Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Evaluation of the Atlas Helicobacter pylori Stool Antigen Test for Diagnosis of Infection in Adult Patients

  • Osman, Hussein Ali (Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia) ;
  • Hasan, Habsah (Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia) ;
  • Suppian, Rapeah (Department of Biomedical Science, School of Health Sciences, Universiti Sains Malaysia) ;
  • Bahar, Norhaniza (Department of Medicine, Hospital Kuala Lumpur) ;
  • Che Hussin, Nurzam Suhaila (Department of Pathology, Hospital Kuala Lumpur) ;
  • Rahim, Amry Abdul (Department of Medicine, School of Medical Sciences, Universiti Sains Malaysia) ;
  • Hassan, Syed (Department of Surgery, School of Medical Sciences, Universiti Sains Malaysia) ;
  • Andee, Dzulkarnaen Zakaria (Department of Surgery, School of Medical Sciences, Universiti Sains Malaysia) ;
  • Zilfalil, Bin-Alwi (Department of Paediatrics, School of Medical Sciences, Universiti Sains Malaysia)
  • Published : 2014.07.15
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Abstract

Background: Helicobacter pylori (H.pylori) is one of the most important causes of dyspepsia and gastric cancer and diagnosis can be made by invasive or non-invasive methods. The Atlas Helicobacter pylori antigen test is a new rapid non-invasive method which is simple to conduct. The aim of this study was to determine its sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy. Materials and Methods: This prospective study was conducted between July 2012 and December 2013. Stool samples of 59 dyspeptic patients who underwent upper endoscopy were evaluated for H. pylori stool antigen. Results: From the 59 patients who participated in this study, there were 36 (61%) males and 23 (39%) females. H. pylori was diagnosed in 24 (40.7%) gastric biopsies, 22 (91.7 %) of these being positive for the Atlas H. pylori antigen test. The sensitivity, specificity, PPV, NPV and accuracy were 91.7%, 100%, 100%, 94.6% and 96.6% respectively. Conclusions: The Atlas H. pylori antigen test is a new non-invasive method which is simple to perform and avails reliable results in a few minutes. Thus it can be the best option for the diagnosis of H. pylori infection due to its high sensitivity and specificity.

