Clinical and Experimental Reproductive Medicine

  • 제41권1호
  • /
  • Pages.1-8
  • /
  • 2014
  • /
  • 2233-8233(pISSN)
  • /
  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지
DOI QR코드

DOI QR Code

Effect of cell-penetrating peptide-conjugated estrogen-related receptor ${\beta}$ on the development of mouse embryos cultured in vitro

  • Yang, Ning Jie (Department of Biomedical Science, College of Life Science, CHA University) ;
  • Seol, Dong-Won (Department of Biomedical Science, College of Life Science, CHA University) ;
  • Jo, Junghyun (Department of Biomedical Science, College of Life Science, CHA University) ;
  • Jang, Hyun Mee (Department of Biomedical Science, College of Life Science, CHA University) ;
  • Yoon, Sook-Young (Department of Biomedical Science, College of Life Science, CHA University) ;
  • Lee, Dong Ryul (Department of Biomedical Science, College of Life Science, CHA University)
  • 투고 : 2013.12.26
  • 심사 : 2014.02.09
  • 발행 : 2014.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: Estrogen related receptor ${\beta}$ (Esrrb) is a member of the orphan nuclear receptors and may regulate the expression of pluripotencyrelated genes, such as Oct4 and Nanog. Therefore, in the present study, we have developed a method for delivering exogenous ESRRB recombinant protein into embryos by using cell-penetrating peptide (CPP) conjugation and have analyzed their effect on embryonic development. Methods: Mouse oocytes and embryos were obtained from superovulated mice. The expression of Oct4 mRNA and the cell number of inner cell mass (ICM) in the in vitro-derived and in vivo-derived blastocysts were first analyzed by real time-reverse transcription-polymerase chain reaction and differential staining. Then 8-cell embryos were cultured in KSOM media with or without $2{\mu}g/mL$ CPP-ESRRB protein for 24 to 48 hours, followed by checking their integration into embryos during in vitro culture by Western blot and immunocytochemistry. Results: Expression of Oct4 and the cell number of ICM were lower in the in vitro-derived blastocysts than in the in vivo-derived ones (p<0.05). In the blastocysts derived from the CPP-ESRRB-treated group, expression of Oct4 was greater than in the non-treated groups (p<0.05). Although no difference in embryonic development was observed between the treated and non-treated groups, the cell number of ICM was greater in the CPP-ESRRB-treated group. Conclusion: Treatment of CPP-ESRRB during cultivation could increase embryos' expression of Oct4 and the formation rate of the ICM in the blastocyst. Additionally, an exogenous delivery system of CPP-conjugated protein would be a useful tool for improving embryo culture systems.

