Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Phylogenetic Relationships of 3 Korean Neodiplostomum Species (Digenea: Neodiplostomidae) Based on Partial CO1 Gene

  • Pyo, Kyoung-Ho (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Lee, Jo Woon Yi (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Lee, Jin-Ju (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Park, Yun-Kyu (Department of Parasitology, Inha University College of Medicine) ;
  • Shin, Eun-Hee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine)
  • Received : 2014.04.26
  • Accepted : 2014.05.26
  • Published : 2014.06.30
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Abstract

The phylogenetic relationships of the 3 Neodiplostomum spp. (Digenea: Neodiplostomidae) occurring in Korea (N. seoulense, N. leei, and N. boryongense) were analyzed using the partial mitochondrial cytochrome c oxidase subunit 1 (CO1) gene. The adult flukes were recovered from Sprague-Dawley rats (N. seoulense) and newborn chicks (N. leei and N. boryongense) experimentally infected with the neodiplostomula from the grass snake, Rhabdophis tigrinus tigrinus. The genomic DNA was amplified using specific primers, and the sequence of CO1 was obtained. According to the results, the pairwise similarity was 96.1% between N. boryongense and N. seoulense, but was 95.0% between N. boryongense and N. leei and 94.2% between N. leei and N. seoulense. The results demonstrated a closer phylogenetic relationship between N. seoulense and N. boryongense. This high relationship of N. seoulense and N. boryongense may be related to their similar morphologic features including the limited distribution of vitellaria and the presence of a genital cone. N. leei is distinct on the other hand with an extensive distribution of vitellaria and the absence of a genital cone.

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References

  1. Chai JY, Sohn WM, Chung HL, Hong ST, Lee SH. Metacercariae of Pharyngostomum cordatum found from the European grass snake, Rhabdophis tigrina, and its experimental infection to cats. Korean J Parasitol 1990; 28: 175-181. https://doi.org/10.3347/kjp.1990.28.3.175
  2. Chai JY, Shin EH. Neodiplostomum leei n. sp. (Digenea: Neodiplostomidae) from chicks infected with metacercariae from the grass snake Rhabdophis tigrina. J Parasitol 2002; 88: 1181-1186. https://doi.org/10.1645/0022-3395(2002)088[1181:NLNSDN]2.0.CO;2
  3. Shin EH, Kim JL, Chai JY. A new neodiplostomid (Digenea) from the intestine of chicks infected with metacercariae from the grass snake, Rhabdophis tigrina. J Parasitol 2008; 94: 1379-1384. https://doi.org/10.1645/GE-1499.1
  4. Seo BS, Rim HJ, Lee CW. Studies on the parasitic helminthes of Korea I. Trematodes of rodents. Korean J Parasitol 1964; 2: 20-26. https://doi.org/10.3347/kjp.1964.2.1.20
  5. Dolezel D, Koudela B, Jirku M, Hypsa V, Obornik M, Votypka J, Modry D, Slapeta JR, Lukes J. Phylogenetic analysis of Sarcocystis spp. of mammals and reptiles supports the coevolution of Sarcocystis spp. with their final hosts. Int J Parasitol 1999; 29: 795-798. https://doi.org/10.1016/S0020-7519(99)00018-1
  6. Hebert PDN, Ratnasingham S, deWarrd JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc R Soc Lond B 2003; 270(Suppl): S96-S99. https://doi.org/10.1098/rsbl.2003.0025
  7. Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 1992; 54: 165-173. https://doi.org/10.1016/0166-6851(92)90109-W
  8. Varcasia A, Lightowlers MW, Cattoli G, Cancedda GM, Canu S, Garippa G, Scala A. Genetic variation within Taenia multiceps in Sardinia, western Mediterranean (Italy). Parasitol Res 2006; 99: 622-626. https://doi.org/10.1007/s00436-006-0179-y
  9. Okamoto M, Bessho Y, Kamiya M, Kurosawa T, Horii T. Phylogenetic relationships within Taenia taeniaeformis variants and other taeniid cestodes inferred from the nucleotide sequence of the cytochrome c oxidase subunit 1 gene. Parasitol Res 1995; 81: 451-458. https://doi.org/10.1007/BF00931785
  10. Morgan JAT, Blair D. Relative merits of nuclear ribosomal internal transcribed spacers and mitochondrial CO1 and ND1 genes for distinguishing among Echinostoma species (Trematoda). Parasitology 1998; 116: 289-297. https://doi.org/10.1017/S0031182097002217
  11. Lee SU, Huh S, Sohn WM. Molecular phylogenic location of the Plagiorchis muris (Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome c oxidase subunit 1. Korean J Parasitol 2004; 42: 71-75. https://doi.org/10.3347/kjp.2004.42.2.71
  12. Li T, Zhan B, Hawdon JM, Gong X, Xiao S, Shan Q, Feng Z, Hotez PJ. Sequencing of cytochrome c oxidase 1 gene of Ancylostoma duodenale and Necator americanus. Chinese J Parasitol Parasitic Dis 1999; 17: 81-83.
  13. Okamoto M, Iseto C, Shibahara T, Sato MO, Wandra T, Craig PS, Ito A. Intraspecific variation of Spirometra erinaceieuropaei and phylogenetic relationship between Spirometra and Diphyllobothrium inferred from mitochondrial CO1 gene sequences. Parasitol Int 2007; 56: 235-238. https://doi.org/10.1016/j.parint.2007.03.003
  14. Okamoto M, Urushima H, Iwasa M, Hasegawa H. Phylogenetic relationship of rodent pineworms (genus Syphacia) in Japan inferred from mitochondrial CO1 gene sequences. J Vet Med Sci 2007; 69: 545-547. https://doi.org/10.1292/jvms.69.545
  15. Rodrigues AC, Paiva F, Campaner M, Stevens JR, Noyes HA, Teixeira MMG. Phylogeny of Trypanosoma (Megatrypanum) theileri and related trypanosomes reveals lineages of isolates associated with artiodactyl hosts diverging on SSU and ITS ribosomal se-quences. Parasitology 2006; 132: 215-224.
  16. Crozier RH, Crozier YC, Mackinlay AG. The CO-I and CO-II region of honeybee mitochondrial DNA: evidence for variation in insect mitochondrial evolutionary rates. Mol Biol Evol 1989; 6: 399-411.
  17. Shin EH, Kim IM, Kim JL, Han ET, Park YK, Nawa Y, Kook J, Lee SH, Chai JY. Migration of Neodiplostomum leei (Digenea: Neodiplostomidae) neodiplostomula to the livers of various mammals. J Parasitol 2006; 92: 223-229. https://doi.org/10.1645/GE-649R.1
  18. Alizon S, Baalen M. Acute or chronic? within-host models with immune dynamics, infection outcome, and parasite evolution. Am Nat 2008; 172: E244-E256. https://doi.org/10.1086/592404

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