The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Characteristics of Two Unrecorded Yeasts from Wild Flowers in Ulleungdo, Korea

  • Hyun, Se-Hee (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Min, Jin-Hong (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Hyang Burm (Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Kim, Ha-Kun (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • Received : 2014.06.09
  • Accepted : 2014.06.17
  • Published : 2014.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Two unrecorded yeasts, Meyerozyma caribbica UL5-1 and Pichia silvicola UL6-1 were screened from 58 yeasts which were isolated from wild flowers in Ulleungdo in Gyeongsangbuk-do, Korea. The morphological and cultural characteristics of these unrecorded yeasts were investigated. Both yeasts were oval in shape and formed pseudomycelia. P. silvicola UL6-1 formed ascospore, but M. UL5-1 did not. P. silvicola UL6-1 and M. caribbica UL5-1 also grew in vitamin-free medium and 5% NaCl-containing yeast extract-peptone-dextrose medium. The two unrecorded yeasts assimilated glucose, galactose, xylose, cellobiose, trehalose, glycerol and sorbitol, and also fermented glucose, fructose and mannose. The supernatant of both M. caribbica UL5-1 and P. silvicola UL6-1 showed high antihypertensive angiotensin I-converting enzyme inhibitory activity of 84.2% and 82.6%, respectively. Cell-free extract of P. silvicola UL6-1 also showed very high anti-diabetic ${\alpha}$-glucosidase inhibitory activity (85.8%).

Keywords

References

  1. Jang IT, Kang MG, Na KC, Lee JS. Growth characteristics and physiological functionality of yeasts in pear marc extracts. Kor J Mycol 2011;39:170-3. https://doi.org/10.5941/MYCO.2011.39.3.170
  2. Jeong SC, Lee DH, Lee JS. Production and characterization of an anti-angiogenic agent from Saccharomyces cerevisiae K-7. J Microbiol Biotechnol 2006;16:1904-11.
  3. Kim YH. Production and in-vivo anti-diabetic activity of ${\alpha}$-glucosidase inhibitor from Pichia burtonii Y257-7 [dissertation]. Daejeon (Korea): University of Paichai; 2013.
  4. Lee JS, Yi SH, Kown SJ, Ahn C, Yoo JY. Enzyme activities and physiological functionality of yeasts from traditional Meju. Kor J Appl Microbiol Biotechnol 1997;25:448-53.
  5. Kim JH, Lee DH, Jeong SC, Chung KS, Lee JS. Characterization of antihypertensive angiotensin I-converting enzyme inhibitor from Saccharomyces cerevisiae. J Microbiol Biotechnol 2004;14:1318-23.
  6. Kim JH, Kim NM, Lee JS. Physiological characteristics and ethanol fermentation of thermotolerant yeast Saccharomyces cerevisiae OE-16 from traditional meju. Kor J Food Nutr 1999; 12:490-5.
  7. Kang MG, Hyun SH, Ryu JJ, Min JH, Kim HK, Lee JS. Note on newly isolated yeasts from wild flowers in Daejeon city, Korea. Kor J Mycol 2012;40:174-6. https://doi.org/10.4489/KJM.2012.40.3.174
  8. Min JH, Hyun SH, Kang MG, Lee HB, Kim CM, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers of Daejeon city and Chungcheongnam-do in Korea. Kor J Mycol 2012;40:141-4. https://doi.org/10.4489/KJM.2012.40.3.141
  9. Min JH, Ryu JJ, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers in Gyejoksan, Oseosan and Beakamsan of Korea. Kor J Mycol 2013;41:47-51. https://doi.org/10.4489/KJM.2013.41.1.47
  10. Hyun SH, Lee HB, Kim CM, Lee JS. New records of yeasts from wild flowers in coast near areas and inland areas, Korea. Kor J Mycol 2013;41:74-80. https://doi.org/10.4489/KJM.2013.41.2.74
  11. Min JH, Lee HB, Lee JS, Kim HK. Identification of yeasts isolated from wild flowers collected in coast areas of Korea based on the 26S rDNA sequences. Kor J Mycol 2013;41:185-91. https://doi.org/10.4489/KJM.2013.41.3.185
  12. Hyun SH, Mun HY, Lee HB, Kim HK, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do and Jeju island of Korea and production of anti-gout xanthine oxidase inhibitor. J Microbiol Biotechnol 2013;41:383-90. https://doi.org/10.4014/kjmb.1309.09002
  13. Hyun SH, Min JH, Kim AR, Kim HK, Lee JS. Isolation and diversity of yeasts from wild flowers in Ulleungdo and Yokjido, Korea. Kor J Mycol 2014;42:28-33. https://doi.org/10.4489/KJM.2014.42.1.28
  14. Hyun SH, Lee HB, Lee JS. Characteristics of unrecorded yeasts, Rhodosporidium fluviale, Rhodosporidium paludigenum, Candida sp. 80-J-3 and Kluyveromyces thermotolerans isolated from wild flowers in Korea. Kor J Mycol 2014;41:181-4. https://doi.org/10.4489/KJM.2013.41.3.181
  15. Cushman DW, Cheung HS. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem Pharmacol 1971;20:1637-48. https://doi.org/10.1016/0006-2952(71)90292-9
  16. Kim JK, Cha WS, Park JH, Oh SL, Cho YJ, Chun SS, Choi C. Inhibition effect against tyrosinase of condensed tannins from Korean green tea. Kor J Food Sci Technol 1997; 29:173-7.
  17. Kang MG, Yi SH and Lee JS. Production and characterization of a new ${\alpha}$-glucosidase inhibitory peptide from Aspergillus oryzae N159-1. Mycobiology 2013;41:149-54. https://doi.org/10.5941/MYCO.2013.41.3.149
  18. Lee JS, Choi YJ, Kwon SJ, Yoo JY, Chung DH. Screening and characterization of osmotolerant and gas-producing yeasts from traditional Doenjang and Kochujang. Food Biotechnol 1996;5:54-8.
  19. Kim NM, So SH, Lee SG, Song JE, Seo DS, Lee JS. Physiological functionality and enzyme activity of biomass from Pichia anomala grown on ginseng-steaming effluent. Mycobiology 2008;36:148-51. https://doi.org/10.4489/MYCO.2008.36.3.148
  20. Jang JH, Jeong SC, Kim JH, Lee YH, Ju YC, Lee JS. Characterization of a new antihypertensive angiotensin I-converting enzyme inhibitory peptide from Pleurotus cornucopiae. Food Chem 2011;127:412-8. https://doi.org/10.1016/j.foodchem.2011.01.010

Cited by

  1. Yeasts Diversity of Wild Flowers in Mountains of Korea and Their Physiological Functionalities vol.43, pp.3, 2015, https://doi.org/10.4489/KJM.2015.43.3.137
  2. Isolation and Diversity of Wild Yeasts from Some Cereals vol.43, pp.1, 2015, https://doi.org/10.4489/KJM.2015.43.1.64
  3. Isolation and Identification of Yeasts from Wild Flowers in Deogyu Mountain and their Physiological Functionalities vol.43, pp.1, 2015, https://doi.org/10.4489/KJM.2015.43.1.47
  4. Isolation and Identification of Yeasts from Wild Flowers Collected around Jangseong Lake in Jeollanam-do, Republic of Korea, and Characterization of the Unrecorded YeastBullera coprosmaensis vol.43, pp.3, 2015, https://doi.org/10.5941/MYCO.2015.43.3.266