한국균학회지 (The Korean Journal of Mycology)

  • 제42권2호
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  • Pages.159-164
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  • 2014
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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큰느타리(Pleurotus eryngii) 품종 판별을 위한 초위성체 유래 다중 표지 개발

Multiplex Simple Sequence Repeat (SSR) Markers Discriminating Pleurotus eryngii Cultivar

  • Im, Chak Han (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Kim, Kyung-Hee (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Je, Hee Jeong (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Ali, Asjad (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Kim, Min-Keun (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Joung, Wan-Kyu (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Lee, Sang Dae (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Shin, HyunYeol (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Ryu, Jae-San (Gyeongsangnam-do Agricultural Research and Extension Services)
  • 투고 : 2014.05.13
  • 심사 : 2014.06.26
  • 발행 : 2014.06.30
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초록

큰느타리 품종구분을 위한 마커의 개발을 위하여 큰느타리 전체 유전자 염기서열을 바탕으로 제작한 484개의 SSR마커를 사용하여 다형성 분석을 실시하였다. 그 결과 각 275개의 primer에서 다형성이 관찰되었다. 이 중 품종간에 다양한 패턴을 나타내는 5개의 마커를 최종 선발하였다. 이들 마커의 PIC 값은 0.6627에서 0.6848로 나타났고, 평균값은 0.6775였다. 이 결과를 밴드 이미지 인식 방법으로 dendrogram을 작성하였다. UPGMA 집괴분석 결과, 큰느타리 품종은 크게 Cluster 1과 Cluster 2로 구분되었다. SSR primer를 이용한 PCR 결과 나타나는 품종별 고유의 DNA 밴드를 품종특이적 마커로 개발하기 위하여, 선발된 마커중에서 SSR312과 SSR366, SSR178과 SSR 277 마커를 조합하여 초위성체 유래 다중 표지 세트를 개발하였다. Multiplex-SSR 마커의 사용을 통해 두번의 PCR 반응만으로 본 연구에서 사용된 12개의 큰느타리 품종을 구분할 수 있었다.

For development of a method for differentiation of Pleurotus eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotyping results. PCR using each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in the range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pairgroup method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into two clusters. Cluster I and II were comprised of four and eight cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivars and strains with 21 alleles and a PIC value of 0.9090. These results might be useful in providing an efficient method for the identification of P. eryngii cultivars with separate PCR reactions.

키워드

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  2. ) pp.2092-9323, 2018, https://doi.org/10.1080/12298093.2018.1538072
  3. Genome-Wide Characterization and Expression Analyses of Pleurotus ostreatus MYB Transcription Factors during Developmental Stages and under Heat Stress Based on de novo Sequenced Genome vol.19, pp.7, 2018, https://doi.org/10.3390/ijms19072052