Molecules and Cells

  • 제37권7호
  • /
  • Pages.554-561
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  • 2014
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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Application of in Utero Electroporation of G-Protein Coupled Receptor (GPCR) Genes, for Subcellular Localization of Hardly Identifiable GPCR in Mouse Cerebral Cortex

  • Kim, Nam-Ho (Department of Pharmacology, College of Medicine, Institute of Natural Medicine, Hallym University) ;
  • Kim, Seunghyuk (Department of Pharmacology, College of Medicine, Institute of Natural Medicine, Hallym University) ;
  • Hong, Jae Seung (Department of Physical Education, Hallym University) ;
  • Jeon, Sung Ho (Department of Life Science and Center for Aging and Health Care, Hallym University) ;
  • Huh, Sung-Oh (Department of Pharmacology, College of Medicine, Institute of Natural Medicine, Hallym University)
  • 투고 : 2014.06.05
  • 심사 : 2014.06.30
  • 발행 : 2014.07.31
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초록

Lysophosphatidic acid (LPA) is a lipid growth factor that exerts diverse biological effects through its cognate receptors ($LPA_1-LPA_6$). $LPA_1$, which is predominantly expressed in the brain, plays a pivotal role in brain development. However, the role of $LPA_1$ in neuronal migration has not yet been fully elucidated. Here, we delivered $LPA_1$ to mouse cerebral cortex using in utero electroporation. We demonstrated that neuronal migration in the cerebral cortex was not affected by the overexpression of $LPA_1$. Moreover, these results can be applied to the identification of the localization of $LPA_1$. The subcellular localization of $LPA_1$ was endogenously present in the perinuclear area, and overexpressed $LPA_1$ was located in the plasma membrane. Furthermore, $LPA_1$ in developing mouse cerebral cortex was mainly expressed in the ventricular zone and the cortical plate. In summary, the overexpression of $LPA_1$ did not affect neuronal migration, and the protein expression of $LPA_1$ was mainly located in the ventricular zone and cortical plate within the developing mouse cerebral cortex. These studies have provided information on the role of $LPA_1$ in brain development and on the technical advantages of in utero electroporation.

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