Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Diversity and Physiological Characteristics of Culturable Bacteria from Marine Sediments of Ross Sea, Antarctica

남극 로스해 퇴적물로부터 분리된 세균의 다양성 및 생리학적 특성

  • Lee, Yung Mi (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Jung, You-Jung (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Hong, Soon Gyu (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Kim, Ji Hee (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Lee, Hong Kum (Division of Polar Life Sciences, Korea Polar Research Institute)
  • 이영미 (극지연구소 극지생명과학연구부) ;
  • 정유정 (극지연구소 극지생명과학연구부) ;
  • 홍순규 (극지연구소 극지생명과학연구부) ;
  • 김지희 (극지연구소 극지생명과학연구부) ;
  • 이홍금 (극지연구소 극지생명과학연구부)
  • Received : 2014.03.14
  • Accepted : 2014.04.02
  • Published : 2014.06.30
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Abstract

The affiliations and physiological characteristics of culturable bacteria isolated from the sediments of Ross Sea, Antarctica were investigated. Sixty-three isolates obtained by cultivation were grouped into 21 phylotypes affiliated with the phyla Actinobacteria and Bacteroidetes and with the classes Alphaproteobacteria and Gammaproteobacteria by phylogenetic analysis of 16S rRNA gene sequences. Based on phylogenetic analysis (<98.65% sequence similarity), approximately 49% of total isolates represented potentially novel species or genus. Among them, extracellular protease, lipase, and exopolysaccharide activities at $10^{\circ}C$ or $20^{\circ}C$ were detected in approximately 46%, 25%, and 32% of the strains, respectively. Forty-three isolates produced at least one type of extracellular material and 21 of them produced at least two extracellular protease, lipase, and/or exopolysaccharides. Our findings indicate that culturable bacterial diversity present within the marine sediments of Ross Sea, Antarctica may contribute to the hydrolysis of the major organic constituents which is closely related with carbon and nitrogen cycling in this environment.

남극 로스해의 퇴적물로부터 배양을 통해 분리한 균주의 분류 및 생리학적 특성 분석을 수행하였다. 분리 세균 63균주의 16S rRNA 유전자 염기서열을 이용한 계통분류학적 분석 결과, 이들은 Actinobacteria, Bacteroidetes, Alphaproteobacteria 및 Gammaproteobacteria 내의 21개의 파일로타입(phylotypes)에 속하였다. 98.65% 염기서열 유사도를 기준으로, 약 49%의 균주가 잠재적으로 신종 또는 신속 후보인 것으로 나타났다. 분리된 균주 중, 각각 46%, 25% 및 32%의 균주가 세포외 단백질분해효소, 지질분해효소 및 외부다당체 생성에 대한 활성을 나타냈다. 43개의 균주는 최소 1개의 세포외 분비 물질을 생산하였고, 이들 중 21개 균주는 최소 2개의 세포외 단백질분해효소, 지질분해효소 또는/및 세포외다당체를 생성하였다. 이러한 결과는 남극 로스해 퇴적물 내의 배양된 세균 군집이 해당 환경에서 탄소와 질소와 관련된 유기물질의 가수분해에 영향을 미치고 있다는 것을 시사한다.

Keywords

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