대한본초학회지 (The Korea Journal of Herbology)

  • 제29권3호
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  • Pages.79-85
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  • 2014
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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케어스웰을 이용한 사이토카인 억제 효과에 대한 연구

The Study of Cytokine Inhibitory Effect using Careswell

  • 하효철 (대구한의대학교 식품약리학과) ;
  • 오사랑 (대구한의대학교 한약재약리학과) ;
  • 이지혜 (대구한의대학교 한약재약리학과) ;
  • 정지욱 (대구한의대학교 한약재약리학과)
  • Ha, Hyocheol (Department of Herbal Food Science, Daegu Haany University) ;
  • Oh, Sarang (Department of herbal medicine pharmacology, Daegu Haany University) ;
  • Lee, Jihye (Department of herbal medicine pharmacology, Daegu Haany University) ;
  • Jung, Ji Wook (Department of herbal medicine pharmacology, Daegu Haany University)
  • 투고 : 2014.04.25
  • 심사 : 2014.05.14
  • 발행 : 2014.05.30
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초록

Objectives : In this study, we attempted to evaluate the effects of Careswell on human mast cell-mediated allergy inflammation in vitro and pruritogen-induced scratching behavior in vivo. Method : The Careswell was extract by distilled water. The anti-itching effects of Careswell were investigated on the compound 48/80 ($50{\mu}g/kg$) or histamine ($100{\mu}g/kg$) induced scratching behavior male ICR mice for 30 min by an observer blind. Terfenadine (10 mg/kg) was used as a positive control drug. The cell toxicity of Careswell was determined by 3-(4,5-dimethylthiazole-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The regulatory effect of Careswell on interleukin (IL)-6 and tumor necrosis factor (TNF)-${\alpha}$ levels was determined by enzyme-linked immunosorbent assay (ELISA) in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI) stimulated human mast cells (HMC-1). Also, we evaluated the effect of Careswell on PMACI induced the activation of Nuclear factor-kappa B (NF-${\kappa}B$) into nucleus by Western blot analysis. Result : The results revealed that the oral administration of Careswell (200 mg/kg, p.o.) attenuated the compound 48/80 or histamine-induced scratching behavior in mice. We showed that Careswell significantly reduced the PMACI-induced the production of IL-6 (0.5-1 mg/ml) and TNF-${\alpha}$ (0.1-1 mg/ml). Additionally, Careswell significantly inhibited the activation of NF-${\kappa}B$ in PMACI-stimulated HMC-1. Conclusion : Collectively, the findings of this study provide us with a novel insight into the pharmacological actions of Careswell as a potential molecule for use in the treatment of allergic inflammation diseases.

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