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References

  1. Al-Humayed SM, Ahmed ME, Bello CS, Tayyar MA (2008). Comparison of 4 laboratory methods for detection of Helicobacter pylori. Saudi Med J, 29, 530-2.
  2. Amjad N, Osman HA, Razak NA, et al (2010). Clinical significance of Helicobacter pylori cagA and iceA genotype status. World J Gastroenterol, 16, 4443-7. https://doi.org/10.3748/wjg.v16.i35.4443
  3. Ben Mansour K, Fendri C, Zribi M, et al (2010). Prevalence of Helicobacter pylori vacA, cagA, iceA and oipA genotypes in Tunisian patients. Ann Clin Microbiol Antimicrob, 9, 10. https://doi.org/10.1186/1476-0711-9-10
  4. Bhewa Y, Hilmi I, Cheah PL, Navaratnam P, Goh KL (2007). Evaluation of the monoclonal stool antigen test for Helicobacter pylori in an Asian population with dyspepsia. J Dig Dis, 8, 207-10. https://doi.org/10.1111/j.1751-2980.2007.00307.x
  5. Brown LM, Thomas TL, Ma JL, et al (2002). Helicobacter pylori infection in rural China: demographic, lifestyle and environmental factors. Int J Epidemiol, 31, 638-45. https://doi.org/10.1093/ije/31.3.638
  6. Ceken N, Yurtsever SG, Baran N, et al (2011). Comparison of Helicobacter pylori antibody detection in stool with other diagnostic tests for infection. Asian Pac J Cancer Prev, 12, 1077-81.
  7. Ghotaslou R, Milani M, Akhi MT, et al (2013). Diversity of Helicobacter pylori cagA and vacA genes and its relationship with clinical outcomes in Azerbaijan, Iran. Adv Pharm Bull, 3, 57-62.
  8. Gisbert JP, Pajares JM (2004). Stool antigen test for the diagnosis of Helicobacter pyloriinfection: a systematic review. Helicobacter, 9, 347-68. https://doi.org/10.1111/j.1083-4389.2004.00235.x
  9. Jekarl DW, An YJ, Lee S, et al (2013). Evaluation of a newly developed rapid stool antigen test using an immunochromatographic assay to detect Helicobacter pylori. Jpn J Infect Dis, 66, 60-4. https://doi.org/10.7883/yoken.66.60
  10. Kalem F, Ozdemir M, Baysal B (2010). Investigation of the presence of Helicobacter pylori by different methods in patients with dyspeptic complaints. Mikrobiyol Bul, 44, 29-34.
  11. Khalilpour A, Santhanam A, Wei LC, et al (2013). Antigenic proteins of Helicobacter pyloriof potential diagnostic value. Asian Pac J Cancer Prev, 14, 1635-42. https://doi.org/10.7314/APJCP.2013.14.3.1635
  12. Manes G, Balzano A, Iaquinto G, et al (2001). Accuracy of the stool antigen test in the diagnosis of Helicobacter pyloriinfection before treatment and in patients on omeprazole therapy. Aliment Pharmacol Ther, 15, 73-9. https://doi.org/10.1046/j.1365-2036.2001.00907.x
  13. Miernyk K, Morris J, Bruden D, et al (2011). Characterization of Helicobacter pyloricagA and vacA genotypes among Alaskans and their correlation with clinical disease. J Clin Microbiol, 49, 3114-21. https://doi.org/10.1128/JCM.00469-11
  14. Peng NJ, Lai KH, Lo GH, Hsu PI (2009). Comparison of noninvasive diagnostic tests for Helicobacter pylori infection. Med Princ Pract, 18, 57-61. https://doi.org/10.1159/000163048
  15. Pourakbari B, Mirsalehian A, Maleknejad P, et al (2011). Evaluation of a new antigen for diagnosis of Helicobacter pylori infection in stool of adult and children. Helicobacter, 16, 42-6. https://doi.org/10.1111/j.1523-5378.2010.00813.x
  16. Redeen S, Petersson F, Tornkrantz E, et al (2011). Reliability of diagnostic tests for Helicobacter pylori infection. Gastroenterol Res Pract, 2011, 940650.
  17. Ricci C, Holton J, Vaira D (2007). Diagnosis of Helicobacter pylori: invasive and non-invasive tests. Best Pract Res Clin Gastroenterol, 21, 299-313. https://doi.org/10.1016/j.bpg.2006.11.002
  18. Shimoyama T, Kato C, Kodama M, Kobayashi I, Fukuda Y (2009). Applicability of a monoclonal antibody-based stool antigen test to evaluate the results of Helicobacter pylori eradication therapy. Jpn J Infect Dis, 62, 225-7.
  19. Silva JM, Villares CA, Monteiro Mdo S, et al (2010). Validation of a rapid stool antigen test for diagnosis of Helicobacter pyloriinfection. Rev Inst Med Trop Sao Paulo, 52, 125-8. https://doi.org/10.1590/S0036-46652010000300002
  20. Tan VP, Wong BC (2011). Helicobacter pylori and gastritis: Untangling a complex relationship 27 years on. J Gastroenterol Hepatol, 26, 42-5.
  21. Valliani A, Khan F, Chagani B, et al (2013). Factors associated with Helicobacter pylori infection, results from a developing country - Pakistan. Asian Pac J Cancer Prev, 14, 53-6. https://doi.org/10.7314/APJCP.2013.14.1.53
  22. Zhang M, Zhou YZ, Li XY, et al (2014). Seroepidemiology of Helicobacter pylori infection in elderly people in the Beijing region, China. World J Gastroenterol, 20, 3635-9. https://doi.org/10.3748/wjg.v20.i13.3635
  23. Zhao Y, Wang J, Tanaka T, et al (2012). Association between HLA-DQ genotypes and haplotypes vs Helicobacter pylori infection in an Indonesian population. Asian Pac J Cancer Prev, 13, 1247-51. https://doi.org/10.7314/APJCP.2012.13.4.1247

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