DOI QR코드

DOI QR Code

Expression of Neurotrophic Factors and Their Receptors in Rat Posterior Taste Bud Cells

  • Park, Dong-Il (Department of Physiology and Neuroscience, College of Dentistry, Gangneung-Wonju National University) ;
  • Chung, Ki-Myung (Department of Physiology and Neuroscience, College of Dentistry, Gangneung-Wonju National University) ;
  • Cho, Young-Kyung (Department of Physiology and Neuroscience, College of Dentistry, Gangneung-Wonju National University) ;
  • Kim, Kyung-Nyun (Department of Physiology and Neuroscience, College of Dentistry, Gangneung-Wonju National University)
  • Received : 2014.04.09
  • Accepted : 2014.06.03
  • Published : 2014.06.30

Abstract

Taste is an important sense in survival and growth of animals. The growth and maintenance of taste buds, the receptor organs of taste sense, are under the regulation of various neurotrophic factors. But the distribution aspect of neurotrophic factors and their receptors in distinct taste cell types are not clearly known. The present research was designed to characterize mRNA expression pattern of neurotrophic factors and their receptors in distinct type of taste cells. In male 45-60 day-old Sprague-Dawley rats, epithelial tissues with and without circumvallate and folliate papillaes were dissected and homogenized, and mRNA expressions for neurotrophic factors and their receptors were determined by RT-PCR. The mRNA expressions of brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3), receptor tyrosine kinase B (TrkB), exclusion of nerve growth factor (NGF), neurotrophin-4/5 (NT4/5), receptor tyrosine kinase A (TrkA), receptor tyrosine kinase C (TrkC), and p75NGFR were observed in some population of taste cell. In support of this result and to characterize which types of taste cells express NT3, BDNF, or TrkB, we examined mRNA expressions of NT3, BDNF, or TrkB in the $PLC{\beta}2$ (a marker of Type II cell)-and/or SNAP25 (a marker of Type III cell)-positive taste cells by a single taste cell RT-PCR and found that the ratio of positively stained cell numbers were 17.4, 6.5, 84.1, 70.3, and 1.4 % for $PLC{\beta}2$, SNAP25, NT3, BDNF, and TrkB, respectively. In addition, all of $PLC{\beta}2$-and SNAP25-positive taste cells expressed NT3 mRNA, except for one taste bud cell. The ratios of NT3 mRNA expressions were 100% and 91.7% in the SNAP25-and $PLC{\beta}2$-positive taste cells, respectively. However, two TrkB-positive taste cells co-expressed neither $PLC{\beta}2$ nor SNAP 25. The results suggest that the most of type II or type III cells express BDNF and NT3 mRNA, but the expression is shown to be less in type I taste cells.

