The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of the 70% ethanol extract of Mori Cortex Radidus on ovalbumin-induced allergic asthma in mice

난알부민으로 유도된 천식 마우스에서 상백피 추출물의 면역조절효능 연구

  • Kang, Seok Yong (Department of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Woo, Eun-Rhan (College of Pharmacy, Chosun University) ;
  • Park, Yong-Ki (Department of Herbology, College of Oriental Medicine, Dongguk University)
  • 강석용 (동국대학교 한의과대학 본초학교실) ;
  • 우은란 (조선대학교 약학대학) ;
  • 박용기 (동국대학교 한의과대학 본초학교실)
  • Received : 2014.06.17
  • Accepted : 2014.07.22
  • Published : 2014.07.30
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Abstract

Objectives : The root bark of Morus alba L. (Mori Cortex Radidus; MCR) has been traditionally used to reduce heat from the lungs, soothe asthma, and edema and to promote urination. In this study, we investigated the effect of MCR ethanol extract on ovalbumin (OVA)-induced allergic asthma in mice. Methods : Mice were sensitized at day 0, 7 and 14 with 0.2% OVA and then airway challenged at day 21, 23, 25, 27 and 29 to induce allergic asthma. MCR extracts at doses of 50 and 100 mg/kg body weight (bw) were orally administered during OVA challenge once per a day. The levels of allergic mediators such as histamine, OVA-specific IgE, IFN-${\gamma}$ and IL-4 were measured in the sera of mice by ELISA. The histopathological change of lung tissues was observed with hematoxylin and eosin (H&E) staining. Results : MCR extract significantly decreased not only the serum levels of histamine, OVA-specific IgE, and IL-4 compared with those of OVA control group, but significantly increased the serum level of IFN-${\gamma}$. In H&E staining, MCR extract inhibited the infiltration of inflammatory cells and bronchiolar damage with epithelial thickening in lung tissues of OVA-induced asthma mice. Conclusions : These results indicate that MCR extract inhibits lung damage by asthma through regulating the allergic immune response, suggesting that MCR may be used as a useful agent for the treatment of allergic asthma.

Keywords

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