Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Anti-Inflammatory Effect of Mangostenone F in Lipopolysaccharide-Stimulated RAW264.7 Macrophages by Suppressing NF-κB and MAPK Activation

  • Cho, Byoung Ok (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Ryu, Hyung Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • So, Yangkang (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Chang Wook (Department of Food Science and Human Nutrition, Chungnam National University) ;
  • Jin, Chang Hyun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Yook, Hong Sun (Department of Food Science and Human Nutrition, Chungnam National University) ;
  • Jeong, Yong Wook (Department of Microbiology, College of Medicine, Seonam University) ;
  • Park, Jong Chun (Department of Microbiology, College of Medicine, Seonam University) ;
  • Jeong, Il Yun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • Received : 2014.05.08
  • Accepted : 2014.06.10
  • Published : 2014.07.31
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Abstract

Mangostenone F (MF) is a natural xanthone isolated from Garcinia mangostana. However, little is known about the biological activities of MF. This study was designed to investigate the anti-inflammatory effect and underlying molecular mechanisms of MF in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. MF dose-dependently inhibited the production of NO, iNOS, and pro-inflammatory cytokines (TNF-${\alpha}$, IL-6, and IL-$1{\beta}$) in LPS-stimulated RAW264.7 macrophages. Moreover, MF decreased the NF-${\kappa}B$ luciferase activity and NF-${\kappa}B$ DNA binding capacity in LPS-stimulated RAW264.7 macrophages. Furthermore, MF suppressed the NF-${\kappa}B$ activation by inhibiting the degradation of $I{\kappa}B{\alpha}$ and nuclear translocation of p65 subunit of NF-${\kappa}B$. In addition, MF attenuated the AP-1 luciferase activity and phosphorylation of ERK, JNK, and p38 MAP kinases. Taken together, these results suggest that the anti-inflammatory effect of MF is associated with the suppression of NO production and iNOS expression through the down-regulation of NF-${\kappa}B$ activation and MAPK signaling pathway in LPS-stimulated RAW264.7 macrophages.

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References

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