The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Rpi-blb2-Mediated Hypersensitive Cell Death Caused by Phytophthora infestans AVRblb2 Requires SGT1, but not EDS1, NDR1, Salicylic Acid-, Jasmonic Acid-, or Ethylene-Mediated Signaling

  • Oh, Sang-Keun (Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University) ;
  • Kwon, Suk-Yoon (Korea Research Institute of Bioscience & Biotechnology) ;
  • Choi, Doil (Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University)
  • 투고 : 2014.03.31
  • 심사 : 2014.04.07
  • 발행 : 2014.09.01
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초록

Potato Rpi-blb2 encodes a protein with a coiled-coil-nucleotide binding site and leucine-rich repeat (CC-NBSLRR) motif that recognizes the Phytophthora infestans AVRblb2 effector and triggers hypersensitive cell death (HCD). To better understand the components required for Rpi-blb2-mediated HCD in plants, we used virus-induced gene silencing to repress candidate genes in Rpi-blb2-transgenic Nicotiana benthamiana plants and assayed the plants for AVRblb2 effector. Rpi-blb2 triggers HCD through NbSGT1-mediated pathways, but not NbEDS1- or NbNDR1-mediated pathways. In addition, the role of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) in Rpi-blb2-mediated HCD were analyzed by monitoring of the responses of NbICS1-, NbCOI1-, or NbEIN2-silenced or Rpi-blb2::NahG-transgenic plants. Rpi-blb2-mediated HCD in response to AVRblb2 was not associated with SA accumulation. Thus, SA affects Rpi-blb2-mediated resistance against P. infestans, but not Rpi-blb2-mediated HCD in response to AVRblb2. Additionally, JA and ET signaling were not required for Rpi-blb2-mediated HCD in N. benthamiana. Taken together, these findings suggest that NbSGT1 is a unique positive regulator of Rpi-blb2-mediated HCD in response to AVRblb2, but EDS1, NDR1, SA, JA, and ET are not required.

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참고문헌

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피인용 문헌

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  2. Virus-induced gene silencing reveals signal transduction components required for the Pvr9 -mediated hypersensitive response in Nicotiana benthamiana vol.495, 2016, https://doi.org/10.1016/j.virol.2016.05.011
  3. SGT1 is required in PcINF1/SRC2-1 induced pepper defense response by interacting with SRC2-1 vol.6, pp.1, 2016, https://doi.org/10.1038/srep21651
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