한국산학기술학회논문지 (Journal of the Korea Academia-Industrial cooperation Society)

  • 제15권6호
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  • Pages.3799-3804
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  • 2014
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  • 1975-4701(pISSN)
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  • 2288-4688(eISSN)
한국산학기술학회 (The Korea Academia-Industrial cooperation Society)
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형질전환 미세조류의 고주파 처리 배양을 통한 MAA 생산량 증가

Production Yield Enhancement of Mycosporine-like amino acid(MAA)s in Transformed Microalgae Culture by Radiofrequency

  • 서효현 (바이오에프디엔씨 안티에이징연구소) ;
  • 송미영 (바이오에프디엔씨 안티에이징연구소) ;
  • 아툴 쿨카르니 (바이오에프디엔씨 안티에이징연구소) ;
  • 서승석 (한국해양과학기술원 남해특성연구부) ;
  • 이택견 (한국해양과학기술원 남해특성연구부) ;
  • 모상현 (바이오에프디엔씨 안티에이징연구소)
  • Seo, Hyo Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Song, Mi Young (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Kulkarni, Atul (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Suh, Sung-Suk (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology) ;
  • Lee, Taek-Kyun (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology) ;
  • Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.)
  • 투고 : 2014.04.28
  • 심사 : 2014.06.12
  • 발행 : 2014.06.30
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초록

Mycosporine-like 아미노산(MAAs)은 UV 흡수물질이며, 다양한 해양생물들은 MAAs의 합성과 축적을 통하여 환경자외선의 직 간접적인 영향을 감소시키는 기능을 진화시켜 왔다. 이 연구에서는 미세조류, Chlamydomonas hedleyi에 포도당 전달 단백질인 Glucose transporter 1(Glut-1) 유전자를 pCAM1303 벡터에 도입한 형질전환체를 제작하여, 형질전환체의 바이오매스를 최대로 증가시킬 수 있는 최적의 Glucose 농도와 NH4Cl농도를 결정하고, 고주파(Radiofrequency) 발생장치를 활용한 바이오매스 증가와 함께 MAA를 대량 생산할 수 있는 배양 조건을 확립하였다. 연구결과 고주파 처리를 통한 형질전환 미세조류는 4.13 mg/L(MAAs/DCW)으로 3.23 mg/L(MAAs/DCW)의 고주파 처리 없이 배양한 형질전환체보다 효율이 증가하였다. 이러한 결과는 자외선 A 흡수물질을 인위적으로 증폭시킬 수 있어서, 대량배양한 후 MAAs물질을 분리 및 정제하여 피부자극성이 없는 친환경적인 자외선 차단 화장품 산업화에 크게 기여할 수 있음을 의미한다.

In sea water, microalgae are exposed to a range of critical environmental conditions. Microalgae are protected from UV-A radiation due to the presence of mycosporine like amino acids(MAAs). Owing to the UV-A absorption properties of MAAs, they are used widely as a UV protecting ingredient in cosmetics. Therefore, there is a need to increase the production yield of MAAs. This study investigated the production yield of MAAs in transformed microalgae by radiofrequency(RF) exposure. Initially, the Glut-1 gene was transformed to Chlamydomonas hedleyi microalgae as a glucose transporter. The biomass was enhanced after Glut-1 gene transformation. In addition, the MAAs production yield was increased during large scale production in bioreactors due to the RF treatment. Therefore, purified extracts of MAAs can be used as a sun block material in the cosmetic industrial field.

키워드

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