Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Prevalence of Methicillin-resistant Staphylococci Isolates from Horses and Horse-related Personnel in Korea

말 및 말관련 종사자의 methicillin 내성 포도상구균의 유병율 조사

  • Lee, Sang-Kyu (Equine Hospital, Korea Racing Authority) ;
  • Han, Jae-Ik (College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Ill-Hwa (College of Veterinary Medicine, Chungbuk National University) ;
  • Na, Ki-Jeong (College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Hyun-Gu (College of Veterinary Medicine, Chungbuk National University)
  • Accepted : 2014.04.29
  • Published : 2014.06.30
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Abstract

Methicillin-resistant staphylococci (MRS) are emerging as important pathogens in humans and animals worldwide. The aim of this study was to investigate the prevalence of MRS in the racehorse population and in horse-related personnel in Korea. A total of 195 horses and 18 humans (eight veterinarians, three veterinary hospital staff, and seven horse-handlers) from racehorse farms in Korea were included in the study. The samples were collected from nasal cavities using bacterial transport medium and were cultivated on tryptic soy agar with 5% sheep blood for 3 days at $37^{\circ}C$ to confirm the presence of Staphylococcus spp. Presumptive Staphylococcus spp. isolates were identified by 16S ribosomal RNA gene analysis. The coagulase test and oxacillin susceptibility tests were performed using the tube dilution and disk diffusion methods, respectively. The presence of the mecA gene was determined using a polymerase chain reaction assay. Of the 195 horses, 29 (15.6%) yielded 29 MRS isolates. Twelve (66.7%) of the 18 horse-related personnel yielded 12 MRS isolates. All of the MRS isolates from horses or horse-related personnel were identified as methicillin-resistant coagulase-negative staphylococci (MRCNS). The result of this study suggest that the prevalence of MRS increased with the duration of antibiotic use (p = 0.002). This study also provides evidence for the zoonotic transmission of MRCNS between horses and humans, although further investigations are needed.

Methicillin 내성 포도상구균은 전세계적으로 사람과 동물에서 중요한 병인체로 주목 받고 있다. 본 연구는 국내 말과 말을 취급하는 사람에서의 methicillin 내성 포도상구균 발생현황을 조사하고자 실시하였다. 국내 경주마 목장에 소재하는 총 195두의 말과 18명의 말을 취급하는 사람(8명의 수의사, 7명의 말 관리사, 3명의 동물병원 직원)을 대상으로 하였다. 면봉을 이용하여 한쪽 비강에서 시료를 채취하여 세균수송배지에 보관 후 5% 양 혈액배지에서 $37^{\circ}C$ 3일간 배양하여 포도상구균 존재여부를 확인하였다. 포도상구균은 16S rRNA 유전자 분석을 실시하여 동정하였으며, 동정된 포도상구균은 coagulase 검사를 실시하였다. Methicillin 저항성을 확인하기 위하여 oxacillin 디스크 검사와 함께 mecA 유전자 존재를 PCR을 통하여 확인하였다. 검사를 실시하였던 말 195두 중 64두가 포도상구균으로 동정되었으며, 이중 29두(44.6%)가 methicillin 내성 포도상구균으로 확인되었다. 말을 취급하는 18명 중 14명의 시료에서 포도상구균이 동정되었으며, 이중 12명(85.7%)의 시료에서 methicillin에 내성을 가지고 있는 포도상구균으로 확인되었다. 말과 사람에서 동정된 모든 methicillin 내성 포도상구균은 coagulase 음성으로 확인되었다. 또한 항생제의 사용기간이 긴 개체에서 사용기간이 짧았던 개체군보다 methicillin 내성 포도상구균이 높은 것으로 나타났다(p = 0.002). 본 연구결과는 사람과 말 사이에서 인수공통전파가 일어날 수 있음을 시사한다.

Keywords

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