International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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A Combination Method of CO2-Narcosis and Cold Treatment for Breaking Diapause of Bombus ignitus and Bombus terrestris Bumblebee Queens

  • Yoon, Hyung Joo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Lee, Kyeong Yong (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
  • Received : 2014.05.26
  • Accepted : 2014.06.15
  • Published : 2014.06.30
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Abstract

Bumblebees are important pollinators of crops and wildflowers. Bumblebees generally produce one generation per year. One of the key stages for year-round rearing of bumblebees is breaking diapause. To evaluate the effects of a combination method of $CO_2$-narcosis and cold treatment to break the diapause of B. ignitus and B. terrestris queens, we determined whether this method affected their ability to establish a colony after the diapause break. The diapause treatment regimes that were utilized were $CO_2$ ($CO_2$-narcosis), CT-1M (cold treatment at $5^{\circ}C$ for 1 mo), CT-1M-$CO_2$ ($CO_2$-narcosis after cold treatment for 1 mo), CT-2M-$CO_2$ ($CO_2$-narcosis after cold treatment for 2 mo), CT-2M (cold treatment for 2 mo), CT-2.5M-$CO_2$ ($CO_2$-narcosis after cold treatment for 2.5 mo) and CT-2.5M (cold treatment at $5^{\circ}C$ for 2.5 mo). In view of the effects on the colony developmental characteristics of B. ignitus queens, the most favorable diapause treatment was CT-1M-$CO_2$. A combination method of $CO_2$-narcosis and cold temperature treatment yielded better results than that of single $CO_2$-narcosis or cold temperature treatment on the colony development of diapause-broken B. ignitus queens. In the case of B. terrestris queens, we concluded that a combination method of $CO_2$ and cold temperature treatment yielded better results than that of a single cold-temperature (up to 2 mo) treatment. In conclusion, the findings of the present study indicated that the combined application of $CO_2$ and cold temperature was a favorable method for the colony development of diapause-broken B. ignitus and B. terrestris queens compared with only $CO_2$-narcosis or cold temperature treatments. A combination method of $CO_2$ and cold treatment reduced the side effect of $CO_2$-narcosis and shortened the duration of cold treatment by at least 1 mo.

