Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Safety Effects against Nitrite and Nitrosamine as well as Anti-mutagenic Potentials of Kale and Angelica keiskei Vegetable Juices

케일과 신선초 채소즙의 안전성 및 항돌연변이 효과

  • Kim, Jong-Dai (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Jong Seok (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Jung, Hye-Youn (Department of Food Science and Nutrition, Pusan National University) ;
  • Kim, Bohkyung (Department of Food Science and Nutrition, Pusan National University) ;
  • Park, Kun-Young (Department of Food Science and Nutrition, Pusan National University)
  • 김종대 (강원대학교 식품생명공학과) ;
  • 이옥환 (강원대학교 식품생명공학과) ;
  • 이종석 (강원대학교 식품생명공학과) ;
  • 정혜연 (부산대학교 식품영양학과) ;
  • 김보경 (부산대학교 식품영양학과) ;
  • 박건영 (부산대학교 식품영양학과)
  • Received : 2014.03.24
  • Accepted : 2014.06.06
  • Published : 2014.08.31
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Abstract

Vegetables contain high levels of nitrate, which can be converted to nitrite for reaction with secondary amines to form nitrosamines. In this study, we evaluated safety effects against nitrite and nitrosamine as well as anti-mutagenic activities of vegetable juices. To do this, the contents of nitrate, nitrite, and nitrosamines were determined in vegetable juices. The safety effects against nitrite and nitrosamine formation were also investigated under simulated human gastric conditions. The contents of nitrate and nitrite in common and organic kale and Angelica keiskei juices were 931~2,052 mg/kg and 13~82 mg/kg, respectively. However, seven kinds of nitrosamines were not detected in the vegetables juices. The nitrate content decreased when vegetable juices were digested under simulated human gastric conditions. Nitrosamine (N-nitrosodimethylamine) formation under simulated human gastric conditions was inhibited by addition of vegetable juices. In addition, vegetable juices, especially organically cultivated juices, showed anti-mutagenic effects in a Salmonella assay system. These results suggest that organically cultivated vegetable juices are a promising health-promoting source.

본 연구에서는 유기농 및 일반농 케일과 신선초를 착즙하여 다양한 실험을 통하여 안전성 및 항돌연변이 활성을 비교하였다. 유기농 채소즙의 안전성 측정을 위해 질산염과 아질산염 함량을 분석한 결과 케일즙의 경우 유기농 및 일반농의 함량이 유사하게 나타났다. 신선초즙의 경우 유기농이 일반농보다 질산염 및 아질산염 함량 모두 유의적으로 낮게 나타났다. 한편 7종류의 니트로사민 모두가 이들 채소즙에서 검출되지 않았다. 니트로사민의 저해물들이 반응하는 위에서의 변화를 알아보기 위하여 인공위액에서의 소화과정 중 질산염과 아질산염 함량을 분석한 결과, 유기농 및 일반농 채소즙의 질산염 함량은 인공소화 전보다 후의 함량이 현저히 낮아졌다. 인공 위액계에서 채소즙은 아질산염과 2급 아민에 의해 생성되는 니트로사민의 생성을 오히려 크게 억제하는 효과가 있었다. 질산염과 아질산염을 함유하고 있는 채소즙의 돌연변이 유발 및 저해 효과를 살펴보기 위해 Ames test를 한 결과 항돌연변이 활성이 있었으며, 유기농 채소즙의 항돌연변이 효과가 일반농에 비해 우수한 것으로 나타났다. 이상의 실험 결과 채소즙의 주재료가 되는 유기농 케일 및 신선초는 질산염, 아질산염 등의 함량이 낮을 뿐만 아니라 소화과정에서 전환되어 니트로사민을 생성하지 않았고 항돌연변이 효과도 우수한 것으로 나타났다.

