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마치현(馬齒莧)의 급성 췌장염 보호 효과

Protective effects of Portulaca oleracea against cerulein-induced acute pancreatitis

  • 곽태신 (원광대학교 한의과대학 본초학교실) ;
  • 김동구 (원광대학교 한의과대학 본초학교실) ;
  • 김주영 (계명대학교 의과대학 예방의학과) ;
  • 배기상 (원광대학교 한방체액조절연구센터) ;
  • 최선복 (원광대학교 한의학전문대학원 BK21 plus 사업팀) ;
  • 조일주 (원광대학교 한의과대학 본초학교실) ;
  • 신준연 (원광대학교 한의과대학 본초학교실) ;
  • 이성곤 (원광대학교 한의과대학 본초학교실) ;
  • 김명진 (원광대학교 한의과대학 본초학교실) ;
  • 김민준 (원광대학교 한의과대학 본초학교실) ;
  • 송호준 (원광대학교 한의과대학 본초학교실) ;
  • 박성주 (원광대학교 한의과대학 본초학교실)
  • Gwak, Tae-Sin (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Dong-Goo (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Ju-Young (Dept. of Preventive Medicine, School of Medicine, Keimyung University) ;
  • Bae, Gi-Sang (Hanbang Body-fluid Research Center, Wonkwang University) ;
  • Choi, Sun-Bok (BK21 plus team, Professional graduate school of Oriental medicine, Wonkwang University) ;
  • Jo, Il-Joo (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Shin, Joon-Yeon (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Sung-Kon (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Myoung-Jin (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Min-Jun (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Song, Ho-Joon (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Park, Sung-Joo (Dept. of Herbology, College of Oriental Medicine, Wonkwang University)
  • 투고 : 2014.04.25
  • 심사 : 2014.05.14
  • 발행 : 2014.05.30

초록

Objective : Portulaca oleracea (PO) has been used as an important traditional medicine for inflammatory and bacterial diseases in East Asia. However, the protective effects of PO on acute pancreatitis (AP) is not well-known. Therefore, this study was performed to identify the anti-inflammatory and prophylactic effects of PO on cerulein-induced AP. Methods : AP was induced in mice via intraperitoneal injection of supramaximal concentrations of the stable cholecystokinin analogue cerulein ($50{\mu}g/kg$) given every hour for 6 times. Water extracts of PO (100, 300, or 500 mg/kg) was administrated intra-peritoneally 1 h prior to the first injection of cerulein. The mice were killed at 6 h after the final cerulein injection. Pancreas and lung were rapidly removed for morphologic and histochemical examination, myeloperoxidase (MPO) assay. Blood samples were taken to determine serum amylase and lipase activities. Results : Administration of PO significantly inhibited pancreatic weight/body weight ratio, pancreas and lung histological injury. And MPO activity which indicates neutrophil infiltration was inhibited by PO extracts on cerulein-induced pancreatitis. In addition, PO administration inhibited digestive enzymes such as serum amylase and lipase activity on cerulein-induced pancreatitis. Conclusion : Our results could suggest that pre-treatment of PO reduces the severity of cerulein-induced AP, thereby, PO could be used as a protective agent against AP. Also, this study could give a clinical basis that PO could be a drug or agent to prevent AP.

