Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Immune and Anti-oxidant Functions of Ethanol Extracts of Scutellaria baicalensis Georgi in Mice Bearing U14 Cervical Cancers

  • Peng, Yong (Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University) ;
  • Guo, Cong-Shan (Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University) ;
  • Li, Pan-Xia (Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University) ;
  • Fu, Zhan-Zhao (Department of Oncology, the First Hospital of Qinhuangdao City) ;
  • Gao, Li-Ming (Department of Oncology, the First Hospital of Qinhuangdao City) ;
  • Di, Ya (Department of Oncology, the First Hospital of Qinhuangdao City) ;
  • Ju, Ya-Kun (Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University) ;
  • Tian, Ru (Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University) ;
  • Xue, Jia-Jia (Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University)
  • Published : 2014.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: The objective was to study the effect of Scutellaria baicalensis Georgi ethanol extracts (SBGE) on immune and anti-oxidant function in U14 tumor-bearing mice. Materials and Methods: U14 tumor-bearing mice were randomly divided into eight groups: a control group, a cyclophosphamide (CTX) group, three dose groups of SBGEI (high, medium, low), and three dose groups of SBGEII (high, medium, low). After two weeks, the thymus and spleen weight indices of mice bearing U14 cervical cancer were calculated. Enzyme linked immunosorbent assays (ELISA) was used to determine the levels of serum IL-2, TNF-${\alpha}$, IL-8, and PCNA. MDA activity and SOD activity in plasma were measured with detection kits. Results: In the SBGE groups, thymus weight and spleen weight indices of U14 tumor-bearing mice were significantly higher than in the control group or CTX group (p<0.05). Compared to control group, the levels of serum IL-2 and TNF-${\alpha}$ in U14 tumor-bearing mice increased significantly, whereas the contents of serum IL-8 and PCNA decreased (p<0.05). The activity of SOD increased with the growing dose of SBGE, while the activity of MDA decreased significantly in the highe-rdose groups of SBGE. Conclusions: These findings suggested that SBGE, especially at high dose, 1000 mg/kg, showed significant immune and anti-oxidant effects infU14 tumor-bearing mice, which might be the mechanisms of SBGE inhibition of tumor growth.

