Plant Breeding and Biotechnology

  • 제2권3호
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  • Pages.224-230
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  • 2014
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  • 2287-9358(pISSN)
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  • 2287-9366(eISSN)
한국육종학회 (The Korean Society of Breeding Science)
권호 표지
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Development of SCAR Markers for Korean Wheat Cultivars Identification

  • Son, Jae-Han (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA) ;
  • Kim, Kyeong-Hoon (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA) ;
  • Shin, Sanghyun (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA) ;
  • Choi, Induk (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA) ;
  • Kim, Hag-Sin (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA) ;
  • Cheong, Young-Keun (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA) ;
  • Lee, Choon-Ki (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA) ;
  • Lee, Sung-Il (Department of Molecular Bioscience, Kangwon National University) ;
  • Choi, Ji-Yeong (Department of Molecular Bioscience, Kangwon National University) ;
  • Park, Kwang-Geun (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA) ;
  • Kang, Chon-Sik (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA)
  • 투고 : 2014.07.25
  • 심사 : 2014.08.24
  • 발행 : 2014.09.01
⟨ 이전 논문 다음 논문 ⟩

초록

Amplified fragment length polymorphism (AFLP) is a molecular marker technique based on DNA and is extremely useful in detection of high polymorphism between closely related genotypes like Korean wheat cultivars. Six sequence characterized amplified regions (SCARs) have been developed from inter simple sequence repeat (ISSR) analysis which enabled the identification and differentiation of 13 Korean wheat cultivars from the other cultivars. We used six combinations of primer sets in our AFLP analysis for developing additional cultivar-specific markers in Korean wheat. Fifty-eight of the AFLP bands were isolated from EA-ACG/MA-CAC, EA-AGC/MA-CTG and EA-AGG/MA-CTA primer combinations. Of which 40 bands were selected to design SCAR primer pairs for Korean wheat cultivar identification. Three of 58 amplified primer pairs, KWSM006, KWSM007 and JkSP, enabled wheat cultivar identification. Consequently, 23 of 32 Korean wheat cultivars were classified by eight SCAR marker sets.

키워드

과제정보

This study was supported by 2014 Postdoctoral Fellowship Program (PJ00875601) of National Institute of Crop Science, Rural Development Administration, Republic of Korea.