Korean Journal of Materials Research (한국재료학회지)

Materials Research Society of Korea (한국재료학회)
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In Vitro and In Vivo Evaluation of Composite Scaffold of BCP, Bioglass and Gelatin for Bone Tissue Engineering

  • Kim, Woo Seok (Department of Physiology, Soonchunhyang University) ;
  • Nath, Subrata Deb (Institute of Tissue Regeneration, Soonchunhyang University) ;
  • Bae, Jun Sang (Institute of Tissue Regeneration, Soonchunhyang University) ;
  • Padalhin, Andrew (Department of Regenerative Medicine, School of Medicine, Soonchunhyang University) ;
  • Kim, Boram (Department of Regenerative Medicine, School of Medicine, Soonchunhyang University) ;
  • Song, Myeong Jin (Department of Regenerative Medicine, School of Medicine, Soonchunhyang University) ;
  • Min, Young Ki (Department of Physiology, Soonchunhyang University)
  • Received : 2014.04.11
  • Accepted : 2014.05.23
  • Published : 2014.06.27
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Abstract

In this experiment, a highly porous scaffold of biphasic calcium phosphate (BCP) was prepared using the spongereplica method. The BCP scaffold was coated with 58S bioactive glass (BG) and sintered for a second time. The resulting scaffold was coated with gelatin (Gel) and cross-linked with [3-(3-dimethyl aminopropyl) carbodiimide] and N-Hydroxysuccinamide (EDC-NHS). The initial average pore size of the scaffold ranged from 300 to $700{\mu}m$, with more than 85 % porosity. The coating of BG and Gel had a significant effect on the scaffold-pore size, decreasing scaffold porosity while increasing mechanical strength. The material and surface properties were evaluated by means of several experiments involving scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) and X-ray diffraction (XRD). Cytotoxicity was evaluated using MTT assay and confocal imaging of MC3T3-E1 pre-osteoblast cells cultured in vitro. Three types of scaffold (BCP, BCP-BG and BCP-BG-Gel) were implanted in a rat skull for in vivo evaluation. After 8 weeks of implantation, bone regeneration occurred in all three types of sample. Interestingly, regeneration was found to be greater (geometrically and physiologically) for neat BCP scaffolds than for two other kinds of composite scaffolds. However, the other two types of scaffolds were still better than the control (i.e., defect without treatment).