Keywords

References

  1. Alford DV (1969) A study of the hibernation of bumblebees (Hymenoptera: Bombidae) in southern England. J Anim Ecol 38, 149-170. https://doi.org/10.2307/2743
  2. Alford DV (1975) Bumblebees. Davies-Poynter, London. pp. 352.
  3. Andrewartha HG (1952) Diapause in relation to the ecology of insects. Bio Rev 27, 50-107. https://doi.org/10.1111/j.1469-185X.1952.tb01363.x
  4. Asada S, Ono M (2000) Difference in colony development of two Japanese bumblebee, Bombus hypocrita and B. ignitus (Hymenoptera: Apidae). Appl Entomol Zool 35, 597-603. https://doi.org/10.1303/aez.2000.597
  5. Beekman M, van Stratum P, Lingeman R (1998) Diapause survival and post-diapause performance in bumblebee queens (Bombus terrestris). Entomol Experi Appli 89, 207-214. https://doi.org/10.1046/j.1570-7458.1998.00401.x
  6. Dag A, Kammer Y (2001) Comparison between the effectiveness of honeybee (Apis millifera) and bumblebee (Bombus terrestris) as pollinators of greenhouse sweet pepper (Capsicum annuum). Am Bee J 141, 447-448.
  7. Denlinger DL (2002) Regulation of diapauses. Annu Rev Entomol 47, 93-122. https://doi.org/10.1146/annurev.ento.47.091201.145137
  8. Duchateau MJ, Velthuis HHW (1988) Development and reproductive strategies in Bombus terrestris colonies. Behavior 107, 186-207. https://doi.org/10.1163/156853988X00340
  9. Free JB (1993) Insect pollination of crops. 2nd ed., 684 pp. Academic Press, London.
  10. Greenleaf S, Kremen C (2006) Wild bee species increase tomato production but respond differently to surrounding land use in Northern California. Biol Conserv 133, 81-87. https://doi.org/10.1016/j.biocon.2006.05.025
  11. Heinrich B (1979) Bumblebee economics. Harvard University Press, Cambridge, MA.
  12. Hoem SN (1972) Weight and life length of hibernation bumblebee queens (Hymenoptera: Bombidae) under controlled conditions. Ent Scand 3, 313-320. https://doi.org/10.1163/187631272X00184
  13. Horber E (1961) Beitrag zur Domestifikationsversuche mit Hummeln (Bombus latr). Albredht Thaer-Arch 5, 282-304.
  14. Larrere M, Lavenseau L, Tasei JN, Couillaud F (1993) Juvenile hormone biosynthesis and diapause termination in Bombus terrestris. Invertbr Reprod Dev 23, 7-14. https://doi.org/10.1080/07924259.1993.9672288
  15. Mansigh A (1971) Physiological classification of dormancy in insects. Can Entomol 103, 983-1009. https://doi.org/10.4039/Ent103983-7
  16. Michener CD (2000) The bees of the world. Baltimore: Johns Hopkins University Press.
  17. Minitab Incorporated Company (2000) Minitab user's guide, Minitab Inc., USA.
  18. Morandin LA, Winston ML (2005) Wild bee abundance and seed production in conventional, organic, and genetically modified canola. Ecol Appl 15, 871-881. https://doi.org/10.1890/03-5271
  19. Nicolas G (1989) Immiediate and latent effects of carbon dioxide on insects. Ann Rev Entomol 34, 97-116. https://doi.org/10.1146/annurev.en.34.010189.000525
  20. Pomeroy N, Plowright RC (1979) Larval injection following $CO_2$ narcosis of bumblebees (Hymenoptera : Apidae). J Kansas Entomol Soc 52, 215-217.
  21. Roseler PF (1985) A technique for year-round rearing of Bombus terrestris (Apidae, Bombini) colonies in captivity. Apidolo 16, 165-170. https://doi.org/10.1051/apido:19850206
  22. Roseler PF, Roseler I (1984) Effects of carbon dioxide and brain cauterization on corpora allata activity and oogenesia in bumblebees(Bombus hypnorum and Bombus terrestris). Zool Jb Physiol 88, 237-246.
  23. Tasei JN (1994) Effect of different narcosis procedures on initiating oviposition of prediapausing Bombus terrestris L. queens. Entomol Exp Appl 72, 273-279. https://doi.org/10.1111/j.1570-7458.1994.tb01827.x
  24. van den Eijnde JA, de Ruijter, van der Steen J (1991) Method for rearing Bombus terrestris continuously and the production of bumblebee colonies for pollination purposes. Acta Horticulturae 288, 154-158.
  25. Winfree R, Griswold T, Kremen C (2007) Effect of human disturbance on bee communities in a forested ecosystem. Conserv Biol 21, 213-223. https://doi.org/10.1111/j.1523-1739.2006.00574.x
  26. Yoon HJ, Kim SE, Kim YS (2002) Temperature and humidity favorable for colony development of the indoor-reared bumblebee, Bombus ignitus. Appl Entomol Zool 37, 419-423. https://doi.org/10.1303/aez.2002.419
  27. Yoon HJ, Kim SE (2002) Facilitating effects of helpers on oviposition and colony development of bumblebee queen, Bombus ignitus. Korean J Appl Entomol 41, 239-245.
  28. Yoon HJ, Kim SE, Lee SB, Park IG (2003) Effect of $CO_2$-treatment on oviposition and colony development of the bumblebee, Bombus ignitus. Korean J. Appl. Entomol. 42, 139-144.
  29. Yoon HJ, Kim SE, Kim YS, Lee SB (2004a) Colony developmental characteristics of the bumblebee queen Bombus ignitus by the first ovipositon day. Int J Indust Entomol 8, 139-143.
  30. Yoon HJ, Lee SB, Kim SE, Seol KL (2004b) The flight of the bumblebee queen, Bombus terrestris, after diapause termination affects to ovipositon and colony development. Int J Indust Entomol 9, 241-247.
  31. Yoon HJ, Lee KY, Kim MA, Ahn MY, Park IG (2013) Optimal cold temperature for the artificial hibernation of Bombus terrestris queen bumblebees. Int J Indust Entomol 26, 120-130.

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