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Effect of Hot-water Dipping on Inhibiting Red Discoloration of Basal Part in Chicon

열수 침지 처리가 치콘 절단면의 적변 억제에 미치는 영향

  • Jung, Hyun Jin (Department of Horticulture, Kangwon National University) ;
  • Kang, Ho-Min (Department of Horticulture, Kangwon National University)
  • Received : 2013.10.08
  • Accepted : 2014.02.05
  • Published : 2014.06.30

Abstract

Hot water dipping test was conducted for chicon to restrict red discoloration of its basal part which impairs the product value during sales. Hot water dipping treatment was given to chicon for 4 min and for 8 min at $38^{\circ}C$ and for 2 min and 4 min at $42^{\circ}C$, and for 1 min and 2 min at $45^{\circ}C$, along with control (for one min at $20^{\circ}C$). The red discoloration indices of basal part of chicon during sensory evaluation on the sixth day of storage under the storage temperature at $10^{\circ}C$ was lower at $42^{\circ}C$ for 2 min, $42^{\circ}C$ for 4 min and $45^{\circ}C$ for 1 min treatments. The color change value of the basal part in chicon measured by colorimeter showed that the lowest ${\Delta}a^*$ and ${\Delta}h$ were maintained in the basal part of chicon treated at $42^{\circ}C$ for 2 min. Whereas, color changes in $42^{\circ}C$ for 2 min and $45^{\circ}C$ for 1 min treatments were significantly low as compared with that of control. The contents of total phenolic compounds which are the substances that cause red discoloration of basal part in chicon were lowest at $42^{\circ}C$ for 2 min, $42^{\circ}C$ for 4 min and $45^{\circ}C$ for 1 min treatments. The activity of phenylalanine ammonia lyase (PAL) resposible for in the synthesis of phenolic substances was the least in $42^{\circ}C$ for 2 min treatment. Whereas, PAL activity of the chicons treated a t $42^{\circ}C$ for 2 min and at $45^{\circ}C$ for 1 min were significantly lower than that of control. However, red discoloration was progressed as similar level with that of control in the basal part of chicon at $45^{\circ}C$ for 2 min. The contents of total phenolic compounds and PAL activity in this treatment were not significantly different from those in control. The polyphenol oxidase (PPO) activity which causes red discoloration of cut tissues was low in all the treatments including $42^{\circ}C$ and $45^{\circ}C$ treatment at which no inhibition effects of the red discoloration of basal part of chicon were observed. When the correlation coefficient between each investigated index was tested, most of them showed high correlation except the PPO activity and particularly and the red discoloration index and sensory evaluation ${\Delta}h$ values, and PAL activity and total phenolic compounds content were $r=0.927^{**}$, and $r=0.942^{**}$, respectively.

치콘의 판매 중 상품성 저하의 원인이 되는 치콘 절단면적변 현상을 억제하기 위해 열수 침지 실험을 실시하였다. 열수침지처리로는 대조구($20^{\circ}C$ 1분)과 $38^{\circ}C$에서의 4분, 8분, $42^{\circ}C$에서의 2분, 4분, 그리고 $45^{\circ}C$에서의 1분, 2분을 두었다. 색차계로 측정한 ${\Delta}a^*$${\Delta}h$값은 $42^{\circ}C$ 2분 처리에서는 가장 낮게 유지되었으며, $42^{\circ}C$의 2분과 $45^{\circ}C$의 1분 처리구에서는 대조구보다 유의 있는 낮은 값을 나타내었다. $10^{\circ}C$에 저장 6일째 관능 평가한 절단면의 적변지수는 $42^{\circ}C$의 2분과 4분 처리 그리고 $45^{\circ}C$의 1분 처리에서 낮았다. 적변의 원인 물질이 되는 총페놀함량을 저장 6일째 조사하였는데, 이 또한 절단면의 색변화가 적었던 $42^{\circ}C$의 2분과 4분 처리 그리고 $45^{\circ}C$의 1분 처리에서 낮았다. 페놀물질 합성에 관여하는 PAL 활성은 $42^{\circ}C$ 2분 처리에서는 가장 낮은 값을 보였으며, $42^{\circ}C$의 2분와 $45^{\circ}C$의 1분 처리구에서는 대조구보다 유의 있는 낮은 수치를 보였다. 그러나 가장 강한 열처리였던 $45^{\circ}C$의 2분 처리에서는 절단면의 적변이 대조구와 유사한 수준으로 진행되었으며, 페놀함량과 PAL 활성도 대조구와 차이가 없었다. 공기에 노출된 절단조직에서 페놀물질을 산화시켜 적변을 일으키는 PPO의 활성은 $42^{\circ}C$$45^{\circ}C$의 모든 처리구에서 낮았는데, 특히 다른 조사항목에서는 모두 대조구와 유사한 수준을 보였던 $45^{\circ}C$의 2분 처리구가 가장 낮은 수치를 보였다. 또한 각 조사항목간 상관관계에서, PPO를 제외한 모든 조사항목간에서는 대부분 고도의 상관관계를 보였으며, 특히 관능평가한 적변지수와 ${\Delta}h$값은 $r=0.927^{***}$를, PAL 활성과 총 페놀물질 함량과는 $r=0.942^{***}$의 고도의 상관관계를 보였다.

