생체재료학회지 (Biomaterials Research)

  • 제18권1호
  • /
  • Pages.8-17
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  • 2014
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  • 1226-4601(pISSN)
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  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지

The Identification of a Receptor-binding Peptide Derived from Bone Morphogenetic Protein-6 and Its Role in Osteogenesis

  • Choi, Yoon Jung (Dental Regenerative Biotechnology major, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Lee, Jue Yeon (Research Center, Nano Intelligent Biomedical Engineering Corporation (NIBEC)) ;
  • Chung, Chong Pyoung (Research Center, Nano Intelligent Biomedical Engineering Corporation (NIBEC)) ;
  • Park, Yoon Jeong (Dental Regenerative Biotechnology major, School of Dentistry and Dental Research Institute, Seoul National University)
  • 투고 : 2013.08.20
  • 심사 : 2013.10.15
  • 발행 : 2014.03.01
⟨ 이전 논문 다음 논문 ⟩

초록

Bone morphogenetic protein (BMP)-6 induces the differentiation of mesenchymal-derived cells into osteochondrogenic cells. Similar to the potent osteochondrogenic growth factor BMP-2, the BMP-6 also contains the "knuckle epitope", which is thought to bind BMP receptor type II. In this study, we analyzed a synthetic peptide, YVPLPCCAPTKLNAISVLYF, namely BMP receptor binding peptide (BRBP), corresponding to residues of XX-YZ of the knuckle epitope of BMP-6. Our data revealed that the BRBP significantly binds to both type I and type II BMP receptors (BMPRs) with a similar affinity to BMP-6. The BRBP also induces osteogenic differentiation of human mesenchymal stem cells (HMSCs) with similar potency to BMP-6 as measured by alkaline phosphatase (ALP) activity, mineralization and osteogenic gene marker expression. Furthermore, the BRBP stimulates the phosphorylation of both the extracellular signal-regulated kinase (ERK1/2), and the Smad1/5/8 proteins. Blocking ERK-signaling with U0126, an inhibitor for MAPK/ERK kinase (MEK), abolishes the oseteogenic effects of the BRBP on human mesenchymal stem cells (HMSCs). Thus our study identifies an osteogenic peptide derived from BMP-6, which may be useful for ossified tissue regeneration.

키워드

참고문헌

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