Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Characteristics of Bacteria-Originated Keratinase for Feather Waste Treatment

가금폐기물 처리를 위한 세균유래 케라틴 분해효소의 특성

  • Go, Tae-Hun (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University) ;
  • Lee, Sang-Mee (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University) ;
  • Cho, Kwang-Sik (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University) ;
  • Lee, Ye-Ram (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University) ;
  • Park, Soo-Yun (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University) ;
  • Jang, Eun-Young (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University) ;
  • Jeong, Seong-Yun (Department of Medical Life Science, Catholic University of Daegu) ;
  • Son, Hong-Joo (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University)
  • 고태훈 (부산대학교 생명자원과학대학 및 생명산업융합연구원) ;
  • 이상미 (부산대학교 생명자원과학대학 및 생명산업융합연구원) ;
  • 조광식 (부산대학교 생명자원과학대학 및 생명산업융합연구원) ;
  • 이예람 (부산대학교 생명자원과학대학 및 생명산업융합연구원) ;
  • 박수연 (부산대학교 생명자원과학대학 및 생명산업융합연구원) ;
  • 장은영 (부산대학교 생명자원과학대학 및 생명산업융합연구원) ;
  • 정성윤 (대구가톨릭대 의생명과학과) ;
  • 손홍주 (부산대학교 생명자원과학대학 및 생명산업융합연구원)
  • Received : 2014.02.28
  • Accepted : 2014.05.21
  • Published : 2014.06.30
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Abstract

Keratin wastes are generated in excess of million tons per year worldwide and biodegradation of keratin by microorganisms possessing keratinase activity can be used as an alternative tool to prevent environmental pollution. For practical use of keratinase, its physicochemical properties should be investigated in detail. In this study, we investigated characteristics of keratinase produced by Xanthomonas sp. P5 which is isolated from rhizospheric soil of soybean. The level of keratinase produced by the strain P5 increased with time and reached its maximum (10.6 U/ml) at 3 days. The production of soluble protein had the same tendency as the production of keratinase. Optimal temperature and pH of keratinase were $40^{\circ}C-45^{\circ}C$ and pH 9, respectively. The enzyme showed broad temperature and pH stabilities. Thermostability profile showed that the enzyme retained 94.6%-100% of the original activity after 1 h treatment at $10^{\circ}C-40^{\circ}C$. After treatment for 1 h at pH 6-10, 89.2%-100% of the activity was remained. At pH 11, 71.6% of the original activity was retained after 1 h treatment. Although the strain P5 did not degrade human hair, it degraded duck feather and chicken feather. These results indicate that keratinase from Xanthomonas sp. P5 could be not only used to upgrade the nutritional value of feather hydrolysate but also useful in situ biodegradation of feather.

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References

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