Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Antiproliferative Effects of Celecoxib in Hep-2 Cells through Telomerase Inhibition and Induction of Apoptosis

  • Zhao, Yong-Qiang (Department of Otolaryngology & Head and Neck Surgery, the Second Hospital of Shandong University) ;
  • Feng, Hui-Wei (Department of Otolaryngology & Head and Neck Surgery, the Second Hospital of Shandong University) ;
  • Jia, Tao (Department of Otolaryngology & Head and Neck Surgery, the Second Hospital of Shandong University) ;
  • Chen, Xue-Mei (Department of Otolaryngology & Head and Neck Surgery, the Second Hospital of Shandong University) ;
  • Zhang, Hui (Department of Otolaryngology & Head and Neck Surgery, the Second Hospital of Shandong University) ;
  • Xu, An-Ting (Department of Otolaryngology & Head and Neck Surgery, the Second Hospital of Shandong University) ;
  • Zhang, Hai-Ling (Department of Otolaryngology & Head and Neck Surgery, the Second Hospital of Shandong University) ;
  • Fan, Xian-Liang (Department of Otolaryngology & Head and Neck Surgery, the Second Hospital of Shandong University)
  • Published : 2014.06.30
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Abstract

Background: To investigate the effect of celecoxib on telomerase activity and apoptosis in a human laryngeal squamous carcinoma cell line (Hep-2 cells). Materials and Methods: The growth inhibition rate of Hep-2 cells in vitro was measured by MTT assay, and apoptosis by TUNEL assay and flow cytometry (FCM). The TRAP-ELISA method was used to determine telomerase activity in Hep-2 cells. The mRNA expression of human telomerase RNA component(hTR), human telomerase reverse transcriptase (hTERT) and human telomerase-associated protein(hTEP1) was determined by RT-PCR assay. Expression of Bax and Bcl-2 proteins was assessed by Western blotting. Results: Celecoxib can inhibit proliferation and induce apoptosis in a dose- and time-dependent manner, repress telomerase activity, decrease hTERT mRNA and Bcl-2 protein expression and increase Bax protein expression, PGE2 had no effect on telomerase. Conclusions: Celecoxib had the antiproliferative and pro-apoptotic effect in Hep-2 cells. Apoptosis was accompanied by a decrease in telomerase activity which was directly correlated with hTERT mRNA and up-regulation of Bax/Bcl-2. Bcl-2 may thus play an important role in telomerase activity as well as apoptosis.

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