Asian Pacific Journal of Cancer Prevention

  • 제15권3호
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  • Pages.1397-1401
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  • 2014
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Expression of bcl-2 and p53 in Induction of Esophageal Cancer Cell Apoptosis by ECRG2 in Combination with Cisplatin

  • Song, Hai-Yan (College of Basic Medicine, Xinxiang Medical University, Key Laboratory for Medical Tissue Regeneration of Henan Province) ;
  • Deng, Xiao-Hui (College of Basic Medicine, Xinxiang Medical University, Key Laboratory for Medical Tissue Regeneration of Henan Province) ;
  • Yuan, Guo-Yan (College of Basic Medicine, Xinxiang Medical University, Key Laboratory for Medical Tissue Regeneration of Henan Province) ;
  • Hou, Xin-Fang (The Affiliated Cancer Hospital of Zhengzhou University) ;
  • Zhu, Zhen-Dong (College of Basic Medicine, Xinxiang Medical University, Key Laboratory for Medical Tissue Regeneration of Henan Province) ;
  • Zhou, Li (College of Basic Medicine, Xinxiang Medical University, Key Laboratory for Medical Tissue Regeneration of Henan Province) ;
  • Ren, Ming-Xin (College of Basic Medicine, Xinxiang Medical University, Key Laboratory for Medical Tissue Regeneration of Henan Province)
  • 발행 : 2014.02.01
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초록

Aim: To investigate the mechanisms of induction of apoptosis of esophageal cancer cells by esophageal cancer-related gene 2 (ECRG2) in combination with cisplatin (DDP). Methods: Hoechest staining was performed to analyze the effects of single ECRG2 and ECRG2 in combination with DDP on apoptosis of EC9706 cells. The expression levels of p53 and bcl-2 mRNA and protein were determined by RT-PCR and Western blotting, respectively. Results: The number of apoptotic cells after the treatment with ECRG2 in combination with DDP for 24 hours was more than that after the treatment with single ECRG2. RT-PCR and Western blotting showed that the expression levels of bcl-2 mRNA and protein were both down-regulated, while p53 mRNA and protein were both up-regulated in the cells treated with ECRG2 in combination with DDP compared with those given ECRG2 alone. Conclusion: ECRG2 in combination with DDP can enhance the apoptosis of EC9706 cells, possibly by down-regulating bcl-2 expression and up-regulating p53.

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참고문헌

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