키워드

참고문헌

  1. Goldberg JM, Khalifa EA, Friedman CI, Kim MH. Improvement of in vitro fertilization and early embryo development in mice by coculture with human fallopian tube epithelium. Am J Obstet Gynecol 1991;165:1802-5. https://doi.org/10.1016/0002-9378(91)90035-P
  2. Freeman MR, Bastias MC, Hill GA, Osteen KG. Coculture of mouse embryos with cells isolated from the human ovarian follicle, oviduct, and uterine endometrium. Fertil Steril 1993;59:138-42. https://doi.org/10.1016/S0015-0282(16)55629-5
  3. Saito H, Hirayama T, Koike K, Saito T, Nohara M, Hiroi M. Cumulus mass maintains embryo quality. Fertil Steril 1994;62:555-8. https://doi.org/10.1016/S0015-0282(16)56945-3
  4. Paria BC, Dey SK. Preimplantation embryo development in vitro: cooperative interactions among embryos and role of growth factors. Proc Natl Acad Sci USA 1990;87:4756-60. https://doi.org/10.1073/pnas.87.12.4756
  5. Morita Y, Tsutsumi O, Taketani Y. In vitro treatment of embryos with epidermal growth factor improves viability and increases the implantation rate of blastocysts transferred to recipient mice. Am J Obstet Gynecol 1994;171:406-9. https://doi.org/10.1016/S0002-9378(94)70042-7
  6. Harvey MB, Kaye PL. Insulin-like growth factor-1 stimulates growth of mouse preimplantation embryos in vitro. Mol Reprod Dev 1992;31:195-9. https://doi.org/10.1002/mrd.1080310306
  7. Rappolee DA, Sturm KS, Behrendtsen O, Schultz GA, Pedersen RA, Werb Z. Insulin-like growth factor II acts through an endogenous growth pathway regulated by imprinting in early mouse embryos. Genes Dev 1992;6:939-52. https://doi.org/10.1101/gad.6.6.939
  8. Lavranos TC, Rathjen PD, Seamark RF. Trophic effects of myeloid leukaemia inhibitory factor (LIF) on mouse embryos. J Reprod Fertil 1995;105:331-8. https://doi.org/10.1530/jrf.0.1050331
  9. Zhang X, Zhang J, Wang T, Esteban MA, Pei D. Esrrb activates Oct4 transcription and sustains self-renewal and pluripotency in embryonic stem cells. J Biol Chem 2008;283:35825-33. https://doi.org/10.1074/jbc.M803481200
  10. Ovitt CE, Scholer HR. The molecular biology of Oct-4 in the early mouse embryo. Mol Hum Reprod 1998;4:1021-31. https://doi.org/10.1093/molehr/4.11.1021
  11. Ivanova N, Dobrin R, Lu R, Kotenko I, Levorse J, DeCoste C, et al. Dissecting self-renewal in stem cells with RNA interference. Nature 2006;442:533-8. https://doi.org/10.1038/nature04915
  12. Luo J, Sladek R, Bader JA, Matthyssen A, Rossant J, Giguere V. Placental abnormalities in mouse embryos lacking the orphan nuclear receptor ERR-beta. Nature 1997;388:778-82. https://doi.org/10.1038/42022
  13. Jo J, Song H, Park SG, Lee SH, Ko JJ, Park JH, et al. Regulation of differentiation potential of human mesenchymal stem cells by intracytoplasmic delivery of coactivator-associated arginine methyltransferase 1 protein using cell-penetrating peptide. Stem Cells 2012;30:1703-13. https://doi.org/10.1002/stem.1146
  14. Choi S, Jo J, Seol DW, Cha SK, Lee JE, Lee DR. Regulation of pluripotency- related genes and differentiation in mouse embryonic stem cells by direct delivery of cell-penetrating peptide-conjugated CARM1 recombinant protein. Dev Reprod 2013;17:9-16. https://doi.org/10.12717/DR.2013.17.1.009
  15. Jo J, Lee Y, Oh MH, Ko JJ, Cheon YP, Lee DR. Up-regulation of pluripotency- related Genes in human amniotic fluid-derived stem cells by ESRRB conjugated with Cell-Penetrating Peptide. Dev Reprod 2010;14:243-51.
  16. Farin CE, Farmer WT, Farin PW. Pregnancy recognition and abnormal offspring syndrome in cattle. Reprod Fertil Dev 2010;22:75-87.
  17. Kiessling AA, Davis HW, Williams CS, Sauter RW, Harrison LW. Development and DNA polymerase activities in cultured preimplantation mouse embryos: comparison with embryos developed in vivo. J Exp Zool 1991;258:34-47. https://doi.org/10.1002/jez.1402580105
  18. Zhou W, Liu Z, Wu J, Liu JH, Hyder SM, Antoniou E, et al. Identification and characterization of two novel splicing isoforms of human estrogen-related receptor beta. J Clin Endocrinol Metab 2006;91:569-79. https://doi.org/10.1210/jc.2004-1957
  19. Giguere V, Yang N, Segui P, Evans RM. Identification of a new class of steroid hormone receptors. Nature 1988;331:91-4. https://doi.org/10.1038/331091a0
  20. Bombail V, MacPherson S, Critchley HO, Saunders PT. Estrogen receptor related beta is expressed in human endometrium throughout the normal menstrual cycle. Hum Reprod 2008;23:2782-90. https://doi.org/10.1093/humrep/den298
  21. van den Berg DL, Zhang W, Yates A, Engelen E, Takacs K, Bezstarosti K, et al. Estrogen-related receptor beta interacts with Oct4 to positively regulate Nanog gene expression. Mol Cell Biol 2008;28:5986-95. https://doi.org/10.1128/MCB.00301-08
  22. Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, et al. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 1998;95:379-91. https://doi.org/10.1016/S0092-8674(00)81769-9
  23. Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 2000;24:372-6. https://doi.org/10.1038/74199
  24. Patel LN, Zaro JL, Shen WC. Cell penetrating peptides: intracellular pathways and pharmaceutical perspectives. Pharm Res 2007;24:1977-92. https://doi.org/10.1007/s11095-007-9303-7
  25. Kueltzo LA, Middaugh CR. Structural characterization of bovine granulocyte colony stimulating factor: effect of temperature and pH. J Pharm Sci 2003;92:1793-804. https://doi.org/10.1002/jps.10440
  26. Frankel AD, Pabo CO. Cellular uptake of the tat protein from human immunodeficiency virus. Cell 1988;55:1189-93. https://doi.org/10.1016/0092-8674(88)90263-2

피인용 문헌

  1. Extracellular vesicle-mediated delivery of molecular compounds into gametes and embryos: learning from nature vol.21, pp.5, 2015, https://doi.org/10.1093/humupd/dmv027
  2. Supplementation With Cell-Penetrating Peptide-Conjugated Estrogen-Related Receptor β Improves the Formation of the Inner Cell Mass and the Development of Vitrified/Warmed Mouse Embryos vol.23, pp.11, 2014, https://doi.org/10.1177/1933719116643594
  3. The effect of cell penetrating peptide-conjugated coactivator-associated arginine methyltransferase 1 (CPP-CARM1) on the cloned mouse embryonic development vol.8, pp.None, 2014, https://doi.org/10.1038/s41598-018-35077-0