Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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MiR-421 Regulates Apoptosis of BGC-823 Gastric Cancer Cells by Targeting Caspase-3

  • Wu, Jian-Hong (Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University) ;
  • Yao, Yong-Liang (Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University) ;
  • Gu, Tao (Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University) ;
  • Wang, Ze-You (Institute of Cancer Research, Central South University) ;
  • Pu, Xiong-Yong (Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University) ;
  • Sun, Wang-Wei (Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University) ;
  • Zhang, Xian (Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University) ;
  • Jiang, Yi-Biao (Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University) ;
  • Wang, Jian-Jun (Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to JiangSu University)
  • Published : 2014.07.15
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Abstract

MicroRNAs might act as oncogenes or tumor suppressors in cancer. Recent studies have shown that miR-421 is up-regulated in human gastric cancer. Here, we found that miR-421 was over-expressed in gastric cancer tissues and cell lines. Bioinformatics analysis predicted that the caspase-3 gene was a target of miR-421. Caspase-3 was negatively regulated by miR-421 at the post-transcriptional level. Bax and Bcl-2 were also regulated by miR-421. Moreover, tumor necrosis factor receptor-I and -II, death receptors in the apoptosis pathway, were up-regulated by miR-421. The over-expression of miR-421 promoted gastric cancer cell growth and inhibited apoptosis of the BGC-823 gastric cancer cell line. These observations indicate that miR-421 acts as a tumor promoter by targeting the caspase-3 gene and preventing apoptosis of gastric cancer cells through inhibition of caspase-3 expression. These findings contribute to our understanding of the functions of miR-421 in gastric cancer.

Keywords

References

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