Keywords

References

  1. Belardelli F, Ferrantini M (2002). Cytokines as a link between innate and adaptive antitumor immunity. Trends Immunol, 23, 201-8. https://doi.org/10.1016/S1471-4906(02)02195-6
  2. Bosch FX, Manos MM, Manoz N (1995). Relevance of human papilloma virus in cervical cancer: a worldwide perspective. J Natl Cancer Invest, 87, 796-9. https://doi.org/10.1093/jnci/87.11.796
  3. Branca M, Ciotti M, Giorgi C, et al (2007). Up-regulation of proliferating cell nuclear antigen (PCNA) is closely associated with high-risk human papillomavirus (HPV) and progression of cervical intraepithelial neoplasia (CIN), but does not predict disease outcome in cervical cancer. Eur J Obstet Gynecol Reprod Biol, 130, 223-31. https://doi.org/10.1016/j.ejogrb.2006.10.007
  4. Jeong K, Shin YC, Park S, et al (2011). Ethanol extract of Scutellaria baicalensis Georgi prevents oxidative damage and neuroinflammation and memorial impairments in artificial senescense mice. J Biomed Sci, 18, 14-26. https://doi.org/10.1186/1423-0127-18-14
  5. Kesarkar S, Bhandage A, Deshmukh S, et al (2009). Flavonoids: an overview. J Pharm Res, 2, 1148-54.
  6. Lin MG, Liu LP, Li CY, et al (2013). Scutellaria extract decreases the proportion of side population cells in a myeloma cell Line by down-regulating the expression of ABCG2 protein. Asian Pac J Cancer Prev, 14, 7179-86. https://doi.org/10.7314/APJCP.2013.14.12.7179
  7. Lu Y, Joerger R, Wu C (2011). Study of the chemical composition and antimicrobial activities of ethanolic extracts from roots of Scutellaria baicalensis Georgi. J Agric Food Chem, 59, 10934-42. https://doi.org/10.1021/jf202741x
  8. Luppi F, Longo AM, de Boer WI, et al (2007). Interleukin-8 stimulates cell proliferation in non-small cell lung cancer through epidermal growth factor receptor transactivation. Lung Cancer, 56, 25-33. https://doi.org/10.1016/j.lungcan.2006.11.014
  9. Mauceri HJ, Seetharam S, Beckett MA, et al (2002). Tumor production of angiostatin is enhanced after exposure to TNF-$\alpha$. Int J Cancer, 97, 410-5. https://doi.org/10.1002/ijc.1629
  10. Mingari MC, Gerosa F, Carra G, et al (1984). Human interleukin-2 promotes proliferation of activated B cells via surface receptors similar to those of activated T cells. Nature, 312, 641-3. https://doi.org/10.1038/312641a0
  11. Oaknin A, Diaz de Corcuera I, Rodriguez-Freixinos V, et al (2012). SEOM guidelines for cervical cancer. Clin Transl Oncol, 14, 516-9. https://doi.org/10.1007/s12094-012-0834-y
  12. Overwijk WW, Theoret MR, Restifo NP (2000). The future of interleukin-2: enhancing therapeutic anticancer vaccines. Cancer J Sci Am, 6, 76-80.
  13. Peng Y, Sun M, Di Y, et al (2012). Antitumor effect of the ethanol extract of Scutellaria baicalensis on the mice bearing U14 cervical cancer. Afr J Biotechnol, 11, 6542-9.
  14. Su S, He CM, Li LC, et al (2008). Genetic characterization and phytochemical analysis of wild and cultivated populations of Scutellaria baicalensis. Chem Biodivers, 5, 1353-63. https://doi.org/10.1002/cbdv.200890123
  15. Valko M, Rhodes CJ, Moncol J, et al (2006). Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact, 160, 1-40. https://doi.org/10.1016/j.cbi.2005.12.009
  16. van Etten B, de Vries MR, van Ijken MG, et al (2003). Degree of tumour vascularity correlates with drug accumulation and tumour response upon TNF-alpha-based isolated hepatic perfusion. Br J Cancer, 88, 314-9. https://doi.org/10.1038/sj.bjc.6600707
  17. Wang JL, Zheng BY, Li XD, et al (2004). Predictive significance of the alterations of p16INK4A, p14ARF, p53, and proliferating cell nuclear antigen expression in the progression of cervical cancer. Clin Cancer Res, 10, 2407-14. https://doi.org/10.1158/1078-0432.CCR-03-0242
  18. Wang YY, Li L, Wei S, et al (2013). Human papillomavirus (HPV) infection in women participating in cervical cancer screening from 2006 to 2010 in Shenzhen City, South China. Asian Pac J Cancer Prev, 14, 7483-7. https://doi.org/10.7314/APJCP.2013.14.12.7483
  19. Waugh DJ, Wilson C (2008). The interleukin-8 pathway in cancer. Clin Cancer Res, 14, 6735-41. https://doi.org/10.1158/1078-0432.CCR-07-4843
  20. Yao C, Lin Y, Chua MS, et al (2007). Interleukin-8 modulates growth and invasiveness of estrogen receptor-negative breast cancer cells. Int J Cancer, 121, 1949-57. https://doi.org/10.1002/ijc.22930
  21. Zhang M, Cai S, Shi D (1999). Prognostic value of cell proliferation and apoptosis in uterine cervical cancer treated with radiation. Zhonghua Zhong Liu Za Zhi, 21, 290-2.

Cited by

  1. Anti-tumor effects of Abnormal Savda Munziq on the transplanted cervical cancer (U27) mouse model vol.16, pp.1, 2016, https://doi.org/10.1186/s12906-016-1458-5
  2. Synergistic cytotoxic effects of a combined treatment of a Pinellia pedatisecta lipid-soluble extract and cisplatin on human cervical carcinoma in vivo vol.13, pp.6, 2017, https://doi.org/10.3892/ol.2017.6091
  3. MicroRNA-92a promotes tumor growth and suppresses immune function through activation of MAPK/ERK signaling pathway by inhibiting PTEN in mice bearing U14 cervical cancer vol.7, pp.7, 2018, https://doi.org/10.1002/cam4.1329