키워드

참고문헌

  1. Kingsnorth A, O'Reilly D. Acute pancreatitis. Br Med J. 2006 ; 332 : 1072-6. https://doi.org/10.1136/bmj.332.7549.1072
  2. Steinberg W, Tenner S. Acute pancreatitis. N Engl J Med. 1994 ; 330 : 1198-210. https://doi.org/10.1056/NEJM199404283301706
  3. Beger HG, Rau B, Isenmann R. Natural history of necrotizing pancreatitis. Pancreatology. 2003 ; 3(2) : 93-101.
  4. Yoon YK, Ji JH, Mun BS. Hypertriglyceridemia-induced pancreatitis. Korean J Gastroenterol. 2008 ; 51 : 309-13.
  5. Wang GJ, Gao CF, Wei D, Wang C, Ding SQ. Acute pancreatitis: Etiology and common pathogenesis. World J Gastroenterol. 2009 ; 15 : 1427-30. https://doi.org/10.3748/wjg.15.1427
  6. Baron TH, Morgan DE. Acute necrotizing pancreatitis. N Engl J Med. 1999 ; 340 : 1412-7. https://doi.org/10.1056/NEJM199905063401807
  7. Ho HS, Frey CF. Gastrointestinal and pancreatic complications associated with severe pancreatitis. Arch Surg. 1995 ; 130 : 817-22. https://doi.org/10.1001/archsurg.1995.01430080019002
  8. Kota SK, Kota SK, Jammula S, Krishna SV, Modi KD. Hypertriglyceridemia-induced recurrent acute pancreatitis: A case-based review. Indian J Endocrinol Metab. 2012 ; 16 : 141-3. https://doi.org/10.4103/2230-8210.91211
  9. Kimura W, Mossner J. Role of hypertriglyceridemia in the pathogenesis of experimental acute pancreatitis in rats. Int J Pancreatol. 1996 ; 20 : 177-84. https://doi.org/10.1007/BF02803766
  10. Jenkins DJ, Kendall CW, Augustin LS, Franceschi S, Hamidi M, Marchie A, Jenkins AL, Axelsen M. Glycemic index: Overview of implications in health and disease. Am J Clin Nutr. 2002 ; 76 : 266S-73S. https://doi.org/10.1093/ajcn/76.1.266S
  11. Barter PJ, Rye KA. Cardioprotective properties of fibrates: which fibrate, which patients, what mechanism? Circulation. 2006 ; 113 : 1553-5. https://doi.org/10.1161/CIRCULATIONAHA.105.620450
  12. Carlson LA. Nicotinic acid: The broad-spectrum lipid drug. A 50 th anniversary review. J Intern Med. 2005 ; 258 : 94-114. https://doi.org/10.1111/j.1365-2796.2005.01528.x
  13. Sood A, Arora R. Mechanisms of flushing due to niacin and abolition of these effects. J Clin Hypertens (Greenwich). 2009 ; 11 : 685-9. https://doi.org/10.1111/j.1559-4572.2008.00050.x
  14. Carlson LA. Niaspan, the prolonged release preparation of nicotinic acid (niacin), the broad-spectrum lipid drug. Int J Clin Pract. 2004 ; 58 : 706-13. https://doi.org/10.1111/j.1368-5031.2004.00233.x
  15. Covington MB. Omega-3-Fatty Acids. Am Fam Physician. 2004 ; 70 : 133-40.
  16. Nissen SE, Wolski K, Topol EJ. Effect of muraglitazar on death and major adverse cardiovascular events in patients with type 2 diabetes mellitus. JAMA. 2005 ; 294 : 2581-6. https://doi.org/10.1001/jama.294.20.joc50147
  17. Gorelick FS, Adler G, Kern HF. Cerulein-induced pancreatitis. In: Go VLW, DiMagno ER, Gardner JD, Lebenthal E, Reber HA, Scheele GA, eds. The pancreas. 2nd ed. New York : Raven. 1993 : 501-26.
  18. Bhatia M. Novel therapeutic targets for acute pancreatitis and associated multiple organ dysfunction syndrome. Curr Drug Targets Inflamm Allergy. 2002 ; 1(4) : 343-51. https://doi.org/10.2174/1568010023344517
  19. Monga A, Arora A, Makkar RP, Gupta AK. Hypertriglyceridemia-induced acute pancreatitis-treatment with heparin and insulin. Indian J Gastroenterol. 2003 ; 22 : 102-3.
  20. Piolot A, Nadler F, Cavallero E, Coquard JL, Jacotot B. Prevention of recurrent acute pancreatitis in patients with severe hypertriglyceridemia: Value of regular plasmapheresis. Pancreas. 1996 ; 13 : 96-9. https://doi.org/10.1097/00006676-199607000-00013
  21. Etemad B, Whitcomb DC. Chronic pancreatitis: Diagnosis, classification, and new genetic developments. Gastroenterology. 2001 ; 120 : 682-707. https://doi.org/10.1053/gast.2001.22586
  22. Fernandez-del Castillo C, Harringer W, Warshaw AL, Vlahakes GJ, Koski G, Zaslavsky AM, Rattner DW. Risk factors for pancreatic cellular injury after cardiopulmonary bypass. N Engl J Med. 1991 ; 325 : 382-87. https://doi.org/10.1056/NEJM199108083250602
  23. Tadeo Ruiz G, Ais Conde G, Vazquez Santos P, Manzanares JJ. Pancreatitis caused by hypercalcemia of malignant origin. Rev Esp Enferm Dig. 1996 ; 88 : 640-2.
  24. Bae GS. The effects of Nardostachys jatamansi against choline deficient ethionine supplement diet-induced severe acute pancreatitis. Graduate School of Wonkang University. 2011.
