과제정보
연구 과제 주관 기관 : Ministry of Science, ICT and Future Planning (MSIP)
방사선산업학회지 (Journal of Radiation Industry)
- 제8권2호
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- Pages.65-69
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- 2014
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- 1976-2402(pISSN)
Cloning of Cellobiohydrolase Gene (cbhI) in Radiation Induced Mutant of Pleurotus florida
- Lee, Young-Keun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
- Sathesh-Prabu, Chandran (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
- Kim, Min Kyung (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
- 투고 : 2014.06.16
- 심사 : 2014.08.24
- 발행 : 2014.09.30
초록
The cellobiohydrolase gene (cbhI), a key component of a cellulolytic system, of a mutant PfCM4 (Pleurotus florida), developed through gamma ray radiation mutagenesis, was isolated and cloned. The deduced amino acid sequence was closely related to the glycoside hydrolase family 7 (GH7). The molecular weight of the deduced amino acid sequence of cbhI gene was found to be 22.4 kDa. Though the percent identity was found to be much less (35.61%) between the wild type and mutant, the cellulolytic activity of PfCM4 was 17.24% higher than that of the wild type. This shows that the catalytic domain of the cbhI gene was conserved in the mutant PfCM4.