Keywords

References

  1. Bae, J.H., K.W. Park, and H.M. Kang. 2005. Effects of packing materials, light condition and storage temperature on MAP storage of chicon. J. Bio-Environment Cont. 14:67-75.
  2. Berset, C. and P. Caniaux. 1983. Relationship between color evaluation and chlorophyllian pigment content in dried parsley leaves. J. Food Sci. 48:1854-1857. https://doi.org/10.1111/j.1365-2621.1983.tb05100.x
  3. Charles, F., C. Guillaume, and N. Gontard. 2008. Effect of passive and active modified atmosphere packaging on quality changes of fresh endives. Postharvest Biol. Technol. 48:22-29. https://doi.org/10.1016/j.postharvbio.2007.09.026
  4. Castaner, M., I.G. Maria, M.V. Ruiz, and F. Artes. 1999. Browning susceptibility of minimally processed baby and romaine lettuces. Eur. Food Res. Technol. 209:52-56. https://doi.org/10.1007/s002170050456
  5. Hong, S.I., S.M. Son, H.H. Lee, and D.M Kim. 2004. Effect of hot water treatment on storage quality of minimally processed onion. Kor. Food Sci. Technol. 36:239-245.
  6. In, B.C. and J.G. Kim. 2008. Effect of precooling and harvesting at different times on respiration, browning and microbial growth of fresh-cut iceberg lettuce. Kor. J. Hort. Sci. Technol. 26:258-264.
  7. Jung, H.J., H.T. Seo, I.L Choi, T.J. Yoo, J.S. Son, J.H. Won, I.S. Kim, and H.M. Kang. 2010. Effect of Precooling Treatments on the Storability of Chicon during MA storage. J. Bio-Environment Cont. 19(4):360-365.
  8. Kavrayan, D. and T. Aydemir. 2001. Partial purification and characterization of polyphenol oxidase from peppermint (Mentha piperita). Food Chem. 74:147-154. https://doi.org/10.1016/S0308-8146(01)00106-6
  9. Kang, H.M. and M.E. Saltveit. 2003. Wound-induced PAL activity is suppressed by heat-shock treatments that induce the synthesis of heat-shock proteins. Physiologia Plantarum 119:450-455. https://doi.org/10.1034/j.1399-3054.2003.00190.x
  10. Ke, D. and M.E. Saltveit. 1989. Wound-induced ethylene production, phenolic metabolism and susceptibility to russet spotting in iceberg lettuce. Physiol. Plant. 76:412-418. https://doi.org/10.1111/j.1399-3054.1989.tb06212.x
  11. Loaiza-Velarde, J.G., F.A. Tomas-Barberan, and M.E. Saltveit. 1997. Effect of intensity and duration of heat-shock treatments on wound-induced phenolic metabolism in iceberg lettuce. J. Am. Soc. Hortic. Sci. 122:873-877.
  12. Luh, B.S. and B. Phithakpol. 1972. Characteristics of polyphenoloxidase related to browning in cling peaches. J. Food Sci. 37:264-268. https://doi.org/10.1111/j.1365-2621.1972.tb05832.x
  13. Park, K.W. 1994. Western vegetable. Korea Univ. Publishing, Seoul, Korea. p. 271-281.
  14. Peiser, G., G. Lopez-Galvez, M. Cantwell, and M.E. Saltveit. 1998. Phenylalanine ammonia lyase inhibitors control browning of cut lettuce. Postharvest Biol. Technol. 14:171-177. https://doi.org/10.1016/S0925-5214(98)00048-9
  15. Rivero, R.M., J.M. Ruiz, P.C. Garcia, L.R. Lopez-Lefebre, E. Sanchez, and L. Romero. 2001. Resistance to cold and heat stress: Accumulation of phenolic compounds in tomato and watermelon plants. Plant Sci. 160:315-321. https://doi.org/10.1016/S0168-9452(00)00395-2
  16. Rodriguez-Lopez, J.N., L.G. Fenoll, M.J. Penalver, P.A. Garcia- Ruiz, R. Varon, F. Martinez-Ortiz, and F. Garcia-Canovas. 2001. Tyrosinase action on monophenols; Evidence for direct enzymatic release of o-diphenol. Biochemica Biophysica Acta. 1548:238-256. https://doi.org/10.1016/S0167-4838(01)00237-0
  17. Saltveit, M.E. 2000. Wound induced changes in phenolic metabolism and tissue browning are altered by heat shock. Postharvest Biol. Technol. 21:61-69. https://doi.org/10.1016/S0925-5214(00)00165-4
  18. Saltveit, M.E. and L. Qin. 2008. Heating the ends of leaves cut during coring of whole heads of lettuce reduces subsequent phenolic accumulation and tissue browning. Postharvest Biol. Technol. 47:255-259. https://doi.org/10.1016/j.postharvbio.2007.06.021
  19. Salman, A., P. Goupil, H. Filgueiras, F. Charles, G. Ledoigt, and H. Sallanon. 2008. Controlled atmosphere and heat shock aVect PAL1 and HSP90 mRNA accumulation in fresh-cut endive (Cichorium intybus L.). Eur. Food Res. Technol. 227: 721-726. https://doi.org/10.1007/s00217-007-0778-6
  20. Takahashi, T., K. Abe, and K. Chachin. 1996. Studies on the physiological and chemical changes in shredded cabbage, 3: Effect of air-exposure at low temperature on physiological activities and browning of shredded cabbage. J. Japan. Soc. Food Sci. Technol. 43:663-667. https://doi.org/10.3136/nskkk.43.663
  21. Van Gelder, C.W.G., W.H. Flurkey, and H. Wichers. 1997. Sequence and structural features of plant and fungal tyrosinases. Phytochem. 45:1309-1323. https://doi.org/10.1016/S0031-9422(97)00186-6
  22. Vanstreels, E., J. Lammertyn, B.E. Verlinden, N. Gillis, A. Schenk, and B.M. Nicolai. 2002. Red discoloration of chicory under controlled atmosphere conditions. Postharvest Biol. Technol. 26:313-322. https://doi.org/10.1016/S0925-5214(02)00060-1