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Effects of Quercetin and Genistein on Boar Sperm Characteristics and Porcine IVF Embyo Developments

  • Kim, Tae-Hee (College of Animal Life Sciences, Kangwon National University) ;
  • Yuh, In-Suh (College of Animal Life Sciences, Kangwon National University) ;
  • Park, In-Chul (School of Veterinary, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary, Kangwon National University) ;
  • Kim, Jong-Taek (School of Veterinary, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University)
  • 투고 : 2014.04.01
  • 심사 : 2014.05.15
  • 발행 : 2014.06.30

초록

Quercetin and genistein, plentifully present in fruits and vegetables, are flavonoid family members that have antioxidative function and plant-derived phytoestrogen activity. The antioxidative effects of quercetin and genistein on boar sperm characteristics and in vitro development of IVF embryo were investigated. The sperm motility was increased by addition of genistein $50{\mu}M$ for 6 hr incubation compared to control (p<0.05). The sperm viability was increased by addition of quercetin 1 and $50{\mu}M$ and genestein 1 and $50{\mu}M$ for 3 hr incubation. In addition, the sperm viability seemed to be increased dose-dependantly by addition of quercetin or genistein 1 and $50{\mu}M$, respectively (p<0.05). The membrane integrities were not increased by quercetin or genistein treatments for 3 hr or 6 hr incubation period except for quercetin $1{\mu}M$ for 3 hr incubation. In mitochondrial activities, addition of quercetin $50{\mu}M$ for 6 hr incubation increased mitochondrial activity but decreased at $100{\mu}M$ concentration compared with control (p<0.05). When porcine IVF embryos were cultured in PZM-3 medium supplemented with low concentrations of quercetin ($1{\sim}10{\mu}M$), the developmental rates to morula and blastocyst increased but significantly decreased at high concentrations of quercetin ($25{\sim}50{\mu}M$). The highest developmental rate to blastocysts among all concentrations of quercetin was shown at quercetin $10{\mu}M$ (p<0.05). The developmental rates to morula or blastocysts at low ($0.01{\sim}1{\mu}M$) and high ($5{\sim}10{\mu}M$) concentrations of genistein were not significantly different among all treatment group and genistein did not affect on IVF embryo development. These results suggest that quercetin and genistein seem to have positive effects at certain concentrations on sperm characteristics such as motility, viability and mitochondrial activity. In addition, low concentrations of quercetin (1, 5 and $10{\mu}M$) in this experiment, seem to have beneficial effect on porcine IVF embryo development but genistein did not affect on it at all given concentrations ($0.01{\sim}10{\mu}M$).