Keywords

References

  1. J. R. Jones, A. R. Boccaccini, Cellular ceramics in biomedical applications: tissue engineering, M. Scheffler, P. Colombo (Eds.), Cellular ceramics: structure, manufacturing, processing and applications, Wiley-VCH Verlag GmbH & Co. KgaA, Weinheim, p. 550-573 (2005).
  2. K. Rezwan, Q. Z. Chen, J. J. Blaker and A. R. Boccaccini, Biomaterials., 27(18), 3413 (2006). https://doi.org/10.1016/j.biomaterials.2006.01.039
  3. J. S. Hollister, Nature Mater., 4(7), 518 (2005). https://doi.org/10.1038/nmat1421
  4. S. Bose, M. Roy, and A. Bandyopadhyay, Trends in Biotechnol., 30(10), 546 (2012). https://doi.org/10.1016/j.tibtech.2012.07.005
  5. M. N. Rahaman, D. E. Day, B. S. Bal, Q. Fu, S. B. Jung, L. F. Bonewald and A. P. Tomsia, Acta Biomater., 7(6), 2355 (2011). https://doi.org/10.1016/j.actbio.2011.03.016
  6. D. Mondal, S. Sora and B. T. Lee, J. Mater. Sci., 48(5), 1863 (2013). https://doi.org/10.1007/s10853-012-6956-3
  7. A. Sadiasa, S. K. Sarkar, R. A. Franco, Y. K. Min and B. T. Lee, J. Biomater. Appl., 28(5), 739 (2014). https://doi.org/10.1177/0885328213478256
  8. M. M. Pereira, J. R. Jones and L. L. Hench, Advan. Appl. Ceram., 104(1), 35 (2005). https://doi.org/10.1179/174367605225011034
  9. R. C. Bielby, I. S. Christodoulou, R. S. Pryce, W. J. Radford, L. L. Hench and J. M. Polak, Tissue. Engi., 10(7-8), 1018 (2004). https://doi.org/10.1089/ten.2004.10.1018
  10. M. Okamoto and B. John, Prog. Polym. Sci., 38(10), 1487 (2013). https://doi.org/10.1016/j.progpolymsci.2013.06.001
  11. J. Y. Chang, J. H. Lin, C. H. Yao, J. H. Chen, T. Y. Lai and Y. S. Chen, Macromol. Biosci., 7(4), 500 (2007). https://doi.org/10.1002/mabi.200600257
  12. M. Ikenaga, P. Hardouin, J. Lemaitre, H. Andrianjatovo and B. Flautre, J. Biomed. Mater. Res. Part A, 40(1), 139 (1998). https://doi.org/10.1002/(SICI)1097-4636(199804)40:1<139::AID-JBM16>3.0.CO;2-J
  13. H. Guo, J. Su, J. Wei, H. Kong and C. Liu, Acta Biomater., 5(1), 268 (2009). https://doi.org/10.1016/j.actbio.2008.07.018
  14. A. J. Ambard and L. Mueninghoff, J. Prosthodont., 15(5), 321 (2006). https://doi.org/10.1111/j.1532-849X.2006.00129.x
  15. A. Sadiasa, D. W. Jang, S. D. Nath, H. S. Seo, H. M. Yang and B. T. Lee, Kor. J. Mater. Res., 23(3), 168 (2013). https://doi.org/10.3740/MRSK.2013.23.3.168
  16. S. D. Nath, N. T. Linh, A. Sadiasa and B. T. Lee, J. Biomater. Appl., 28(8), 1151 (2014). https://doi.org/10.1177/0885328213499272
  17. G. M. Calori, E. Mazza, M. Colombo and C. Ripamonti, Injury, 42, 56 (2011). https://doi.org/10.1016/j.injury.2011.06.011
  18. B. T. Lee, M. H. Youn, R. K. Paul, K. Lee and H. Y. Song, Mater. Chem. Phys., 104(2), 249 (2007). https://doi.org/10.1016/j.matchemphys.2007.02.009
  19. A. Balamurugan, G. Sockalingum, J. Michel, J. Fauré, V. Banchet, L. Wortham, S. Bouthors, D. L.aurent-Maquin and G. Balossier, Mater. Lett., 60(29), 3752 (2006). https://doi.org/10.1016/j.matlet.2006.03.102
  20. S. Sakai, K. Hirose, K. Taguchi, Y. Ogushi and K. Kawakami, Biomaterials., 30(20), 3371 (2009). https://doi.org/10.1016/j.biomaterials.2009.03.030
  21. M. S. Kim, D. W. Jang, Y. K. Min, H. M. Yang, H. Y. Song and B. T. Lee, Mater. Sci. Forum, 654, 2245 (2010)
  22. V. Karageorgiou and D. Kaplan, Biomaterials, 26(27), 5474 (2005). https://doi.org/10.1016/j.biomaterials.2005.02.002
  23. S. I Roohani-Esfahani, S. Nouri-Khorasani, Z. F. Lu, R. C. Appleyard and H. Zreiqat, Acta Biomater., 7(3), 1307 (2011). https://doi.org/10.1016/j.actbio.2010.10.015
  24. A. Odgaard, Bone, 20(4), 315 (1997). https://doi.org/10.1016/S8756-3282(97)00007-0
  25. M. J. Luyn, P. B. Wachem, L. H. Damink, P. J. Dijkstra, J. Feijen and P. Nieuwenhuis, Biomaterials., 13, 1017 (1992). https://doi.org/10.1016/0142-9612(92)90153-F
  26. L. H. Damink, P. J. Dijkstra, M. J. Luyn, P. B. Wachem, P. Nieuwenhuis and J. Feijen, Biomaterials., 17, 765 (1996). https://doi.org/10.1016/0142-9612(96)81413-X
  27. M. A. Gilles, A. Q. Hudson and C. L. Borders Jr, Anal. Biochem., 184, 244 (1990). https://doi.org/10.1016/0003-2697(90)90675-Y