Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Increased Resistance to Quinolones in Streptococcus parauberis and Development of a Rapid Assay for Detecting Mutations in Topoisomerase Genes

Streptococcus parauberis의 퀴놀론 내성 증가와 Topoisomerase 유전자에서의 돌연변이 신속 분석

  • Kim, So Yeon (National Fishery Products Quality Management Service) ;
  • Kim, Young Chul (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Jeong, Seo Kyung (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Jun, Lyu Jin (School of Marine Biomedical Sciences, Jeju National University) ;
  • Jin, Ji Woong (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Jeong, Hyun Do (Department of Aquatic Life Medicine, Pukyong National University)
  • 김소연 (국립수산물품질관리원) ;
  • 김영철 (부경대학교 수산생명의학과) ;
  • 정서경 (부경대학교 수산생명의학과) ;
  • 전려진 (제주대학교 해양의생명과학부) ;
  • 진지웅 (부경대학교 수산생명의학과) ;
  • 정현도 (부경대학교 수산생명의학과)
  • Received : 2014.05.14
  • Accepted : 2014.06.12
  • Published : 2014.06.30
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Abstract

To investigate the acquisition of quinolone resistance, we examined mutations in the quinolone resistance-determining region (QRDR) of type II topoisomerase genes in ciprofloxacin (CIP)-resistant clinical isolates and in vitro mutants of Streptococcus parauberis. The CIP-resistant clinical isolates had one base change responsible for a Ser-79${\rightarrow}$Thr in the QRDR of parC. However, the CIP-resistant in vitro mutants had an altered QRDR of parC (Ser-79${\rightarrow}$Ile) that differed from that of the isolates. None of the CIP-resistant S. parauberis clinical isolates or in vitro mutants exhibited amino acid changes in gyrA or gyrB. However, even though involvement in the increased resistance was not clear, an Arg-449${\rightarrow}$Ser mutation outside of the QRDR of parE was detected in CIP-resistant mutant 2P1. These results suggest that the topoisomerase IV gene, parC (and possibly parE, as well), is the primary ciprofloxacin target in S. parauberis. Additionally we established a high-resolution melting (HRM) assay capable of detecting the dominant mutation in four type II topoisomerase genes conferring ciprofloxacin resistance. These rapid and reliable assays may provide a convenient method of surveillance for genetic mutations conferring antibiotic resistance.

Keywords

References

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