키워드

참고문헌

  1. Aitken RJ. 1994. A free radical theory of male infertility. Reprod. Fertil. Dev. 6(1): 19-23.
  2. Ali AA, Bilodeau JF and Sirard MA. 2003. Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development. Theriogenology 59(3-4): 939-949. https://doi.org/10.1016/S0093-691X(02)01125-1
  3. Alvarez JG and Storey BT. 1995. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol. Reprod. Dev. 42(3): 334-346.
  4. Bain NT, Madan P and Betts DH. 2011. The early embryo respeonse to intracellular reactive oxygen species is developmentally regulated. Reprod. Fertil. Dev. 23(4): 561-575. https://doi.org/10.1071/RD10148
  5. Bennetts LE, De Iuliis GN, Nixon B, Kime M, Zelski K, Mc- Vicar CM, Lewis SE and Aitken RJ. 2008. Impact of estrogenic compounds on DNA integrity in human spermatozoa: evidence for cross-linking and redox cycling activities. Mutat. Res. 641(1-2): 1-11. https://doi.org/10.1016/j.mrfmmm.2008.02.002
  6. Bingham SA, Atkinson C, Liggins J, Bluck L and Coward A. 1998. Phyto-oestrogens: where are we now? Br. J. Nutr. 79(5): 393-406. https://doi.org/10.1079/BJN19980068
  7. Brezezinska-Slebodzinska E, Slebodzinski AB, Pietras B and Wieczorek G. 1995. Antioxidant effect of vitamin E and glutathione on lipid peroxidation in boar semen plasma. Biol. Trace. Elem. Res. 47(1-3): 69-74. https://doi.org/10.1007/BF02790102
  8. Cerolini S, Maldjian A, Surai P and Noble R. 2000. Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Anim. Reprod. Sci. 58 (1-2): 99-111. https://doi.org/10.1016/S0378-4320(99)00035-4
  9. Cordoba M, Santa-Coloma TA, Beorlegui NB and Beconi MT. 1997. Intracellular calcium variation in heparin-capacitated bovine sperm. Biochem. Mol. Biol. Int. 41(4): 725-733.
  10. Coskun O, Kanter M, Korkmaz A and Oter S. 2005. Quercetin, a flavonoid antioxidant, prevents and protects streptozotocininduced oxidative stress and beta-cell damage in rat pancreas. Pharmacol. Res. 51(2): 117-123. https://doi.org/10.1016/j.phrs.2004.06.002
  11. Fraser LR, Abeydeera LR and Niwa K. 1995. Ca(2+)-regulating mechanisms that modulate bull sperm capacitation and acrosomal exocytosis as determined by chlortetracycline analysis. Mol. Reprod. Dev. 40(2): 233-241. https://doi.org/10.1002/mrd.1080400213
  12. Guthrie HD, Welch GR and Long JA. 2008. Mitochondrial function and reactive oxygen species action in relation to boar motility. Theriogenology 70(8): 1209-1215. https://doi.org/10.1016/j.theriogenology.2008.06.017
  13. Jang HY, Jin HA, Lee HY, Kim DJ, Cheong HT, Kim JT, Park IC, Park CK and Yang BK. 2009. Curcumin and vit. E alleviate alone or synergetically hydrogen peroxide inducedoxidative stress on boar sperm characteristics during in vitro storage. Reprod. Dev. Biol. 33(4): 273-281.
  14. Jeong YJ, Kim MK, Song HJ, Kang EJ, Ock SA, Kumar BM, Balasubramanian, S and Rho GJ. 2009. Effect of alphatocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes. Cryobiology 58(2): 181-189. https://doi.org/10.1016/j.cryobiol.2008.12.004
  15. Johnson MK and Loo G. 2000. Effects of epigallocatechin gallate and quercetin on oxidative damage to cellular DNA. Mutat. Res. 459(3): 211-218. https://doi.org/10.1016/S0921-8777(99)00074-9
  16. Khaki A, Fathiazad F, Nouri M, Khak IA, Maleki NA, Khamnei HJ and Ahmadi P. 2010. Beneficial effects of quercetin on sperm parameters in streptozotocin-induced diabetic male rats. Phytother. Res. 24(9): 1285-1291. https://doi.org/10.1002/ptr.3100
  17. Khanduja KL, Verma A and Bhardwaj A. 2001. Impairment of human sperm motility and viability by quercetin is independent of lipid peroxidation. Andrologia 33(5): 277-281. https://doi.org/10.1046/j.1439-0272.2001.00432.x
  18. Laughton MJ, Evans PJ, Moroney MA, Hoult JR and Halliwell B. 1991. Inhibition of mammalian 5-lipoxygenase and cyclooxygenase by flavonoids and phenolic dietary additives. Relationship to antioxidant activity and to iron ion-reducing ability. Biochem. Pharmacol. 42(9): 1673-1681. https://doi.org/10.1016/0006-2952(91)90501-U
  19. Liu D, Jiang H and Grange RW. 2005. Genistein activates the 3',5'-cyclic adenosine monophosphate signaling pathway in vascular endothelial cells and protects endothelial barrier function. Endocrinology 146(3): 1312-1320. https://doi.org/10.1210/en.2004-1221
  20. Mi Y, Zhang C and Taya K. 2007. Quercetin protects spermatogonial cells from 2,4-d-induced oxidative damage in embryonic chickens. J. Reprod. Dev. 53(4): 749-754. https://doi.org/10.1262/jrd.19001
  21. Mitchell JH, Cawood E, Kinniburgh D, Provan A, Collins AR and Irvine DS. 2001. Effect of a phytoestrogen food supplement on reproductive health in normal males. Clin. Sci. (Lond). 100(6): 613-618. https://doi.org/10.1042/CS20000212
  22. Sierens J, Hartley JA, Campbell MJ, Leathem AJ and Woodside JV. 2001. Effect of phytoestrogen and antioxidant supplementation on oxidative DNA damage assessed using the comet assay. Mutat. Res. 485(2): 169-176. https://doi.org/10.1016/S0921-8777(00)00069-0
  23. Takahashi M, Keicho K, Takahashi H, Ogawa H, Schutz RM and Okano A. 2000. Effect of oxidative stress on development and DNA damage in in-vitro cultured bovine embryos by comet assay. Theriogenology. 54(1): 137-145. https://doi.org/10.1016/S0093-691X(00)00332-0
  24. Tou JC, Chen J and Thompson LU. 1998. Flaxseed and its lignan precursor, secoisolariciresinol diglycoside, affect pregnancy outcome and reproductive development in rats. J. Nutr. 128(11): 1861-1868. https://doi.org/10.1093/jn/128.11.1861
  25. Wei H, Wei L, Frenkel K, Bowen R and Barnes S. 1993. Inhibition of tumor promoter-induced hydrogen peroxide formation in vitro and in vivo by genistein. Nutr. Cancer. 20(1): 1-12. https://doi.org/10.1080/01635589309514265