Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
권호 표지
DOI QR코드

DOI QR Code

NMR methods for structural analysis of RNA: a Review

  • Kim, Nak-Kyoon (Advanced Analysis Center, Korea Institute of Science and Technology) ;
  • Nam, Yun-Sik (Advanced Analysis Center, Korea Institute of Science and Technology) ;
  • Lee, Kang-Bong (Advanced Analysis Center, Korea Institute of Science and Technology)
  • Received : 2014.05.01
  • Accepted : 2014.06.10
  • Published : 2014.06.20
Download PDF
⟨ Previous Next ⟩

Abstract

In last three decades, RNA molecules have been revealed to play the central roles in many cellular processes. Structural understanding of RNA molecules is essential to interpret their functions, and many biophysical techniques have been adopted for this purpose. NMR spectroscopy is a powerful tool to study structures and dynamics of RNA molecules, and it has been further applied to study tertiary interactions between RNA molecules, RNA-protein, and RNA-small molecules. This short article accounts for the general methods for NMR sample preparations, and also partially covers the resonance assignments of structured RNA molecules.

Keywords

References

  1. J.F. Atkins, R.F. Gesteland, T.R. Cech, RNA worlds: From Life's origins to diversity in gene regulation (2010)
  2. M.V. Rodnina, Curr. Opin. Struct. Biol. 23, 595. (2013) https://doi.org/10.1016/j.sbi.2013.04.012
  3. J. Nandakumar, T.R.Cech, Nat. Rev. Mol. Cel.l Biol. 14, 69. (2013) https://doi.org/10.1038/nrm3505
  4. Y.-G. Choi, H.-E. Kim, J.-H Lee, J. Korean Magn. Reson. Soc. 17, 76. (2014) https://doi.org/10.6564/JKMRS.2013.17.2.076
  5. J.-H. Jeong, Y.-G. Park, S.-S. Choi, Y. Kim, J. Korean Magn. Reson. Soc. 17, 81. (2014) https://doi.org/10.6564/JKMRS.2013.17.2.081
  6. M. Zuker, Nucleic Acids Res. 31, 3406. (2003) https://doi.org/10.1093/nar/gkg595
  7. V. Sklenar, A. Bax, J. Magn. Reson. 74, 469. (1987)
  8. J. Wohnert, R. Ramachandran, M. Gorlach, L.R. Brown, J. Magn. Reson. 139, 430. (1999) https://doi.org/10.1006/jmre.1999.1797
  9. A.J. Dingley, L. Nisius, F. Cordier, S. Grzesiek, Nat. Protoc. 3, 242. (2008) https://doi.org/10.1038/nprot.2007.497
  10. J. Cromsigt, B. van Buuren, J. Schleucher, S. Wijmenga, Methods Enzymol. 338, 371. (2001)
  11. T.Dieckmann, J. Feigon, J. Biomol. NMR 9, 259. (1997) https://doi.org/10.1023/A:1018622708674
  12. R.D.Peterson, C.A.Theimer, H. Wu, J. Feigon, J. Biomol. NMR 28, 59. (2004) https://doi.org/10.1023/B:JNMR.0000012861.95939.05
  13. G.Varani, F. Aboulela, F.H.T. Allain, Prog. NMR Spectrosc. 29, 51. (1996) https://doi.org/10.1016/0079-6565(96)01028-X
  14. L.E.Kay, P.Keifer, T.Saarinen, J. Am. Chem. Soc. 114, 10663. (1992) https://doi.org/10.1021/ja00052a088

Cited by

  1. Structural Characterization of pre-miRNA 155 vol.20, pp.2, 2016, https://doi.org/10.6564/JKMRS.2016.20.2.046
  2. Proper NMR methods for studying RNA thermometers vol.19, pp.3, 2015, https://doi.org/10.6564/JKMRS.2015.19.3.143
  3. Hyperpolarization: Sensitivity Boost in Magnetic Resonance Spectroscopy and Imaging vol.19, pp.3, 2015, https://doi.org/10.6564/JKMRS.2015.19.3.124