutr. 39: 1439-1445 (2010) https://doi.org/10.3746/jkfn.2010.39.10.1439
  2. Lee HK. Korean disease pattern and nutrition. Korean J. Nutr. 29: 381-383 (1996)
  3. Manninen V, Tenkanen L, Koskinen P, Huttunen JK, Manttari M, Heinonen OP, Frick MH. Joint effects of serum triglyceride and LDL cholesterol and HDL cholesterol concentrations on coronary heart disease risk in the Helsinki Heart Study. Implications for treatment. Circulation 85: 37-45 (1992) https://doi.org/10.1161/01.CIR.85.1.37
  4. Rana JS, Boekholdt SM, Kastelein JJ, Shah PK. The role of non-HDL cholesterol in risk stratification for coronary artery disease. Curr. Atheroscler. Rep. 14: 130-134 (2012) https://doi.org/10.1007/s11883-011-0224-x
  5. McKenney JM. Lipid management: tools for getting to the goal. Am. J. Manag. Care 7: S299-306 (2001)
  6. Miettinen TA. Cholesterol absorption inhibition: a strategy for cholesterol-lowering therapy. Int. J. Clin. Pract. 55: 710-716 (2001)
  7. Crowe FL, Appleby PN, Travis RC, Key TJ. Risk of hospitalization or death from ischemic heart disease among British vegetarians and nonvegetarians: results from the EPIC-Oxford cohort study. Am. J. Clin. Nutr. 97: 597-603 (2013) https://doi.org/10.3945/ajcn.112.044073
  8. Burr ML, Butland BK. Heart disease in British vegetarians. Am. J. Clin. Nutr. 48: 830-832 (1988)
  9. Mason EE. Methods for voluntary weight loss and control. Obes. Surg. 2: 275-276 (1992) https://doi.org/10.1381/096089292765560187
  10. Kim SG, An GH, Yoon SW, Lee YC, Ha SD. A study on dietary supplement to reduce obesity by the mechanism of decreasing lipid and carbohydrate absorption. Korean J. Food Sci. Technol. 35: 519-526 (2003)
  11. Lee JJ, Park MR, Kim AR, Lee MY. Effects of ramie leaves on improvement of lipid metabolism and anti-obesity effect in rats fed a high fat/high cholesterol diet. Korean J. Food Sci. Technol. 43: 83-90 (2011) https://doi.org/10.9721/KJFST.2011.43.1.083
  12. Rudel LL, Morris MD. Determination of cholesterol using O-phthalaldehyde. J. Lipid Res. 14: 364-366 (1973)
  13. Noma A, Nakayama KN, Kita M, Okabe H. Stimultaneous determination of serum cholesterol in high and low density lipoprotein with use of heparin, $Ca^{2+}$, and an anion-exchange resin. Clin. Chem. 24: 1504-1508 (1978)
  14. Park SH, Lee YK, Lee HS. The effects of dietary fiber feeding on gastrointestinal functions and lipid and glucose metabolism in streptozotocin-induced diabetic rats. Kor. J. Nutr. 27: 311-322 (1994)
  15. Kim HK, Cho DW, Hahm YT. The effects of coix bran on lipid metabolism and glucose challenge in hyperlipidemic and diabetic rats. J. Korean Soc. Food Sci. Nutr. 29: 140-146 (2000)
  16. Han CK, Kim SS, Choi SY, Park JH, Lee BH. Effects of rice added with mulberry leaves and fruit on blood glucose, body fat and serum lipid levels in rats. J. Korean Soc. Food Sci. Nutr. 38: 1336-1341 (2009) https://doi.org/10.3746/jkfn.2009.38.10.1336
  17. Chai YM, Lim Bk, Lee JY, Kim YH, Rhee SJ. Preparation of soluble dietary fiber from oak wood (Quereus Mongolica) and its physiological function in rat fed high cholesterol diets. J. Korean Nutr. Soc. 36: 9-17 (2003)
  18. Spiller GA, Chernoff MC, Hill RA, Gates JE, Nassar JJ, Shipley EA. Effect of purified cellulose, pectin, and a low-residue diet on fecal volatile fatty acids, transit time, and fecal weight in humans. Am. J. Clin. Nutr. 33: 754-759 (1980)
  19. Kang HJ, Song YS. Dietary fiber and cholesterol metabolism. J. Korean Soc. Food Sci. Nutr. 26: 358-369 (1997)