Keywords

References

  1. Halliwell B, Murcia MA, Chirico S, Aruoma OI. 1995. Free radicals and antioxidants in food and in vivo: what they do and how they work. Crit Rev Food Sci Nutr 35: 7-20. https://doi.org/10.1080/10408399509527682
  2. Bhardwaj RL, Pandey S. 2011. Juice blends－a way of utilization of under-utilized fruits, vegetables, and spices: A review. Crit Rev Food Sci Nutr 51: 563-570. https://doi.org/10.1080/10408391003710654
  3. Scalbert A, Williamson G. 2000. Dietary intake and bioavailability of polyphenols. J Nutr 130: 2073S-2085S.
  4. Riccioni G, D'Orazio N, Salvatore C, Franceschelli S, Pesce M, Speranza L. 2012. Carotenoids and vitamins C and E in the prevention of cardiovascular disease. Int J Vitam Nutr Res 82: 15-26. https://doi.org/10.1024/0300-9831/a000090
  5. Zhang J, Hou X, Ahmad H, Zhang H, Zhang L, Wang T. 2014. Assessment of free radicals scavenging activity of seven natural pigments and protective effects in AAPHchallenged chicken erythrocytes. Food Chem 145: 57-65. https://doi.org/10.1016/j.foodchem.2013.08.025
  6. Arroqui C, Rumsey TR, Lopez A, Virseda P. 2001. Effect of different soluble solids in the water on the ascorbic acid losses during water blanching of potato tissue. J Food Eng 47: 123-126. https://doi.org/10.1016/S0260-8774(00)00107-2
  7. Howard LA, Wong AD, Perry AK, Klein BP. 1999. ${\beta}$-Carotene and ascorbic acid retention in fresh and processed vegetables. J Food Sci 64: 929-936. https://doi.org/10.1111/j.1365-2621.1999.tb15943.x
  8. White JW Jr. 1975. Relative significance of dietary sources of nitrate and nitrite. J Agric Food Chem 23: 886-891. https://doi.org/10.1021/jf60201a034
  9. Gangolli SD, van den Brandt PA, Feron VJ, Janzowsky C, Koeman JH, Speijers GJ, Spiegelhalder B, Walker R, Wisnok JS. 1994. Nitrate, nitrite and N-nitroso compounds. Eur J Pharmacol 292: 1-38.
  10. Shumilova TE, Nozdrachev AD, Vaĭnshteĭn GB, Semernia VN, Shereshkov VI, Ianvareva IN, Panov AA. 2006. Oxygen consumption in rat brain tissues in conditions of acute nitrite methemoglobinemia. Zh Evol Biokhim Fiziol 42: 63-67.
  11. Avasilcai L, Cuciureanu R. 2011. Nitrates and nitrites in meat products－nitrosamines precursors. Rev Med Chir Soc Med Nat Iasi 115: 606-611.
  12. Sen NP, Smith DC, Schwinghamer L. 1969. Formation of N-nitrosamines from secondary amines and nitrite in human and animal gastric juice. Food Cosmet Toxicol 7: 301-307. https://doi.org/10.1016/S0015-6264(69)80366-4
  13. Chow CK, Hong CB. 2002. Dietary vitamin E and selenium and toxicity of nitrite and nitrate. Toxicology 180: 195-207. https://doi.org/10.1016/S0300-483X(02)00391-8
  14. Hecht SS. 1997. Approaches to cancer prevention based on an understanding of N-nitrosamine carcinogenesis. Proc Soc Exp Biol Med 216: 181-191. https://doi.org/10.3181/00379727-216-44168
  15. Chung MJ, Lee SJ, Choi SY, Sung NJ. 2003. Screening of effective components from kale to inhibit N-nitrosodimethylamine formation. J Korean Soc Food Sci Nutr 32: 223-229. https://doi.org/10.3746/jkfn.2003.32.2.223
  16. Lee SY, Munerol B, Pollard S, Youdim KA, Pannala AS, Kuhnle GG, Debnam ES, Rice-Evans C, Spencer JP. 2006. The reaction of flavanols with nitrous acid protects against N-nitrosamine formation and leads to the formation of nitroso derivatives which inhibit cancer cell growth. Free Radic Biol Med 40: 323-334. https://doi.org/10.1016/j.freeradbiomed.2005.08.031