  25. Waele BD, Smitz J, Willems G. Recurrent pancreatitis secondary to hypercalcemia following vitamin D poisoning. Pancreas. 1989 ; 4 : 378-80. https://doi.org/10.1097/00006676-198906000-00017
  26. Gaur S. Sarcoidosis manifested as hypercalcemic pancreatitis. South Med J. 2001 ; 94 : 939-40. https://doi.org/10.1097/00007611-200194090-00026
  27. Davies M, Klimiuk PS, Adams PH, Lumb GA, Large DM, Anderson DC. Familial hypocalciuric hypercalcemia and acute pancreatitis. Br Med J (Clin Res Ed). 1981 ; 282 : 1023-5. https://doi.org/10.1136/bmj.282.6269.1023
  28. Stemp LI. Pancreatic injury after cardiopulmonary bypass. N Engl J Med. 1992 ; 326 : 572-4. https://doi.org/10.1056/NEJM199202203260814
  29. Karimi G, Hosseinzadeh H, Ettehad N. Evaluation of the gastric antiulcerogenic effects of Portulaca olerracea L. extracts in mice. Phytother Res. 2004 ; 18(6) : 484-7. https://doi.org/10.1002/ptr.1463
  30. Rashed AN, Afifi FU, Disi AM. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1. J Ethnopharmacol. 2003 ; 88 : 131-6. https://doi.org/10.1016/S0378-8741(03)00194-6
  31. Kim CH. The inhibitory effects of Portulaca oleracea L. on HCl-ethanol induced gastritis in rats. Kor J Herbology. 2009 ; 24 : 41-7.
  32. Lim YY, Quaha EPL. Antioxidant properties of different cultivars of Portulaca oleracea. Food Chem. 2007 ; 103 : 734-40. https://doi.org/10.1016/j.foodchem.2006.09.025
  33. Hassan A, Ibrahim A, Mbodji K, Coeffier M, Ziegler F, Bounoure F, Chardigny JM, Skiba M, Savoye G, Dechelotte P, Marion-Letellier R. An $\alpha$-linolenic acid-rich formula reduces oxidative stress and inflammation by regulating NF-${\kappa}B$ in rats with TNBS-induced colitis. J Nutr. 2010 ; 140(10) : 1714-21. https://doi.org/10.3945/jn.109.119768
  34. Kim TH, Bae GS, Oh HJ, Kim MS, Park KC, Koo BS, Kim BJ, Yang YS, Park DE, Lee JH, Seo SW, Shin YK, Yun KJ, Sohn DH, Kim HJ, So HS, Park RK, Song HJ, Park SJ. 2',4',6'-Tris(methoxymethoxy) chalcone (TMMC) attenuates the severity of cerulein-induced acute pancreatitis and associated lung injury. Am J Physiol Gastrointest Liver Physiol. 2011 ; 301(4) : 694-706. https://doi.org/10.1152/ajpgi.00210.2010
  35. Bae GS, Kim MS, Park KC, Koo BS, Jo IJ, Choi SB, Lee DS, Kim YC, Kim TH, Seo SW, Shin YK, Song HJ, Park SJ. Effect of biologically active fraction of Nardostachys jatamansi on cerulein-induced acute pancreatitis. World J Gastroenterol. 2012 ; 18(25) : 3223-34.
  36. Cope O, Culver PJ, Mixter CG Jr, Nardi GL. Pancreatitis, a diagnostic clue to hyperparathyroidism. Ann Surg. 1957 ; 145 : 857-63. https://doi.org/10.1097/00000658-195706000-00007
  37. Thomson JC, Render HR, Ramus NI, Villar HV, Rayford PL. Cholecystokinin metabolism in man and dogs. Ann Surg. 1975 ; 182 : 496-504. https://doi.org/10.1097/00000658-197510000-00015
  38. Panteghini M, Pagani F, Bonora R. Clinical and analytical evaluation of a continuous enzymatic method for measuring pancreatic lipase activity. Clin Chem. 1993 ; 39(2) : 304-8.
  39. Granger J, Remick D. Acute pancreatitis : models, markers, nad mediators. Shock. 2005 ; 24(1) : 45-51. https://doi.org/10.1097/01.shk.0000191413.94461.b0
  40. Colgan SP, Ehrentraut SF, Glover LE, Kominsky DJ, Campbell EL. Contributions of neutrophils to resolution of mucosal inflammation. Immunol Res. 2013 ; 55(1-3) : 75-82 https://doi.org/10.1007/s12026-012-8350-2
  41. Interiano B, Stuard ID, Hyde RW. Acute respiratory distress syndrome in pancreatitis. Ann Int Med. 1972 ; 77 : 923-6. https://doi.org/10.7326/0003-4819-77-6-923
  42. Guice KS, Oldham KT, Johnson KJ, Kunkel RG, Morganroth ML, Ward PA. Pancreatitits-induced acute lung injury, an ARDS model. Ann Surg. 1988 ; 208(1) : 71-7. https://doi.org/10.1097/00000658-198807000-00010
  43. Jung WS, Chae YS, Kim DY, Seo SW, Park HJ, Bae GS, Kim TH, Oh HJ, Yun KJ, Park RK, Kim JS, Kim EC, Hwang SY, Park SJ, Song HJ. Gardenia jasminoides protects against cerulein-induced acute pancreatitis. World J Gastroenterol. 2008 ; 14(40) : 6188-94. https://doi.org/10.3748/wjg.14.6188
  44. Heath DI, Cruickshank A, Gudgeon AM, Jehanli A, Shenkin A, Imrie CW. The relationship between pancreatic enzyme release and activation and the acute-phase protein response in patients with acute pancreatitis. Pancreas. 1995 ; 10(4) : 347-53. https://doi.org/10.1097/00006676-199505000-00005