Keywords

References

  1. Kim KN, Chun SW. Special Senses. in Physiology in Denistry 2nd ed. edited by Kim JS et al. pp 497-509, DaehanNarae Publishing Co. Seoul, 2009
  2. Murray RG, Murray A, Fujimito S. Fine structure of gustatory cells in rabbit taste buds. J Ultrastr Res. 1969;27:444-461. https://doi.org/10.1016/S0022-5320(69)80043-2
  3. Fujimoto S, Murray RG. Fine structure of degeneration and regeneration in denervated rabbit vallate taste buds. Anat Rec. 1970;168:393-414. https://doi.org/10.1002/ar.1091680306
  4. Pumplin DW, Getschman E, Boughter JD Jr, Yu C, Smith DV. Differential expression of carbohydrate blood-group antigens on rat taste bud cells:relation to the functional marker alpha-gustducin. J Comp Neurol. 1999;415: 230-239. https://doi.org/10.1002/(SICI)1096-9861(19991213)415:2<230::AID-CNE7>3.0.CO;2-Y
  5. Clapp TR, Stone LM, Margolskee RF, Kinnamon SC. Imunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction. BMC Neurosci. 2001;2:6. https://doi.org/10.1186/1471-2202-2-6
  6. Kim DJ, Roper SD. Localization of serotonin in taste buds:a comparative study in four vertebrates. J Comp Neurol. 1995;353:364-370. https://doi.org/10.1002/cne.903530304
  7. Yee CL, Yang R, Finger TE, Kinnamon JC. "Type III" cells of rat taste buds: immunohistochemical and ultrastructural syudies of neuron-specific enolase, protein gene product 9.5 and serotonin. J Comp Neurol. 2001;440:97-108. https://doi.org/10.1002/cne.1372
  8. Takeda M, Suzuki Y, Obara N, Nagai Y. Neural cell adhesion molecule of taste buds. J Electron Microsc. 1992;41:375-380.
  9. Oakley B, Witt M. Building sensory receptors on the tongue. J Neurol. 2005;33:631-646
  10. Fritzsch B, Sarai PA, Barbacid M, Silos-Santiago I. Mice with a targeted disruption of the neurotrophin receptor trkB lose their gustatory ganglion cells early but do develop taste buds. Int J Dev Neurosci 1997;15:563-576 https://doi.org/10.1016/S0736-5748(96)00111-6
  11. Cooper D, Oakley B. Fuctional redundancy and gustatory development in BDNF null mutant mice. Dev Brain Res. 1998;105:79-84. https://doi.org/10.1016/S0165-3806(97)00167-3
  12. Kim KN, Caicedo A, and Roper SD. Glutamate-induced cobalt uptake reveals non-NMDA receptors in developing rat taste buds. NeuroReport 2001;12:1715-1718. https://doi.org/10.1097/00001756-200106130-00039
  13. Patterson SL, Schwartzkroin PA, Bothwell M. Neurotrophin expression in rat hippocampal slices: a stimulus paradigm including LTP in CA1 evokes increases in BDNF and NT-3 mRNAs. Neuron 1992;9:1081-1088 https://doi.org/10.1016/0896-6273(92)90067-N
  14. Masako T, Yuko S, Nobuko O, Hiroaki T. Immunihistochemical detection of neurotrophin-3 and -4 and their receptors in mouse taste bud cells. Arch Histol Cytol. 2005;68:393-403 https://doi.org/10.1679/aohc.68.393
  15. Takeda M, Suzuki Y, Obara N, Ucida N, Kawakoshi K. Expression of GDNF and GFR${\alpha}$1 in mouce taste bud cells. J Comp Neurol. 2004;479:94-102. https://doi.org/10.1002/cne.20315
  16. Ganchrow D, Ganchrow JR, Verdin-Alcazar M, Whitehead MC. Brain-derived neurotrophic factor-, neurotrophin-3-, and tyrosine kinase receptor-like immunoreactivity in lingual taste bud fields of mature hamster. J Comp Neurol. 2003;455:11-24. https://doi.org/10.1002/cne.2162
  17. Yee CL, Jones KR, Finger TE. Brain-derived neurotrophic factor is present in adult mouse taste cells with synapses. J Comp Neurol. 2003;459:15-24. https://doi.org/10.1002/cne.10589
  18. Nostrat CA, Ebendal T, Olson L. Differential expression of brain-derived neurotrophic factor and neurotrophin 3 mRNA in lingual papillae and taste buds indicates roles in gustatory and somatosensory innervation. J Comp Neurol. 1996;376:587-602. https://doi.org/10.1002/(SICI)1096-9861(19961223)376:4<587::AID-CNE7>3.0.CO;2-Y
  19. Finger TE, Danilova V, Barrows J, Bartel DL, Vigers AJ, Stone L, Hellekant G, Kinnamon SC. ATP signaling is crucial for communication from taste buds to gustatory nerves. Science 2005;10:1495-1499.
  20. Huang YA, Pereira E, Roper SD. Acid stimulation (sour taste) elicits GABA and serotonin release from mouse taste cells. PLoS ONE 2011;6:e25471 https://doi.org/10.1371/journal.pone.0025471
  21. Dvoryanchikov G, Huang YA, Barro-Soria R, Chaudhari N, Roper SD. GABA, its receptors, and GABAergic inhibition in mouse taste buds. J Neurosci. 2011; 31:5782-5791. https://doi.org/10.1523/JNEUROSCI.5559-10.2011
  22. Ito A, Nosrat IV, Nosrat CA. Taste cell formation does not require gustatory and somatosensory innervation. Neurosci Lett. 2010;471:189-194. https://doi.org/10.1016/j.neulet.2010.01.039
  23. Nosrat IV, Agerman K, Marinescu A, Ernfors P, Nosrat CA. Lingual deficits in neurotrophin double knockout mice. J Neurocytol. 2004;33:607-615 https://doi.org/10.1007/s11068-005-3330-2
  24. Lindemann B. Taste reception. Physiol Rev. 1996;76:719-766. https://doi.org/10.1152/physrev.1996.76.3.719
  25. DeFazio RA, Dvoryanchikov G,. Maruyama Y, Kim JW, Pereira E, Roper SD, and Chaudhari N. Seperate populations of receptor cells and presynaptic cells in mouse taste buds. J Neurosci. 2006;26:3971-3980. https://doi.org/10.1523/JNEUROSCI.0515-06.2006
  26. Lee SB, Lee CH, Cho YK, Chung KM, Kim KN. Expression of Kainate Glutamate Receptors in Type II Cells in Taste Buds of Rats. Int J Oral Biol. 2008;33:83-99.
  27. Laudes T, Meis S, Munsch T, Lessmann V. Impaired transmission at corticothalamic excitatory inputs and intrathalamic GABAergic synapses in the ventrobasal thalamus of heterozygous BDNF knockout mice. Neuroscience 2012;222:215-227 https://doi.org/10.1016/j.neuroscience.2012.07.005