  17. Cappellani A, Zanghì A, Di Vita M, Cavallaro A, Piccolo G, Veroux P, Lo Menzo E, Cavallaro V, de Paoli P, Veroux M, Berretta M. 2013. Strong correlation between diet and development of colorectal cancer. Front Biosci (Landmark Ed) 18: 190-198. https://doi.org/10.2741/4095
  18. Lin Q, Shen J, Ning Y, Shen S, Das UN. 2013. Inhibitory effects of water caltrop pericarps on the growth of human gastric cancer cells in vitro. Curr Pharm Des 19: 7473-7478.
  19. Zinov'eva VN, Spasov AA. 2012. Mechanisms of plant polyphenols anti-cancer effects. I. Blockade of carcinogenesis initiation. Biomed Khim 58: 160-175. https://doi.org/10.18097/pbmc20125802160
  20. Kurechi T, Kikugawa K, Fukuda S. 1980. Nitrite-reacting substances in Japanese radish juice and their inhibition of nitrosamine formation. J Agric Food Chem 28: 1265-1269. https://doi.org/10.1021/jf60232a071
  21. Raikos N, Fytianos K, Samara C, Samanidou V. 1988. Comparative study of different techniques for nitrate determination in environmental water samples. Fresenius Z Anal Chem 331: 495-498. https://doi.org/10.1007/BF00467037
  22. Yu SY, Lee YJ, Song HS, Hong HD, Lim JH, Choi HS, Lee BY, Kang SN, Lee OH. 2012. Antioxidant effects and nitrite scavenging ability of extract from Acanthopanax cortex shoot. Korean J Food & Nutr 25: 793-799. https://doi.org/10.9799/ksfan.2012.25.4.793
  23. Hotchkiss JH, Barbour JF, Scanlan RA. 1980. Analysis of malted barley for N-nitrosodimethylamine. J Agric Food Chem 28: 678-680. https://doi.org/10.1021/jf60229a025
  24. Sung NJ, Klausner KA, Hotchkiss JH. 1991. Influence of nitrate, ascorbic acid, and nitrate reductase microorganisms on N-nitrosamine formation during Korean-style soysauce fermentation. Food Addit Contam 8: 291-298. https://doi.org/10.1080/02652039109373978
  25. Mao Y, McClements DJ. 2012. Influence of electrostatic heteroaggregation of lipid droplets on their stability and digestibility under simulated gastrointestinal conditions. Food Funct 3: 1025-1034. https://doi.org/10.1039/c2fo30108c
  26. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat Res 113: 173-215. https://doi.org/10.1016/0165-1161(83)90010-9
  27. Walker R. 1975. Naturally occuring nitrate/nitrite in foods. J Sci Food Agric 26: 1735-1742. https://doi.org/10.1002/jsfa.2740261116
  28. Park YS. 1998. Nitrate content and organophosphorus pesticide residues in edible part of organic farming vegetables. J Korean Soc Food Sci Nutr 27: 471-476.
  29. Walker R. 1996. The metabolism of dietary nitrites and nitrates. Biochem Soc Trans 24: 780-785. https://doi.org/10.1042/bst0240780
  30. Sutandyo N. 2010. Nutritional carcinogenesis. Acta Med Indones 42: 36-42.
  31. Anderson LM, Beebe LE, Fox SD, Issaq HJ, Kovatch RM. 1991. Promotion of mouse lung tumors by bioaccumulated polychlorinated aromatic hydrocarbons. Exp Lung Res 17: 455-471. https://doi.org/10.3109/01902149109064432
  32. Cooney RV, Ross PD. 1987. N-nitrosation and N-nitration of morpholine by nitrogen dioxide in aqueous solution: effects of vanillin and related phenols. J Agric Food Chem 35: 789-793. https://doi.org/10.1021/jf00077a036
