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A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus

  • Bang, Bongjun (Department of Microbiology, Pusan National University) ;
  • Lee, Jongyun (Department of Microbiology, Pusan National University) ;
  • Kim, Sunyoung (Department of Microbiology, Pusan National University) ;
  • Park, Jungwook (Department of Microbiology, Pusan National University) ;
  • Nguyen, Thao Thi (Department of Microbiology, Pusan National University) ;
  • Seo, Young-Su (Department of Microbiology, Pusan National University)
  • 투고 : 2014.03.26
  • 심사 : 2014.05.08
  • 발행 : 2014.09.01

초록

Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, is responsible for one of the most devastating viral diseases in tomato-growing countries and is becoming a serious problem in many subtropical and tropical countries. The climate in Korea is getting warmer and developing subtropical features in response to global warming. These changes are being accompanied by TYLCV, which is now becoming a large problem in the Korean tomato industry. The most effective way to reduce damage caused by TYLCV is to breed resistant varieties of tomatoes. To accomplish this, it is necessary to establish a simple inoculation technique for the efficient evaluation of resistance to TYLCV. Here, we present the rolling circle amplification (RCA) method, which employs a bacteriophage using phi-29 DNA polymerase for construction of infectious TYLCV clones. The RCA method is simple, does not require sequence information for cloning, and is less expensive and time consuming than conventional PCR based-methods. Furthermore, RCA-based construction of an infectious clone can be very useful to other emerging and unknown geminiviruses in Korea.

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참고문헌

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피인용 문헌

  1. Comparative Analyses of Tomato yellow leaf curl virus C4 Protein-Interacting Host Proteins in Healthy and Infected Tomato Tissues vol.32, pp.5, 2016, https://doi.org/10.5423/PPJ.FT.08.2016.0165
  2. CRISPR/Cas9-mediated viral interference in plants vol.16, pp.1, 2015, https://doi.org/10.1186/s13059-015-0799-6
  3. Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives vol.8, 2017, https://doi.org/10.3389/fpls.2017.00360
  4. Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification vol.10, pp.9, 2018, https://doi.org/10.3390/v10090469