Parasites, Hosts and Diseases

  • 제52권3호
  • /
  • Pages.311-315
  • /
  • 2014
  • /
  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지
DOI QR코드

DOI QR Code

In Vitro Trypanocidal Activity of Macela (Achyrocline satureioides) Extracts against Trypanosoma evansi

  • Baldissera, Matheus D. (Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM)) ;
  • Oliveira, Camila B. (Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM)) ;
  • Zimmermann, Carine E.P. (Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM)) ;
  • Boligon, Aline A. (Research Laboratory of Phytochemistry, Department of Industrial Pharmacy, Universidade Federal de Santa Maria) ;
  • Athayde, Margareth Linde (Research Laboratory of Phytochemistry, Department of Industrial Pharmacy, Universidade Federal de Santa Maria) ;
  • Bolzan, Leandro P. (Laboratory of Microbiology, Ciencias da Saude, Centro Universitario Franciscano) ;
  • Vaucher, Rodrigo De A. (Laboratory of Microbiology, Ciencias da Saude, Centro Universitario Franciscano) ;
  • Santurio, Janio M. (Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM)) ;
  • Sagrillo, Michele R. (Cell Culture Laboratory, Centro Universitario Franciscano (UNIFRA)) ;
  • da Silva, Aleksandro Schafer (Department of Animal Science, Universidade do Estado de Santa Catarina) ;
  • Monteiro, Silvia G. (Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM))
  • 투고 : 2013.11.24
  • 심사 : 2014.04.11
  • 발행 : 2014.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The aim of this study was to verify the trypanocidal effectiveness of aqueous, methanolic, and ethanolic extracts of Achyrocline satureioides against Trypanosoma evansi in vitro. A. satureioides extracts, known as macela, were used on trypomastigotes at different concentrations (1, 5, 10, 50, 100, 500, and $1,000{\mu}g/ml$) and exposure times (0, 1, 3, 6, and 9 hr). A dose-dependent effect was observed when the 3 extracts were tested. The concentrations of 1, 5, and $10{\mu}g/ml$ were not able to kill trypomastigotes until 3 hr after exposure, and the highest concentrations (500 and $1,000{\mu}g/ml$) were able to kill all trypomastigotes after 1 hr. When the time of exposure was increased up to 9 hr, the concentrations at 50 and $100{\mu}g/ml$ were 100% effective to 3 extracts. The chemical analysis of the extracts revealed the presence of flavonoids, a trypanocidal compound already described. Based on the results, we can conclude that the A. satureioides extracts exhibit trypanocidal effects.