  33. Pignatelli B, Bereziat JC, Descotes G, Bartsch H. 1982. Catalysis of nitrosation in vitro and in vivo in rats by catechin and resorcinol and inhibition by chlorogenic acid. Carcinogenesis 3: 1045-1049. https://doi.org/10.1093/carcin/3.9.1045
  34. Walker R. 1990. Nitrates, nitrites and N-nitrosocompounds: a review of the occurrence in food and diet and the toxicological implications. Food Addit Contam 7: 717-768. https://doi.org/10.1080/02652039009373938
  35. Lee SJ, Chung MJ, Shin JH, Sung NJ. 2000. Effect of natural plant components on the nitrite-scavenging. J Fd Hyg Safety 15: 88-94.
  36. Tannenbaum SR, Wishnok JS, Leaf CD. 1991. Inhibition of nitrosamine formation by ascorbic acid. Am J Clin Nutr 53: 247S-250S.
  37. Walker EA, Pignatelli B, Friesen M. 1982. The role of phenols in catalysis of nitrosamine formation. J Sci Food Agric 33: 81-88. https://doi.org/10.1002/jsfa.2740330115
  38. Chung FL, Morse MA, Eklind KI, Xu Y. 1993. Inhibition of tobacco-specific nitrosamine-induced lung tumorigenesis by compounds derived from cruciferous vegetables and green tea. Ann NY Acad Sci 686: 186-201. https://doi.org/10.1111/j.1749-6632.1993.tb39174.x
  39. Kuenzig W, Chau J, Norkus E, Holowaschenko H, Newmark H, Mergens W, Conney AH. 1984. Caffeic and ferulic acid as blockers of nitrosamine formation. Carcinogenesis 5: 309-313. https://doi.org/10.1093/carcin/5.3.309
  40. McCann J, Choi E, Yamasaki E, Ames BN. 1975. Detection of carcinogenesis as mutagens in the Salmonella/microsome test: Assay of 300 chemicals. Proc Natl Acad Sci USA 72: 5135-5139. https://doi.org/10.1073/pnas.72.12.5135
  41. Ames BN. 1979. Identifying environmental chemicals causing mutations and cancer. Science 204: 587-593. https://doi.org/10.1126/science.373122
  42. Chung SY, Kim HW, Yoon S. 1999. Analysis of antioxidant nutrients in green yellow vegetable juice. Korean J Food Sci Technol 31: 880-886.
  43. Jeon EJ, Kim JS, Park YK, Kim TS, Kang MH. 2003. Protective effect of yellow-green vegetables juices on DNA damage in Chinese hamster lung cell using comet assay. Korean J Nutr 36: 24-31.
  44. Ahluwalia KS, Roy SK, Chatterjee SS, Swarup V. 1979. Kale - a nutritionally rich vegetable. Indian Horticulture 24: 9-10.
  45. Lee SM, Kim KH, Rhee SH, Park KY. 2000. Antitumorigenic and immunologic effects of kale extracts and chlorophyllin on sarcoma 180 cell-transplanted mice. J Cancer Prev 5: 31-38.
  46. Lee SM, Rhee SH, Park KY. 1993. Antimutagenic effect of soluble dietary fibers from kale and soybean. Environ Mutagen Carcinog 13: 26-35.
  47. Olvera O, Zimmering S, Arceo C, Cruces M. 1993. The protective effects of chlorophyllin in treatment with chromium (VI) oxide in somatic cells of Drosophila. Mutat Res 301: 201-204. https://doi.org/10.1016/0165-7992(93)90079-B
  48. Okuyama T, Takata M, Takayasu J, Hasegawa T, Tokuda H, Nishino A, Nishino H, Iwashima A. 1991. Anti-tumorpromotion by principles obtained from Angelica keiskei. Planta Med 57: 242-246. https://doi.org/10.1055/s-2006-960082
  49. Park WB, Kim DS. 1995. Changes of contents of ${\beta}$-carotene and vitamin C and antioxidative activities of juice of Angelica keiskei Koidz stored at different conditions. Korean J Food Sci Technol 27: 375-379.

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