Quantitative Analysis of the Marker Components in Glycyrrhizae Radix et Rhizoma by Processing Method

포제(炮製)에 따른 감초 중 liquiritin, glycyrrhizin 및 glycyrrhetinic acid의 함량분석

  • Seo, Chang-Seob (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Jung-Hoon (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine) ;
  • Hwang, Seock-Yeon (Department of Biomedical Laboratory Science, College of Natural Science, Daejeon University) ;
  • Kim, Byoung-Soo (Department of Physiology, College of Korean Medicine, Daejeon University)
  • Received : 2014.07.01
  • Accepted : 2014.07.31
  • Published : 2014.08.20

Abstract

Glycyrrhizae Radix et Rhizoma has been extensively used by human beings as a medicinal herb as well as natural sweetener. In this study, we performed quantification analysis of three major constituents including liquiritin, glycyrrhizin, and glycyrrhetinic acid in the 70% ethanol extracts of non-processed Glycyrrhizae Radix et Rhizoma and processed Glycyrrhizae Radix et Rhizoma using a high-performance liquid chromatography coupled with photodiode array detector. The analytical column for separation of the 3 constituents used a Gemini C18 column kept at $40^{\circ}C$ by the gradient elution of two mobile phase. The amounts of liquiritin, glycyrrhizin, and glycyrrhetinic acid in non-processed Glycyrrhizae Radix et Rhizoma were 2.57%, 3.52%, and not detected. After processing by roasting, the best roasting temperature and time of iquiritin, glycyrrhizin, and glycyrrhetinic acid were $160^{\circ}C$-15 min (2.46%), $160^{\circ}C$-15 min (3.67%), and $240^{\circ}C$-15 min (0.76%), respectively.

Keywords

References

  1. 생약학교재편찬위원회. 생약학. 서울, 동명사, pp.104-107. 2012.
  2. 한방약리학교재편찬위원회. 한방약리학. 서울:신일북스. 2010: 116-120.
  3. Han SB, Gu HA, Kim SJ, Kim HJ, Kwon SS, Kim HS, Jeon SH, Hwang JP, Park SN. Comparative study on antioxidative activity of Glycyrrhiza uralensis and Glycyrrhizia glabra extracts by country of origin. J Soc Cosmet Scientists Korea, 39(1):1-8, 2013.
  4. Kim SJ, Kweon DH, Lee JH. Investigation of antioxidative activity and stability of ethanol extract of licorice roots (Glycyrrhiza glabra). Korean J Food Sci Technol., 38(4), 584-588, 2006.
  5. Yoon TS, Cheon MS, Kim SJ, Lee AY, Moon BC, Chun JM, Choo BK, Kim HK. Evaluation of solvent extraction on the anti-inflammatory efficacy. Korean J Medicinal Crop Sci., 18(1), 28-33, 2010.
  6. Wang W, Luo M, Fu Y, Wang S, Efferth T, Zu Y. Glycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid. Int J Nanomedicine, 8(1), 1377-1383, 2013.
  7. Yang XL, Liu D, Bian K, Zhand DD. Study on in vitro anti-inflammatory activity of total flavonoids from Glycyrrhizae Radix et Rhizoma and its ingredients. Zhongguo Zhong Yao Za Zhi, 38(1), 99-104, 2013.
  8. Ahn EY, Shin DH, Back NI, Oh JA. Isolation and identification of antimicrobial active substance from Glycyrrhiza uralensis. Korean J Food Sci Technol., 30(3), 680-687, 1998.
  9. Park JH, Wu Q, Yoo KH, Yong HI, Cho SM, Chung IS, Back NI. Cytotoxic effect of flavonoids from the roots of Glycyrrhiza uralensis on human cancer cell lines. J Appl Biol Chem., 54(1), 67-70, 2011. https://doi.org/10.3839/jabc.2011.012
  10. Feng Yeh C, Wang KC, Chiang LC, Shieh DE, Yen MH, San Chang J. Water extract of licorice had anti-viral activity against human respiratory syncytial virus in human respiratory tract cell lines. J Ethnopharmacol., 148(2), 466-473, 2013. https://doi.org/10.1016/j.jep.2013.04.040
  11. Adianti M, Aoki C, Komoto M, Deng L, Shoji I, Wahyuni TS, Lusida MI, Soetjipto, Fuchino H, Kawahara N, Hotta H. Anti-hepatitis C virus compounds obtained from Glycyrrhiza uralensis and other Glycyrrhiza species. Microbiol Immunol., 58(3), 180-187, 2014. https://doi.org/10.1111/1348-0421.12127
  12. 동의학연구소. 한약제법. 서울, 여강출판사, p.62. 1994.
  13. Lee JR, Jo MJ, Park SM, Kim SC, Park SJ. Establishment of UPLC method for analysis of liquiritingenin and studies on the processing of licorice for enhancement of liquiritigenin content. Korean J Oriental Med Prescrip., 18(1), 145-154, 201.
  14. Kim JS, Kim HJ, Ma JY, Kim JM. Studies on the processing of herbal medicines (II) - HPLC analysis of standard compounds of unprocessedand processed herbal medicines - Kor J Pharmacogn., 33(4), 305-307, 2002.
  15. 강병수, 서부일, 최호영. 한약 포제와 임상응용. 서울, 영림사, pp.46-69. 2003.
  16. Woo KS, Jang KI, Kim KY, Lee JB, Jeong JS. Antioxidative activity of heat treated licorice (Glycyrrhiza uralensis Fisch) extracts. Korean J Food Sci Techonl., 38(3), 355-360, 2006.
  17. Hwang IK, Lim SS, Choi KH, Yoo KY, Shin HK, Kim EJ, Yoon-Park JH, Kang TC, Kim YS, Kwon DY, Kim DW, Moon WK, Won MH. Neuroprotective effects of roasted licorice, not raw form, on neuronal injury in gerbil hippocampus after transent forebrain ischemia. Acta Pharmacol Sin., 27(8), 959-965, 2006. https://doi.org/10.1111/j.1745-7254.2006.00346.x
  18. 전국한의과대학 공동교재편찬위원회 편저. 본초학. 서울, 영림사, p.587. 2004.
  19. 식품의약품안전청. 대한약전외한약(생약)규격집. 식약청고시 제2011-26호. 2011.
  20. 한국약학대학협의회 약전분과회. 제9개정 대한약전해설서 II. 서울, 신일북스, p.1099. 2008.
  21. 한국약학대학협의회 약전분과회. 제9개정대한약전해설서 I. 서울, 신일북스, pp.103-105. 2008.
  22. 식품의약품안전청. 식품의약품안전청 고시 제2011-42호. 2011.