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비경의 오수혈에 대한 830 nm 레이저침이 DSS로 유발된 흰쥐의 대장염에 미치는 영향

Laser Acupuncture Treatment on the Five Transport Points of the Spleen Meridian in Dextran-Sulfate-Sodium-Induced-Colitis in Rats

  • 최동희 (동신대학교 한의과대학 경혈학교실) ;
  • 김왕인 (동신대학교 한의과대학 경혈학교실) ;
  • 김미래 (동신대학교 한의과대학 경혈학교실) ;
  • 윤대환 (동신대학교 한의과대학 경혈학교실) ;
  • 나창수 (동신대학교 한의과대학 경혈학교실)
  • Choi, Dong-Hee (Department of Meridian & Acupoint, College of Oriental Medicine, Dongshin University) ;
  • Kim, Wang-In (Department of Meridian & Acupoint, College of Oriental Medicine, Dongshin University) ;
  • Kim, Mi-Rea (Department of Meridian & Acupoint, College of Oriental Medicine, Dongshin University) ;
  • Youn, Dae-Hwan (Department of Meridian & Acupoint, College of Oriental Medicine, Dongshin University) ;
  • Na, Chang-Su (Department of Meridian & Acupoint, College of Oriental Medicine, Dongshin University)
  • 투고 : 2014.03.21
  • 심사 : 2014.05.13
  • 발행 : 2014.06.27

초록

Objectives : The purpose of this study is to compare the effects of laser acupuncture to the 830 nm on the five transport points with the spleen meridian for treatment to intestinal disease in rat with dextran sulfate sodium(DSS)-induced colitis. Methods : Colitis was induced by DSS for 20 days. The laser therapy on the five transport points of spleen meridian (Laser Well Point-SP1(L-WE), Laser Brook Point-SP2(L-BR), Laser Stream Point-SP3(L-ST), Laser River Point-SP5(L-RI) and Laser Sea Point-SP9(L-SE) was practiced twice a week for 5 times. Colon length was measured using a measuring point. Histological evaluation of colitis was conducted by hematoxylin and eosin(H&E) staining. Reverse transcription polymerase chain reaction(RT-PCR) was determined using western blotting and quantitative reverse-transcriptase polymerase chain reaction, respectively. Results: Colon length increased significantly L-BR and L-ST points after 5 times of therapy. Damage to the colonic mucosa is an integral feature of the DSS model, so control colonic mucosa tissue was damaged in the areas of ulceration resulting in complete epithelial loss. However histological damage decreased on the epithelial lining at all points. Cyclooxygenase(COX)-2 concentrations decreased in all points groups and Interferon(IFN)-${\gamma}$ increased in L-WE, L-BR, L-RI and L-SE points but L-ST was decreased when compared with control. White blood cell(WBC) and neutrophils(NE) decreased after the fifth acupuncture on the all points. But hemoglobin(HGB) increased after the fifth acupuncture on the L-WE, L-BR, L-ST and L-RI points. Also Mean corpuscular hemoglobin(MCH) and Mean corpuscular hemoglobin concentration(MCHC) decreased after the fifth acupuncture on the all points. Conclusions: The present study indicated that five transport points of the spleen meridian can prevent the development of DSS-induced colitis in rat. Thereby suggesting that should be available for decreasing DSS-induced inflammation in a colonic mucosa of tissue.

키워드

참고문헌

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피인용 문헌

  1. The Effects of Pharmacopuncture Therapy on the (ST) for DSS-induced Colitis in Rats vol.32, pp.2, 2015, https://doi.org/10.13045/acupunct.2015021
  2. Trends of Studies in Korean Journal of Acupuncture vol.33, pp.1, 2015, https://doi.org/10.14406/acu.2016.002
  3. The Effects of Pulsatilla Koreana NAKAI Pharmacopuncture Therapy on the Chunchu (ST25) for Dextran Sulfate Sodium-Induced Colitis in Mice vol.35, pp.1, 2018, https://doi.org/10.13045/jar.2018.00024