키워드

참고문헌

  1. Hnatyszyn O, Moscatelli V, Rondina R, Costa M, Arranz C, Balaszczuk A, Coussio JD, Ferraro GD. Flavonoids from Achyrocline satureioides with relaxant effects on the smooth muscle of guinea pig corpus cavernosum. Phytomedicine 2004; 11: 366-369. https://doi.org/10.1078/0944711041495182
  2. Silva LFD, Langeloh A. Comparative study of antispasmodic activity of hidroalcoholic 80% (v/v) of Achyrocline satureioides (Lam) DC (Asteraceae) with papaverine and atropine on rat isolated jejunum. Acta Farm Bonaer 1994; 13: 35-40.
  3. Garcia G, Campos R, De Torres R, Broussalis A, Ferraro G, Martino V, Coussio JD. Antiherpetic activity of some Argentine medicinal plants. Fitoterapia 1990; 61: 542-546.
  4. Gillij YJ, Gleiser RM, Zygadlo JA. Mosquito repellent activity of essential oils of aromatic plants growing in Argentina. Bioresource Technol 2008; 99: 2507-2515. https://doi.org/10.1016/j.biortech.2007.04.066
  5. Ferraro G, Anesini C, Ouvina A. Total phenolic content and antioxidant activity of extracts of Achyrocline satureioides flowers from different zones in Argentina. Lat Am J Pharm 2008; 27: 626-628.
  6. Carini JP, Klamt F, Bassani VL. Flavonoids from Achyrocline satureioides: Promising biomolecules for anticancer therapy. RSC Advances 2014; 4: 3131-3144. https://doi.org/10.1039/C3RA43627F
  7. Brandelli CLC, Giordani RB, De Carli GA, Tasca T. Indigenous traditional medicine: in vitro anti-giardial activity of plants used in the treatment of diarrea. Parasitol Res 2009; 104: 1345-1349. https://doi.org/10.1007/s00436-009-1330-3
  8. Macedo M, Consoli R, Grandi T, Dos Anjos A, De Oliveira A, Mendes N, Queiroz R, Zani C. Screening of Asteraceae (Compositae) plant extracts for larvicidal activity against Aedes fluviatilis (Diptera: Culicidae). Mem Inst Oswaldo Cruz 1997; 92: 565-570. https://doi.org/10.1590/S0074-02761997000400024
  9. Silva RAMS, Seidl A, Ramirez L, Davila AMR. Trypanosoma evansi e Trypanosoma vivax: Biologia, Diagnostico e Controle. Corumba, Embrapa Pantanal. 2002, p 1-162.
  10. Brun R, Hecker H, Lun Z. Trypanosoma evansi and Trypanosoma equiperdum: distribution, biology, treatment and phylogenetic relationship (a review). Vet Parasitol 1998; 79: 95-107. https://doi.org/10.1016/S0304-4017(98)00146-0
  11. Maudlin I, Holmes PH, Miles MA. The Trypanosomiases. Wallingford, UK. CABI Publishing Co. 2004, p 640.
  12. Spinosa HS, Gorniak SL, Bernardi MM. Farmacologia aplicada a medicina veterinaria. Rio de Janeiro, Guanabara koogan. 1999, p 450.
  13. Kaminsky R, Brun R. In vivo and In vitro activities of trybizine hydrochloride against various pathogenic trypanosome species. Antimicrob Agents Chemother 1998; 42: 2858-2863.
  14. Simões CMO, Schenkel EP., Gosmann G, Mello JCP, Mentz LA, Petrovick PR. Farmacognosia da planta ao medicamento. Porto Alegre, Editora UFRGS. 2004, p 1102.
  15. Boligon AA, Sagrillo MR, Machado LF, Filho OS, Machado MM, Da Cruz IBM, Athayde ML. Protective effects of extracts and flavonoids isolated from Scutia buxifolia Reissek against chromosome damage in human lymphocytes exposed to hydrogen peroxide. Molecules 2012; 17: 5757-5769. https://doi.org/10.3390/molecules17055757
  16. Boligon AA, Kubica TK, Mario DB, Brum TF, Piana M, Weiblen R, Lovato L, Alves SH, Santos RCV, Alves CFS, Athayde ML. Antimicrobial and antiviral activity-guided fractionation from Scutia buxifolia Reissek extracts. Acta Physiol Plant 2013; 35: 2229-2239. https://doi.org/10.1007/s11738-013-1259-0
  17. Baldissera M.D, Da Silva AS, Oliveira CB, Zimmermann CEP, Vaucher RA, Santos RCV, Rech VC, Tonin AA, Giongo JL, Mattos CB, Koester L, Santurio JM, Monteiro SG. Trypanocidal activity of the essential oils in their conventional and nanoemulsion forms: in vitro tests. Exp Parasitol 2013; 134: 356-361. https://doi.org/10.1016/j.exppara.2013.03.035
  18. Gillingwater K, Kumar A, Anbazhagan M, Boykin DW, Tidwell RR, Brun R. In vivo investigations of selected diamidine coumpounds against Trypanosoma evansi using a mouse model. Antimicrob Agents Chemother 2009; 53: 5074-5079. https://doi.org/10.1128/AAC.00422-09
  19. Baltz T, Baltz D, Giroud C, Crockett J. Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense. EMBO J 1985; 4: 1273-1277.
  20. Retta D, Dellacassa E, Villamil J, Suarez AS, Bandoni AL. Marcela, a promising medicinal and aromatic plant from Latin America: a review. Ind Crop Prod 2012; 38: 27-38. https://doi.org/10.1016/j.indcrop.2012.01.006
  21. Trojan - Rodrigues M, Alves TLS, Soares GLC, Ritter MR. Plant used as antidiabetics in popular medicine in Rio Grande do Sul, southern Brazil. J Ethnopharmacol 2012; 139: 155-163. https://doi.org/10.1016/j.jep.2011.10.034
  22. Scheidewind EM, Buge A, Kala H, Metzner J, Zschunke A. Identification of a antimicrobial constituent isolated from propolis. Pharmazie 1979; 34: 103-106.
  23. Grael CF, Albuquerque S, Lopes JL. Chemical constituents of Lichnophora pohlii and trypanocidal activity of crude plant extracts and of isolated compounds. Fitoterapia 2005; 76: 73-82. https://doi.org/10.1016/j.fitote.2004.10.013
  24. Tasdemir D, Kaiser M, Brun R, Yardley V, Schmidt TJ, Tosun F, Ruedi P. Antitrypanosomal and antileishmanial activities of flavonoids and their analogues: in vitro, in vivo, structure-activity relationship, and quantitative structure-activity relationship studies. Antimicrob Agents Chemother 2006; 50: 1352-1364. https://doi.org/10.1128/AAC.50.4.1352-1364.2006
  25. Mamadalieva NF, Hermann F, El-Readi MZ, Tahrani A, Hamound R, Egamberdieva DR, Azimova SS, Wink M. Flavonoids in Scutellaria immaculata and S. ramosissima (Lamiaceae) and their biological activity. J Pharm Pharmacol 2011; 63: 1346-1357. https://doi.org/10.1111/j.2042-7158.2011.01336.x
  26. Izumi E, Ueda - Kakamura T, Veiga Junior VF, Pinto AC, Nakamura CV. Terpenes from Copaifera demonstrated in vitro antiparasitic and synergic activity. J Med Chem 2012; 55: 2994-3001. https://doi.org/10.1021/jm201451h
  27. Mittra B, Saha A, Chowdhury AR, Pal C, Mandal S, Mukhopadhyay S, Bandyopadhyay S, Majumder HK. Luteolin, an abundant dietary component is a potent anti-leishmanial agent that acts by inducing topoisomerase II-mediated kinetoplast DNA cleavage leading to apoptosis. Mol Med 2006; 6: 527-541.

피인용 문헌

  1. Untargeted LC-MS metabolomic studies of Asteraceae species to discover inhibitors of Leishmania major dihydroorotate dehydrogenase vol.15, pp.4, 2014, https://doi.org/10.1007/s11306-019-1520-7
  2. Trypanocidal Essential Oils: A Review vol.25, pp.19, 2014, https://doi.org/10.3390/molecules25194568