Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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An Enantioselective Amidase from Burkholderia multivorans for the Stereoselective Synthesis of Esfenvalerate

  • Lee, Sang-Hyun (Department of Biotechnology, Korea University Graduate School) ;
  • Park, Oh-Jin (Department of Biological and Chemical Engineering, Yanbian University of Science and Technology) ;
  • Shin, Hyun-Jae (Department of Chemical and Biochemical Engineering, Chosun University)
  • Received : 2014.01.10
  • Accepted : 2014.04.11
  • Published : 2014.07.28
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Abstract

Using racemic (R,S)-2-(4-chlorophenyl)-3-methylbutyramide, an intermediate for the chiral pyrethroid insecticide Esfenvalerate, as a sole nitrogen source in a minimal medium, several strains with high enatioselectivity (${\geq}98%$) were isolated by enrichment techniques. One of the strains, LG 31-3, was identified as Burkholderia multivorans, based on physiological and morphological tests by a standardized Biolog station for carbon source utilization. A novel amidase was purified from B. mutivorans LG 31-3 and characterized. The enzyme exhibited (S)-selective amidase activity on racemic (R,S)-2-(4-chlorophenyl)-3-methylbutyramide. Addition of the racemic amide induced the production of the enantioselective amidase. The molecular mass of the amidase on SDS-PAGE analysis was shown to be 50 kDa. The purified amidase was subjected to proteolytic digestion with a modified trypsin. The N-terminal and internal amino acid sequences of the purified amidase showed a high sequence homology with those deduced from a gene named YP_366732.1 encoding indole acetimide hydrolase from Burkholderia sp. 383.

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References

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