  20. Lee KW, Sung KS, Kim SS, Lee OH, Lee BH, Han CK. Effects of Cucurbita moschata, adlay seed, and Cudrania tricuspidata leaf mixed-powder diet supplements on the visceral fat, fecal amount, and serum lipid levels of the rats on a high-fat diet. Korean J. Food Nutr. 25: 990-998 (2012) https://doi.org/10.9799/ksfan.2012.25.4.990
  21. Jung UJ, Lee JS, Bok SH, Choi MS. Effects of extracts of persimmon leaf, buckwheat leaf, and Chinese matrimony vine leaf on body fat and lipid metabolism in rats. J. Korean Soc. Food Sci. Nutr. 40: 1215-1226 (2011) https://doi.org/10.3746/jkfn.2011.40.9.1215
  22. Despres JP. Abdominal obesity as important component of insulin-resistant syndrome. Nutrition 9: 452-459 (1993)
  23. Story JA. The role of dietary fiber in lipid metabolism. Adv. Lipid Res. 18: 229-246 (1981) https://doi.org/10.1016/B978-0-12-024918-3.50012-5
  24. Jayasooriya AP, Sakono M, Yukizaki C, Kawano M, Yamamoto K, Fukuda N. Effects of Momordica charantia powder on serum glucose levels and various lipid parameters in rats fed with cholesterol-free and cholesterol-enriched diets. J. Ethnopharmacol. 72: 331-336 (2000) https://doi.org/10.1016/S0378-8741(00)00259-2
  25. Golan M, Fainaru M, Weizman A. Role of behaviour modification in the treatment of childhood obesity with the parents as the exclusive agents of change. Int. J. Obes. Relat. Metab. Disord. 22: 1217-1224 (1998) https://doi.org/10.1038/sj.ijo.0800749
  26. Lee JM, Lee SH, Lee JH, Lee E. Effects of Coix lachrymal-jobi var. mayuen Stapf. pharmacopuncture on plasma lipid composition and glucose in rat fed high fat diet. Kor. J. Acupunct. 23:59-66 (2006)
  27. Jang JY, Choi HY. Effects of Artemisia iwayomogi oligosaccharide on the blood lipids, abdominal adipose tissues and leptin levels in the obese rats. Korean J. Nutr. 36: 437-445 (2003)
  28. Joo DS, Lee JK, Choi YS, Cho SY, Je YK, Choi JW. Effect of seatangle oligosaccharide drink on serum and hepatic lipids in rats fed a hyperlipidemic diet. J. Korean Soc. Food Sci. Nutr. 32: 1364-1369 (2003) https://doi.org/10.3746/jkfn.2003.32.8.1364
  29. Jung YH, Han SH, Shin MK. Effects of green and black Korean teas on lipid metabolism in diet-induced hyperlipidemic rats. J. East Asian Soc. Dietary Life 16: 550-558 (2006)
  30. Lee E, Choi MY. Effects of pine needle on lipid composition and TBARS in rat fed high cholesterol. Korean. J. Food Sci. Technol. 32: 1186-1190 (2000)
  31. Sin MK, Han SH. Effects of methanol extracts from Phaseolus vulgaris on serum lipid concentration in rats fed high fat and cholesterol diet. Korean. J. Food Sci. Technol. 33: 113-116 (2001)
  32. Jung KJ, Jung BM, Kim SB. Effects of porphyran isolated from laver, Porphyra yezoensis, on lipid metabolism in hyperlipidemic and hypercholesterolemic rats. Korean. J. Food Sci. Technol. 33: 633-640 (2001)
  33. Potter SM, Baum JA, Teng H, Stillman RJ, Shay NF, Erdman JW Jr. Soy protein and isoflavones: their effects on blood lipids and bone density in postmenopausal women. Am. J. Clin. Nutr. 68: 1375S-1379S (1998)
  34. Clair RS, Anthony M. Soy, isoflavones and atherosclerosis. Handb. Exp. Pharmacol. 170: 301-323 (2005) https://doi.org/10.1007/3-540-27661-0_10
  35. Potter SM. Overview of proposed mechanisms for the hypocholesterolemic effect soy. J. Nutr. 125: 606S-611S (1995)
  36. Story JA, Kritchevsky D. Comparison of the binding of various bile acids and bile salts in vitro by several types of fiber. J. Nutr. 106: 1292-1294 (1976)
  37. Anderson JW, Chen WJ. Plant fiber. Carbohydrate and lipid metabolism. Am. J. Clin. Nutr. 32: 346-363 (1979)
  38. Venter CS, Vorster HH, Van der Nest DG. Comparison between physiological effects of konjac-glucomannan and propionate in baboons fed "Western" diets. J. Nutr. 120: 1046-1053 (1990)

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